Job ID = 2008214 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 23,543,823 reads read : 47,087,646 reads written : 47,087,646 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:52 23543823 reads; of these: 23543823 (100.00%) were paired; of these: 1826397 (7.76%) aligned concordantly 0 times 18753509 (79.65%) aligned concordantly exactly 1 time 2963917 (12.59%) aligned concordantly >1 times ---- 1826397 pairs aligned concordantly 0 times; of these: 563617 (30.86%) aligned discordantly 1 time ---- 1262780 pairs aligned 0 times concordantly or discordantly; of these: 2525560 mates make up the pairs; of these: 2192064 (86.80%) aligned 0 times 139897 (5.54%) aligned exactly 1 time 193599 (7.67%) aligned >1 times 95.34% overall alignment rate Time searching: 00:30:52 Overall time: 00:30:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5340622 / 22177502 = 0.2408 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:18:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:18:02: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:18:02: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:18:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:18:03: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:18:03: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:18:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:18:04: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:18:04: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:18:13: 1000000 INFO @ Fri, 05 Jul 2019 19:18:13: 1000000 INFO @ Fri, 05 Jul 2019 19:18:14: 1000000 INFO @ Fri, 05 Jul 2019 19:18:21: 2000000 INFO @ Fri, 05 Jul 2019 19:18:24: 2000000 INFO @ Fri, 05 Jul 2019 19:18:25: 2000000 INFO @ Fri, 05 Jul 2019 19:18:30: 3000000 INFO @ Fri, 05 Jul 2019 19:18:36: 3000000 INFO @ Fri, 05 Jul 2019 19:18:37: 3000000 INFO @ Fri, 05 Jul 2019 19:18:38: 4000000 INFO @ Fri, 05 Jul 2019 19:18:47: 5000000 INFO @ Fri, 05 Jul 2019 19:18:47: 4000000 INFO @ Fri, 05 Jul 2019 19:18:48: 4000000 INFO @ Fri, 05 Jul 2019 19:18:55: 6000000 INFO @ Fri, 05 Jul 2019 19:18:59: 5000000 INFO @ Fri, 05 Jul 2019 19:19:00: 5000000 INFO @ Fri, 05 Jul 2019 19:19:03: 7000000 INFO @ Fri, 05 Jul 2019 19:19:10: 6000000 INFO @ Fri, 05 Jul 2019 19:19:11: 6000000 INFO @ Fri, 05 Jul 2019 19:19:11: 8000000 INFO @ Fri, 05 Jul 2019 19:19:19: 9000000 INFO @ Fri, 05 Jul 2019 19:19:22: 7000000 INFO @ Fri, 05 Jul 2019 19:19:22: 7000000 INFO @ Fri, 05 Jul 2019 19:19:28: 10000000 INFO @ Fri, 05 Jul 2019 19:19:32: 8000000 INFO @ Fri, 05 Jul 2019 19:19:34: 8000000 INFO @ Fri, 05 Jul 2019 19:19:36: 11000000 INFO @ Fri, 05 Jul 2019 19:19:43: 9000000 INFO @ Fri, 05 Jul 2019 19:19:44: 12000000 INFO @ Fri, 05 Jul 2019 19:19:45: 9000000 INFO @ Fri, 05 Jul 2019 19:19:53: 13000000 INFO @ Fri, 05 Jul 2019 19:19:54: 10000000 INFO @ Fri, 05 Jul 2019 19:19:56: 10000000 INFO @ Fri, 05 Jul 2019 19:20:02: 14000000 INFO @ Fri, 05 Jul 2019 19:20:05: 11000000 INFO @ Fri, 05 Jul 2019 19:20:07: 11000000 INFO @ Fri, 05 Jul 2019 19:20:10: 15000000 INFO @ Fri, 05 Jul 2019 19:20:16: 12000000 INFO @ Fri, 05 Jul 2019 19:20:18: 16000000 INFO @ Fri, 05 Jul 2019 19:20:18: 12000000 INFO @ Fri, 05 Jul 2019 19:20:26: 17000000 INFO @ Fri, 05 Jul 2019 19:20:28: 13000000 INFO @ Fri, 05 Jul 2019 19:20:29: 13000000 INFO @ Fri, 05 Jul 2019 19:20:34: 18000000 INFO @ Fri, 05 Jul 2019 19:20:38: 14000000 INFO @ Fri, 05 Jul 2019 19:20:39: 