Job ID = 2008243 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 7,907,748 reads read : 15,815,496 reads written : 15,815,496 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR629072.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:47 7907748 reads; of these: 7907748 (100.00%) were paired; of these: 290839 (3.68%) aligned concordantly 0 times 7132335 (90.19%) aligned concordantly exactly 1 time 484574 (6.13%) aligned concordantly >1 times ---- 290839 pairs aligned concordantly 0 times; of these: 119408 (41.06%) aligned discordantly 1 time ---- 171431 pairs aligned 0 times concordantly or discordantly; of these: 342862 mates make up the pairs; of these: 251374 (73.32%) aligned 0 times 70791 (20.65%) aligned exactly 1 time 20697 (6.04%) aligned >1 times 98.41% overall alignment rate Time searching: 00:25:48 Overall time: 00:25:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1491742 / 7721577 = 0.1932 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 18:42:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:42:44: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:42:44: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:42:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:42:44: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:42:44: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:43:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:43:00: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:43:00: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:43:06: 1000000 INFO @ Fri, 05 Jul 2019 18:43:06: 1000000 INFO @ Fri, 05 Jul 2019 18:43:10: 1000000 INFO @ Fri, 05 Jul 2019 18:43:14: 2000000 INFO @ Fri, 05 Jul 2019 18:43:24: 2000000 INFO @ Fri, 05 Jul 2019 18:43:35: 2000000 INFO @ Fri, 05 Jul 2019 18:43:37: 3000000 INFO @ Fri, 05 Jul 2019 18:43:39: 3000000 INFO @ Fri, 05 Jul 2019 18:43:45: 3000000 INFO @ Fri, 05 Jul 2019 18:43:45: 4000000 INFO @ Fri, 05 Jul 2019 18:43:51: 4000000 INFO @ Fri, 05 Jul 2019 18:43:57: 5000000 INFO @ Fri, 05 Jul 2019 18:43:58: 4000000 INFO @ Fri, 05 Jul 2019 18:44:00: 5000000 INFO @ Fri, 05 Jul 2019 18:44:05: 6000000 INFO @ Fri, 05 Jul 2019 18:44:07: 5000000 INFO @ Fri, 05 Jul 2019 18:44:08: 6000000 INFO @ Fri, 05 Jul 2019 18:44:12: 7000000 INFO @ Fri, 05 Jul 2019 18:44:16: 7000000 INFO @ Fri, 05 Jul 2019 18:44:17: 6000000 INFO @ Fri, 05 Jul 2019 18:44:20: 8000000 INFO @ Fri, 05 Jul 2019 18:44:25: 8000000 INFO @ Fri, 05 Jul 2019 18:44:26: 7000000 INFO @ Fri, 05 Jul 2019 18:44:28: 9000000 INFO @ Fri, 05 Jul 2019 18:44:33: 9000000 INFO @ Fri, 05 Jul 2019 18:44:38: 8000000 INFO @ Fri, 05 Jul 2019 18:44:38: 10000000 INFO @ Fri, 05 Jul 2019 18:44:42: 10000000 INFO @ Fri, 05 Jul 2019 18:44:50: 11000000 INFO @ Fri, 05 Jul 2019 18:44:51: 9000000 INFO @ Fri, 05 Jul 2019 18:44:54: 11000000 INFO @ Fri, 05 Jul 2019 18:44:58: 12000000 INFO @ Fri, 05 Jul 2019 18:45:03: 10000000 INFO @ Fri, 05 Jul 2019 18:45:06: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 18:45:06: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 18:45:06: #1 total tags in treatment: 6129238 INFO @ Fri, 05 Jul 2019 18:45:06: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:45:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:45:06: #1 tags after filtering in treatment: 3359084 INFO @ Fri, 05 Jul 2019 18:45:06: #1 Redundant rate of treatment: 0.45 INFO @ Fri, 05 Jul 2019 18:45:06: #1 finished! INFO @ Fri, 05 Jul 2019 18:45:06: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:45:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:45:06: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 18:45:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:45:06: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 18:45:07: 12000000 INFO @ Fri, 05 Jul 2019 18:45:12: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 18:45:12: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 18:45:12: #1 total tags in treatment: 6129238 INFO @ Fri, 05 Jul 2019 18:45:12: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:45:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:45:12: #1 tags after filtering in treatment: 3359084 INFO @ Fri, 05 Jul 2019 18:45:12: #1 Redundant rate of treatment: 0.45 INFO @ Fri, 05 Jul 2019 18:45:12: #1 finished! INFO @ Fri, 05 Jul 2019 18:45:12: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:45:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:45:12: 11000000 INFO @ Fri, 05 Jul 2019 18:45:12: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 18:45:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:45:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.10_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 18:45:21: 12000000 INFO @ Fri, 05 Jul 2019 18:45:28: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 18:45:28: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 18:45:28: #1 total tags in treatment: 6129238 INFO @ Fri, 05 Jul 2019 18:45:28: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:45:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:45:29: #1 tags after filtering in treatment: 3359084 INFO @ Fri, 05 Jul 2019 18:45:29: #1 Redundant rate of treatment: 0.45 INFO @ Fri, 05 Jul 2019 18:45:29: #1 finished! INFO @ Fri, 05 Jul 2019 18:45:29: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:45:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:45:29: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 18:45:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:45:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585767/ERX585767.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。