Job ID = 2008232 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 2,151,729 reads read : 4,303,458 reads written : 4,303,458 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:48 2151729 reads; of these: 2151729 (100.00%) were paired; of these: 426969 (19.84%) aligned concordantly 0 times 1512377 (70.29%) aligned concordantly exactly 1 time 212383 (9.87%) aligned concordantly >1 times ---- 426969 pairs aligned concordantly 0 times; of these: 92687 (21.71%) aligned discordantly 1 time ---- 334282 pairs aligned 0 times concordantly or discordantly; of these: 668564 mates make up the pairs; of these: 581688 (87.01%) aligned 0 times 41269 (6.17%) aligned exactly 1 time 45607 (6.82%) aligned >1 times 86.48% overall alignment rate Time searching: 00:02:48 Overall time: 00:02:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 184267 / 1806086 = 0.1020 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 18:02:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:02:02: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:02:02: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:02:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:02:03: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:02:03: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:02:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:02:04: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:02:04: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:02:11: 1000000 INFO @ Fri, 05 Jul 2019 18:02:15: 1000000 INFO @ Fri, 05 Jul 2019 18:02:16: 1000000 INFO @ Fri, 05 Jul 2019 18:02:19: 2000000 INFO @ Fri, 05 Jul 2019 18:02:26: 2000000 INFO @ Fri, 05 Jul 2019 18:02:27: 3000000 INFO @ Fri, 05 Jul 2019 18:02:27: 2000000 INFO @ Fri, 05 Jul 2019 18:02:30: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 18:02:30: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 18:02:30: #1 total tags in treatment: 1543567 INFO @ Fri, 05 Jul 2019 18:02:30: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:02:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:02:30: #1 tags after filtering in treatment: 1215219 INFO @ Fri, 05 Jul 2019 18:02:30: #1 Redundant rate of treatment: 0.21 INFO @ Fri, 05 Jul 2019 18:02:30: #1 finished! INFO @ Fri, 05 Jul 2019 18:02:30: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:02:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:02:30: #2 number of paired peaks: 25 WARNING @ Fri, 05 Jul 2019 18:02:30: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:02:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 18:02:38: 3000000 INFO @ Fri, 05 Jul 2019 18:02:39: 3000000 INFO @ Fri, 05 Jul 2019 18:02:42: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 18:02:42: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 18:02:42: #1 total tags in treatment: 1543567 INFO @ Fri, 05 Jul 2019 18:02:42: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:02:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:02:42: #1 tags after filtering in treatment: 1215219 INFO @ Fri, 05 Jul 2019 18:02:42: #1 Redundant rate of treatment: 0.21 INFO @ Fri, 05 Jul 2019 18:02:42: #1 finished! INFO @ Fri, 05 Jul 2019 18:02:42: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:02:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:02:42: #2 number of paired peaks: 25 WARNING @ Fri, 05 Jul 2019 18:02:42: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:02:42: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 18:02:43: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 18:02:43: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 18:02:43: #1 total tags in treatment: 1543567 INFO @ Fri, 05 Jul 2019 18:02:43: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:02:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:02:43: #1 tags after filtering in treatment: 1215219 INFO @ Fri, 05 Jul 2019 18:02:43: #1 Redundant rate of treatment: 0.21 INFO @ Fri, 05 Jul 2019 18:02:43: #1 finished! INFO @ Fri, 05 Jul 2019 18:02:43: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:02:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:02:43: #2 number of paired peaks: 25 WARNING @ Fri, 05 Jul 2019 18:02:43: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:02:43: Process for pairing-model is terminated! BedGraph に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.20_peaks.narrowPeak: No such file or directory BigWig に変換中... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585756/ERX585756.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。