Job ID = 2008994 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,100,761 reads read : 5,100,761 reads written : 5,100,761 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR637579.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:21 5100761 reads; of these: 5100761 (100.00%) were unpaired; of these: 3822692 (74.94%) aligned 0 times 742504 (14.56%) aligned exactly 1 time 535565 (10.50%) aligned >1 times 25.06% overall alignment rate Time searching: 00:00:21 Overall time: 00:00:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 615035 / 1278069 = 0.4812 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 05 Jul 2019 19:01:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:01:44: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:01:44: #1 read treatment tags... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:01:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:01:45: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:01:45: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:01:48: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:01:48: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:01:48: #1 total tags in treatment: 663034 INFO @ Fri, 05 Jul 2019 19:01:48: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:01:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:01:48: #1 tags after filtering in treatment: 663034 INFO @ Fri, 05 Jul 2019 19:01:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:01:48: #1 finished! INFO @ Fri, 05 Jul 2019 19:01:48: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:01:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:01:48: #2 number of paired peaks: 305 WARNING @ Fri, 05 Jul 2019 19:01:48: Fewer paired peaks (305) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 305 pairs to build model! INFO @ Fri, 05 Jul 2019 19:01:48: start model_add_line... INFO @ Fri, 05 Jul 2019 19:01:48: start X-correlation... INFO @ Fri, 05 Jul 2019 19:01:48: end of X-cor INFO @ Fri, 05 Jul 2019 19:01:48: #2 finished! INFO @ Fri, 05 Jul 2019 19:01:48: #2 predicted fragment length is 153 bps INFO @ Fri, 05 Jul 2019 19:01:48: #2 alternative fragment length(s) may be 153 bps INFO @ Fri, 05 Jul 2019 19:01:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.05_model.r INFO @ Fri, 05 Jul 2019 19:02:20: #3 Call peaks... INFO @ Fri, 05 Jul 2019 19:02:20: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 19:02:22: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 19:02:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:02:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:02:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:02:22: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.05_peaks.xls INFO @ Fri, 05 Jul 2019 19:02:23: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:02:23: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:02:23: #1 total tags in treatment: 663034 INFO @ Fri, 05 Jul 2019 19:02:23: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:02:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:02:23: #1 tags after filtering in treatment: 663034 INFO @ Fri, 05 Jul 2019 19:02:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:02:23: #1 finished! INFO @ Fri, 05 Jul 2019 19:02:23: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:02:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:02:23: #2 number of paired peaks: 305 WARNING @ Fri, 05 Jul 2019 19:02:23: Fewer paired peaks (305) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 305 pairs to build model! INFO @ Fri, 05 Jul 2019 19:02:23: start model_add_line... INFO @ Fri, 05 Jul 2019 19:02:24: start X-correlation... INFO @ Fri, 05 Jul 2019 19:02:24: end of X-cor INFO @ Fri, 05 Jul 2019 19:02:24: #2 finished! INFO @ Fri, 05 Jul 2019 19:02:24: #2 predicted fragment length is 153 bps INFO @ Fri, 05 Jul 2019 19:02:24: #2 alternative fragment length(s) may be 153 bps INFO @ Fri, 05 Jul 2019 19:02:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.10_model.r INFO @ Fri, 05 Jul 2019 19:02:26: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 19:02:26: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 19:02:26: #1 total tags in treatment: 663034 INFO @ Fri, 05 Jul 2019 19:02:26: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:02:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:02:26: #1 tags after filtering in treatment: 663034 INFO @ Fri, 05 Jul 2019 19:02:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:02:26: #1 finished! INFO @ Fri, 05 Jul 2019 19:02:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:02:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:02:26: #2 number of paired peaks: 305 WARNING @ Fri, 05 Jul 2019 19:02:26: Fewer paired peaks (305) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 305 pairs to build model! INFO @ Fri, 05 Jul 2019 19:02:26: start model_add_line... INFO @ Fri, 05 Jul 2019 19:02:26: start X-correlation... INFO @ Fri, 05 Jul 2019 19:02:26: end of X-cor INFO @ Fri, 05 Jul 2019 19:02:26: #2 finished! INFO @ Fri, 05 Jul 2019 19:02:26: #2 predicted fragment length is 153 bps INFO @ Fri, 05 Jul 2019 19:02:26: #2 alternative fragment length(s) may be 153 bps INFO @ Fri, 05 Jul 2019 19:02:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.20_model.r INFO @ Fri, 05 Jul 2019 19:02:27: #3 Call peaks... INFO @ Fri, 05 Jul 2019 19:02:27: #3 Call peaks... INFO @ Fri, 05 Jul 2019 19:02:27: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 19:02:27: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 19:02:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 19:02:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.05_summits.bed INFO @ Fri, 05 Jul 2019 19:02:27: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (1233 records, 4 fields): 2 millis INFO @ Fri, 05 Jul 2019 19:02:28: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 19:02:28: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 19:02:29: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.10_peaks.xls INFO @ Fri, 05 Jul 2019 19:02:29: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.20_peaks.xls INFO @ Fri, 05 Jul 2019 19:02:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 19:02:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 19:02:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.20_summits.bed INFO @ Fri, 05 Jul 2019 19:02:29: Done! INFO @ Fri, 05 Jul 2019 19:02:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX594014/ERX594014.10_summits.bed INFO @ Fri, 05 Jul 2019 19:02:29: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (312 records, 4 fields): 1 millis pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (611 records, 4 fields): 2 millis BedGraph に変換しました。 BigWig に変換中... CompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling BigWig に変換しました。