Job ID = 2009016 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,463,302 reads read : 4,463,302 reads written : 4,463,302 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR637548.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:37 4463302 reads; of these: 4463302 (100.00%) were unpaired; of these: 593202 (13.29%) aligned 0 times 3324937 (74.50%) aligned exactly 1 time 545163 (12.21%) aligned >1 times 86.71% overall alignment rate Time searching: 00:00:37 Overall time: 00:00:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 964968 / 3870100 = 0.2493 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:10:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:10:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:10:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:10:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:10:23: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:10:23: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:10:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:10:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:10:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:10:29: 1000000 INFO @ Fri, 05 Jul 2019 19:10:31: 1000000 INFO @ Fri, 05 Jul 2019 19:10:31: 1000000 INFO @ Fri, 05 Jul 2019 19:10:37: 2000000 INFO @ Fri, 05 Jul 2019 19:10:37: 2000000 INFO @ Fri, 05 Jul 2019 19:10:39: 2000000 INFO @ Fri, 05 Jul 2019 19:10:43: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:10:43: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:10:43: #1 total tags in treatment: 2905132 INFO @ Fri, 05 Jul 2019 19:10:43: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:10:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:10:43: #1 tags after filtering in treatment: 2905132 INFO @ Fri, 05 Jul 2019 19:10:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:10:43: #1 finished! INFO @ Fri, 05 Jul 2019 19:10:43: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:10:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:10:43: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 19:10:43: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:10:43: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:10:44: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:10:44: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:10:44: #1 total tags in treatment: 2905132 INFO @ Fri, 05 Jul 2019 19:10:44: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:10:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:10:44: #1 tags after filtering in treatment: 2905132 INFO @ Fri, 05 Jul 2019 19:10:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:10:44: #1 finished! INFO @ Fri, 05 Jul 2019 19:10:44: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:10:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:10:44: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 19:10:44: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:10:44: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:10:45: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:10:45: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:10:45: #1 total tags in treatment: 2905132 INFO @ Fri, 05 Jul 2019 19:10:45: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:10:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:10:45: #1 tags after filtering in treatment: 2905132 INFO @ Fri, 05 Jul 2019 19:10:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:10:45: #1 finished! INFO @ Fri, 05 Jul 2019 19:10:45: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:10:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:10:46: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 19:10:46: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:10:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.05_peaks.narrowPeakcut: : No such file or directory/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.10_peaks.narrowPeak : No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.10_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: CompletedMACS2peakCalling cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594032/ERX594032.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。