Job ID = 2008327 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 19,859,882 reads read : 39,719,764 reads written : 39,719,764 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR629035.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:35 19859882 reads; of these: 19859882 (100.00%) were paired; of these: 773241 (3.89%) aligned concordantly 0 times 17889397 (90.08%) aligned concordantly exactly 1 time 1197244 (6.03%) aligned concordantly >1 times ---- 773241 pairs aligned concordantly 0 times; of these: 81192 (10.50%) aligned discordantly 1 time ---- 692049 pairs aligned 0 times concordantly or discordantly; of these: 1384098 mates make up the pairs; of these: 1290442 (93.23%) aligned 0 times 70346 (5.08%) aligned exactly 1 time 23310 (1.68%) aligned >1 times 96.75% overall alignment rate Time searching: 00:19:35 Overall time: 00:19:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 17654953 / 19156550 = 0.9216 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 05 Jul 2019 19:06:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:06:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:06:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:06:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:06:31: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:06:31: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:06:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:06:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:06:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:06:38: 1000000 INFO @ Fri, 05 Jul 2019 19:06:38: 1000000 INFO @ Fri, 05 Jul 2019 19:06:39: 1000000 INFO @ Fri, 05 Jul 2019 19:06:45: 2000000 INFO @ Fri, 05 Jul 2019 19:06:46: 2000000 INFO @ Fri, 05 Jul 2019 19:06:47: 2000000 INFO @ Fri, 05 Jul 2019 19:06:51: 3000000 INFO @ Fri, 05 Jul 2019 19:06:52: #1 tag size is determined as 44 bps INFO @ Fri, 05 Jul 2019 19:06:52: #1 tag size = 44 INFO @ Fri, 05 Jul 2019 19:06:52: #1 total tags in treatment: 1456572 INFO @ Fri, 05 Jul 2019 19:06:52: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:06:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:06:52: #1 tags after filtering in treatment: 1074474 INFO @ Fri, 05 Jul 2019 19:06:52: #1 Redundant rate of treatment: 0.26 INFO @ Fri, 05 Jul 2019 19:06:52: #1 finished! INFO @ Fri, 05 Jul 2019 19:06:52: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:06:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:06:52: #2 number of paired peaks: 58 WARNING @ Fri, 05 Jul 2019 19:06:52: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:06:52: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:06:54: 3000000 INFO @ Fri, 05 Jul 2019 19:06:55: #1 tag size is determined as 44 bps INFO @ Fri, 05 Jul 2019 19:06:55: #1 tag size = 44 INFO @ Fri, 05 Jul 2019 19:06:55: #1 total tags in treatment: 1456572 INFO @ Fri, 05 Jul 2019 19:06:55: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:06:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:06:55: #1 tags after filtering in treatment: 1074474 INFO @ Fri, 05 Jul 2019 19:06:55: #1 Redundant rate of treatment: 0.26 INFO @ Fri, 05 Jul 2019 19:06:55: #1 finished! INFO @ Fri, 05 Jul 2019 19:06:55: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:06:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:06:55: #2 number of paired peaks: 58 WARNING @ Fri, 05 Jul 2019 19:06:55: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:06:55: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:06:55: 3000000 INFO @ Fri, 05 Jul 2019 19:06:56: #1 tag size is determined as 44 bps INFO @ Fri, 05 Jul 2019 19:06:56: #1 tag size = 44 INFO @ Fri, 05 Jul 2019 19:06:56: #1 total tags in treatment: 1456572 INFO @ Fri, 05 Jul 2019 19:06:56: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:06:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:06:56: #1 tags after filtering in treatment: 1074474 INFO @ Fri, 05 Jul 2019 19:06:56: #1 Redundant rate of treatment: 0.26 INFO @ Fri, 05 Jul 2019 19:06:56: #1 finished! INFO @ Fri, 05 Jul 2019 19:06:56: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:06:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:06:56: #2 number of paired peaks: 58 WARNING @ Fri, 05 Jul 2019 19:06:56: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:06:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.05_peaks.narrowPeakcut: : No such file or directory/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.20_peaks.narrowPeak : No such file or directory pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.05_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.10_*.xls’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585835/ERX585835.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。