Job ID = 5790829 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 7,448,556 reads read : 10,439,796 reads written : 10,439,796 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 7509 / 9087 = 0.8263 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:00:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:00:29: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:00:29: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:00:32: 1000000 INFO @ Wed, 22 Apr 2020 08:00:36: 2000000 INFO @ Wed, 22 Apr 2020 08:00:40: #1 tag size is determined as 32 bps INFO @ Wed, 22 Apr 2020 08:00:40: #1 tag size = 32 INFO @ Wed, 22 Apr 2020 08:00:40: #1 total tags in treatment: 1494 INFO @ Wed, 22 Apr 2020 08:00:40: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:00:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:00:40: #1 tags after filtering in treatment: 1451 INFO @ Wed, 22 Apr 2020 08:00:40: #1 Redundant rate of treatment: 0.03 INFO @ Wed, 22 Apr 2020 08:00:40: #1 finished! INFO @ Wed, 22 Apr 2020 08:00:40: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:00:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:00:40: #2 number of paired peaks: 11 WARNING @ Wed, 22 Apr 2020 08:00:40: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:00:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:00:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:00:58: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:00:58: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:01:02: 1000000 INFO @ Wed, 22 Apr 2020 08:01:06: 2000000 INFO @ Wed, 22 Apr 2020 08:01:09: #1 tag size is determined as 32 bps INFO @ Wed, 22 Apr 2020 08:01:09: #1 tag size = 32 INFO @ Wed, 22 Apr 2020 08:01:09: #1 total tags in treatment: 1494 INFO @ Wed, 22 Apr 2020 08:01:09: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:01:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:01:09: #1 tags after filtering in treatment: 1451 INFO @ Wed, 22 Apr 2020 08:01:09: #1 Redundant rate of treatment: 0.03 INFO @ Wed, 22 Apr 2020 08:01:09: #1 finished! INFO @ Wed, 22 Apr 2020 08:01:09: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:01:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:01:09: #2 number of paired peaks: 11 WARNING @ Wed, 22 Apr 2020 08:01:09: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:01:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 08:01:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 08:01:28: #1 read tag files... INFO @ Wed, 22 Apr 2020 08:01:28: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 08:01:32: 1000000 INFO @ Wed, 22 Apr 2020 08:01:36: 2000000 INFO @ Wed, 22 Apr 2020 08:01:40: #1 tag size is determined as 32 bps INFO @ Wed, 22 Apr 2020 08:01:40: #1 tag size = 32 INFO @ Wed, 22 Apr 2020 08:01:40: #1 total tags in treatment: 1494 INFO @ Wed, 22 Apr 2020 08:01:40: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 08:01:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 08:01:40: #1 tags after filtering in treatment: 1451 INFO @ Wed, 22 Apr 2020 08:01:40: #1 Redundant rate of treatment: 0.03 INFO @ Wed, 22 Apr 2020 08:01:40: #1 finished! INFO @ Wed, 22 Apr 2020 08:01:40: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 08:01:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 08:01:40: #2 number of paired peaks: 11 WARNING @ Wed, 22 Apr 2020 08:01:40: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 08:01:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX671079/ERX671079.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。