Job ID = 11634624 sra ファイルのダウンロード中... Completed: 488868K bytes transferred in 7 seconds (527715K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 12143550 spots for /home/okishinya/chipatlas/results/sacCer3/ERX671085/ERR727166.sra Written 12143550 spots for /home/okishinya/chipatlas/results/sacCer3/ERX671085/ERR727166.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:29 12143550 reads; of these: 12143550 (100.00%) were unpaired; of these: 832593 (6.86%) aligned 0 times 9831192 (80.96%) aligned exactly 1 time 1479765 (12.19%) aligned >1 times 93.14% overall alignment rate Time searching: 00:01:29 Overall time: 00:01:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3484943 / 11310957 = 0.3081 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 10:20:44: # Command line: callpeak -t ERX671085.bam -f BAM -g 12100000 -n ERX671085.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX671085.20 # format = BAM # ChIP-seq file = ['ERX671085.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:20:44: # Command line: callpeak -t ERX671085.bam -f BAM -g 12100000 -n ERX671085.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX671085.05 # format = BAM # ChIP-seq file = ['ERX671085.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:20:44: # Command line: callpeak -t ERX671085.bam -f BAM -g 12100000 -n ERX671085.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX671085.10 # format = BAM # ChIP-seq file = ['ERX671085.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 10:20:44: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:20:44: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:20:44: #1 read tag files... INFO @ Fri, 15 Feb 2019 10:20:44: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:20:44: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:20:44: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 10:20:49: 1000000 INFO @ Fri, 15 Feb 2019 10:20:50: 1000000 INFO @ Fri, 15 Feb 2019 10:20:50: 1000000 INFO @ Fri, 15 Feb 2019 10:20:55: 2000000 INFO @ Fri, 15 Feb 2019 10:20:55: 2000000 INFO @ Fri, 15 Feb 2019 10:20:56: 2000000 INFO @ Fri, 15 Feb 2019 10:21:00: 3000000 INFO @ Fri, 15 Feb 2019 10:21:01: 3000000 INFO @ Fri, 15 Feb 2019 10:21:01: 3000000 INFO @ Fri, 15 Feb 2019 10:21:06: 4000000 INFO @ Fri, 15 Feb 2019 10:21:07: 4000000 INFO @ Fri, 15 Feb 2019 10:21:07: 4000000 INFO @ Fri, 15 Feb 2019 10:21:11: 5000000 INFO @ Fri, 15 Feb 2019 10:21:13: 5000000 INFO @ Fri, 15 Feb 2019 10:21:13: 5000000 INFO @ Fri, 15 Feb 2019 10:21:17: 6000000 INFO @ Fri, 15 Feb 2019 10:21:19: 6000000 INFO @ Fri, 15 Feb 2019 10:21:19: 6000000 INFO @ Fri, 15 Feb 2019 10:21:22: 7000000 INFO @ Fri, 15 Feb 2019 10:21:24: 7000000 INFO @ Fri, 15 Feb 2019 10:21:25: 7000000 INFO @ Fri, 15 Feb 2019 10:21:27: #1 tag size is determined as 32 bps INFO @ Fri, 15 Feb 2019 10:21:27: #1 tag size = 32 INFO @ Fri, 15 Feb 2019 10:21:27: #1 total tags in treatment: 7826014 INFO @ Fri, 15 Feb 2019 10:21:27: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:21:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:21:27: #1 tags after filtering in treatment: 7826014 INFO @ Fri, 15 Feb 2019 10:21:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:21:27: #1 finished! INFO @ Fri, 15 Feb 2019 10:21:27: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:21:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:21:27: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:21:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:21:27: Process for pairing-model is terminated! cat: ERX671085.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX671085.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX671085.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX671085.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:21:29: #1 tag size is determined as 32 bps INFO @ Fri, 15 Feb 2019 10:21:29: #1 tag size = 32 INFO @ Fri, 15 Feb 2019 10:21:29: #1 total tags in treatment: 7826014 INFO @ Fri, 15 Feb 2019 10:21:29: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:21:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:21:29: #1 tags after filtering in treatment: 7826014 INFO @ Fri, 15 Feb 2019 10:21:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:21:29: #1 finished! INFO @ Fri, 15 Feb 2019 10:21:29: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:21:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:21:30: #1 tag size is determined as 32 bps INFO @ Fri, 15 Feb 2019 10:21:30: #1 tag size = 32 INFO @ Fri, 15 Feb 2019 10:21:30: #1 total tags in treatment: 7826014 INFO @ Fri, 15 Feb 2019 10:21:30: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 10:21:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 10:21:30: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:21:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:21:30: Process for pairing-model is terminated! cat: ERX671085.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX671085.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX671085.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX671085.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 10:21:30: #1 tags after filtering in treatment: 7826014 INFO @ Fri, 15 Feb 2019 10:21:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 10:21:30: #1 finished! INFO @ Fri, 15 Feb 2019 10:21:30: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 10:21:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 10:21:30: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 10:21:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 10:21:30: Process for pairing-model is terminated! cat: ERX671085.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX671085.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX671085.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX671085.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。