Job ID = 2008341 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 21,480,277 reads read : 42,960,554 reads written : 42,960,554 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR629025.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:29 21480277 reads; of these: 21480277 (100.00%) were paired; of these: 2096645 (9.76%) aligned concordantly 0 times 18158248 (84.53%) aligned concordantly exactly 1 time 1225384 (5.70%) aligned concordantly >1 times ---- 2096645 pairs aligned concordantly 0 times; of these: 621509 (29.64%) aligned discordantly 1 time ---- 1475136 pairs aligned 0 times concordantly or discordantly; of these: 2950272 mates make up the pairs; of these: 2766605 (93.77%) aligned 0 times 95987 (3.25%) aligned exactly 1 time 87680 (2.97%) aligned >1 times 93.56% overall alignment rate Time searching: 00:32:29 Overall time: 00:32:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5022184 / 19914605 = 0.2522 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:50:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:23: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:23: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:23: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:23: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:50:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:50:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:50:32: 1000000 INFO @ Fri, 05 Jul 2019 19:50:34: 1000000 INFO @ Fri, 05 Jul 2019 19:50:34: 1000000 INFO @ Fri, 05 Jul 2019 19:50:41: 2000000 INFO @ Fri, 05 Jul 2019 19:50:43: 2000000 INFO @ Fri, 05 Jul 2019 19:50:44: 2000000 INFO @ Fri, 05 Jul 2019 19:50:50: 3000000 INFO @ Fri, 05 Jul 2019 19:50:52: 3000000 INFO @ Fri, 05 Jul 2019 19:50:55: 3000000 INFO @ Fri, 05 Jul 2019 19:50:59: 4000000 INFO @ Fri, 05 Jul 2019 19:51:02: 4000000 INFO @ Fri, 05 Jul 2019 19:51:05: 4000000 INFO @ Fri, 05 Jul 2019 19:51:08: 5000000 INFO @ Fri, 05 Jul 2019 19:51:11: 5000000 INFO @ Fri, 05 Jul 2019 19:51:15: 5000000 INFO @ Fri, 05 Jul 2019 19:51:16: 6000000 INFO @ Fri, 05 Jul 2019 19:51:20: 6000000 INFO @ Fri, 05 Jul 2019 19:51:25: 7000000 INFO @ Fri, 05 Jul 2019 19:51:26: 6000000 INFO @ Fri, 05 Jul 2019 19:51:30: 7000000 INFO @ Fri, 05 Jul 2019 19:51:35: 8000000 INFO @ Fri, 05 Jul 2019 19:51:36: 7000000 INFO @ Fri, 05 Jul 2019 19:51:39: 8000000 INFO @ Fri, 05 Jul 2019 19:51:43: 9000000 INFO @ Fri, 05 Jul 2019 19:51:47: 8000000 INFO @ Fri, 05 Jul 2019 19:51:48: 9000000 INFO @ Fri, 05 Jul 2019 19:51:52: 10000000 INFO @ Fri, 05 Jul 2019 19:51:57: 9000000 INFO @ Fri, 05 Jul 2019 19:51:58: 10000000 INFO @ Fri, 05 Jul 2019 19:52:01: 11000000 INFO @ Fri, 05 Jul 2019 19:52:06: 10000000 INFO @ Fri, 05 Jul 2019 19:52:07: 11000000 INFO @ Fri, 05 Jul 2019 19:52:10: 12000000 INFO @ Fri, 05 Jul 2019 19:52:16: 12000000 INFO @ Fri, 05 Jul 2019 19:52:17: 11000000 INFO @ Fri, 05 Jul 2019 19:52:19: 13000000 INFO @ Fri, 05 Jul 2019 19:52:25: 13000000 INFO @ Fri, 05 Jul 2019 19:52:27: 12000000 INFO @ Fri, 05 Jul 2019 19:52:27: 14000000 INFO @ Fri, 05 Jul 2019 19:52:34: 14000000 INFO @ Fri, 05 Jul 2019 19:52:36: 15000000 INFO @ Fri, 05 Jul 2019 19:52:37: 13000000 INFO @ Fri, 05 Jul 2019 19:52:43: 15000000 INFO @ Fri, 05 Jul 2019 19:52:44: 16000000 INFO @ Fri, 05 Jul 2019 19:52:47: 14000000 INFO @ Fri, 05 Jul 2019 19:52:52: 16000000 INFO @ Fri, 05 Jul 2019 19:52:53: 17000000 INFO @ Fri, 05 Jul 2019 19:52:56: 15000000 INFO @ Fri, 05 Jul 2019 19:53:01: 17000000 INFO @ Fri, 05 Jul 2019 19:53:02: 18000000 INFO @ Fri, 05 Jul 2019 19:53:07: 16000000 INFO @ Fri, 05 Jul 2019 19:53:10: 18000000 INFO @ Fri, 05 Jul 2019 19:53:10: 19000000 INFO @ Fri, 05 Jul 2019 19:53:17: 17000000 INFO @ Fri, 05 Jul 2019 19:53:19: 19000000 INFO @ Fri, 05 Jul 2019 19:53:19: 20000000 INFO @ Fri, 05 Jul 2019 19:53:27: 18000000 INFO @ Fri, 05 Jul 2019 19:53:28: 21000000 INFO @ Fri, 05 Jul 2019 19:53:28: 20000000 INFO @ Fri, 05 Jul 2019 19:53:37: 22000000 INFO @ Fri, 05 Jul 2019 19:53:37: 19000000 INFO @ Fri, 05 Jul 2019 19:53:37: 21000000 INFO @ Fri, 05 Jul 2019 19:53:46: 23000000 INFO @ Fri, 05 Jul 2019 19:53:46: 22000000 INFO @ Fri, 05 Jul 2019 19:53:47: 20000000 INFO @ Fri, 05 Jul 2019 19:53:54: 24000000 INFO @ Fri, 05 Jul 2019 19:53:56: 23000000 INFO @ Fri, 05 Jul 2019 19:53:58: 21000000 INFO @ Fri, 05 Jul 2019 19:54:02: 25000000 INFO @ Fri, 05 Jul 2019 19:54:05: 24000000 INFO @ Fri, 05 Jul 2019 19:54:08: 22000000 INFO @ Fri, 05 Jul 2019 19:54:11: 26000000 INFO @ Fri, 05 Jul 2019 19:54:13: 25000000 INFO @ Fri, 05 Jul 2019 19:54:19: 23000000 INFO @ Fri, 05 Jul 2019 19:54:20: 27000000 INFO @ Fri, 05 Jul 2019 19:54:22: 26000000 INFO @ Fri, 05 Jul 2019 19:54:28: 28000000 INFO @ Fri, 05 Jul 2019 19:54:29: 24000000 INFO @ Fri, 05 Jul 2019 19:54:31: 27000000 INFO @ Fri, 05 Jul 2019 19:54:37: 29000000 INFO @ Fri, 05 Jul 2019 19:54:39: 25000000 INFO @ Fri, 05 Jul 2019 19:54:40: 28000000 INFO @ Fri, 05 Jul 2019 19:54:45: 30000000 INFO @ Fri, 05 Jul 2019 19:54:47: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 19:54:47: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 19:54:47: #1 total tags in treatment: 14421101 INFO @ Fri, 05 Jul 2019 19:54:47: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:54:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:54:47: #1 tags after filtering in treatment: 5841450 INFO @ Fri, 05 Jul 2019 19:54:47: #1 Redundant rate of treatment: 0.59 INFO @ Fri, 05 Jul 2019 19:54:47: #1 finished! INFO @ Fri, 05 Jul 2019 19:54:47: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:54:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:54:48: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:54:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:54:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:54:48: 26000000 INFO @ Fri, 05 Jul 2019 19:54:49: 29000000 INFO @ Fri, 05 Jul 2019 19:54:58: 30000000 INFO @ Fri, 05 Jul 2019 19:54:59: 27000000 INFO @ Fri, 05 Jul 2019 19:54:59: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 19:54:59: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 19:54:59: #1 total tags in treatment: 14421101 INFO @ Fri, 05 Jul 2019 19:54:59: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:54:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:55:00: #1 tags after filtering in treatment: 5841450 INFO @ Fri, 05 Jul 2019 19:55:00: #1 Redundant rate of treatment: 0.59 INFO @ Fri, 05 Jul 2019 19:55:00: #1 finished! INFO @ Fri, 05 Jul 2019 19:55:00: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:55:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:55:00: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:55:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:55:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 587 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:55:09: 28000000 INFO @ Fri, 05 Jul 2019 19:55:18: 29000000 INFO @ Fri, 05 Jul 2019 19:55:28: 30000000 INFO @ Fri, 05 Jul 2019 19:55:30: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 19:55:30: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 19:55:30: #1 total tags in treatment: 14421101 INFO @ Fri, 05 Jul 2019 19:55:30: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:55:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:55:30: #1 tags after filtering in treatment: 5841450 INFO @ Fri, 05 Jul 2019 19:55:30: #1 Redundant rate of treatment: 0.59 INFO @ Fri, 05 Jul 2019 19:55:30: #1 finished! INFO @ Fri, 05 Jul 2019 19:55:30: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:55:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:55:31: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:55:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:55:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585847/ERX585847.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。