14000000 INFO @ Fri, 05 Jul 2019 19:20:42: 19000000 INFO @ Fri, 05 Jul 2019 19:20:48: 15000000 INFO @ Fri, 05 Jul 2019 19:20:49: 15000000 INFO @ Fri, 05 Jul 2019 19:20:50: 20000000 INFO @ Fri, 05 Jul 2019 19:20:57: 21000000 INFO @ Fri, 05 Jul 2019 19:20:59: 16000000 INFO @ Fri, 05 Jul 2019 19:21:00: 16000000 INFO @ Fri, 05 Jul 2019 19:21:05: 22000000 INFO @ Fri, 05 Jul 2019 19:21:09: 17000000 INFO @ Fri, 05 Jul 2019 19:21:11: 17000000 INFO @ Fri, 05 Jul 2019 19:21:13: 23000000 INFO @ Fri, 05 Jul 2019 19:21:19: 18000000 INFO @ Fri, 05 Jul 2019 19:21:21: 24000000 INFO @ Fri, 05 Jul 2019 19:21:21: 18000000 INFO @ Fri, 05 Jul 2019 19:21:29: 25000000 INFO @ Fri, 05 Jul 2019 19:21:29: 19000000 INFO @ Fri, 05 Jul 2019 19:21:31: 19000000 INFO @ Fri, 05 Jul 2019 19:21:37: 26000000 INFO @ Fri, 05 Jul 2019 19:21:39: 20000000 INFO @ Fri, 05 Jul 2019 19:21:40: 20000000 INFO @ Fri, 05 Jul 2019 19:21:45: 27000000 INFO @ Fri, 05 Jul 2019 19:21:48: 21000000 INFO @ Fri, 05 Jul 2019 19:21:49: 21000000 INFO @ Fri, 05 Jul 2019 19:21:53: 28000000 INFO @ Fri, 05 Jul 2019 19:21:57: 22000000 INFO @ Fri, 05 Jul 2019 19:21:59: 22000000 INFO @ Fri, 05 Jul 2019 19:22:01: 29000000 INFO @ Fri, 05 Jul 2019 19:22:07: 23000000 INFO @ Fri, 05 Jul 2019 19:22:08: 23000000 INFO @ Fri, 05 Jul 2019 19:22:08: 30000000 INFO @ Fri, 05 Jul 2019 19:22:16: 24000000 INFO @ Fri, 05 Jul 2019 19:22:16: 31000000 INFO @ Fri, 05 Jul 2019 19:22:18: 24000000 INFO @ Fri, 05 Jul 2019 19:22:24: 32000000 INFO @ Fri, 05 Jul 2019 19:22:26: 25000000 INFO @ Fri, 05 Jul 2019 19:22:28: 25000000 INFO @ Fri, 05 Jul 2019 19:22:32: 33000000 INFO @ Fri, 05 Jul 2019 19:22:36: 26000000 INFO @ Fri, 05 Jul 2019 19:22:37: 26000000 INFO @ Fri, 05 Jul 2019 19:22:40: 34000000 INFO @ Fri, 05 Jul 2019 19:22:42: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 19:22:42: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 19:22:42: #1 total tags in treatment: 16405485 INFO @ Fri, 05 Jul 2019 19:22:42: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:22:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:22:42: #1 tags after filtering in treatment: 6815380 INFO @ Fri, 05 Jul 2019 19:22:42: #1 Redundant rate of treatment: 0.58 INFO @ Fri, 05 Jul 2019 19:22:42: #1 finished! INFO @ Fri, 05 Jul 2019 19:22:42: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:22:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:22:43: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:22:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:22:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:22:45: 27000000 INFO @ Fri, 05 Jul 2019 19:22:47: 27000000 INFO @ Fri, 05 Jul 2019 19:22:54: 28000000 INFO @ Fri, 05 Jul 2019 19:22:56: 28000000 INFO @ Fri, 05 Jul 2019 19:23:03: 29000000 INFO @ Fri, 05 Jul 2019 19:23:05: 29000000 INFO @ Fri, 05 Jul 2019 19:23:12: 30000000 INFO @ Fri, 05 Jul 2019 19:23:15: 30000000 INFO @ Fri, 05 Jul 2019 19:23:22: 31000000 INFO @ Fri, 05 Jul 2019 19:23:24: 31000000 INFO @ Fri, 05 Jul 2019 19:23:31: 32000000 INFO @ Fri, 05 Jul 2019 19:23:33: 32000000 INFO @ Fri, 05 Jul 2019 19:23:40: 33000000 INFO @ Fri, 05 Jul 2019 19:23:43: 33000000 INFO @ Fri, 05 Jul 2019 19:23:50: 34000000 INFO @ Fri, 05 Jul 2019 19:23:52: 34000000 INFO @ Fri, 05 Jul 2019 19:23:52: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 19:23:52: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 19:23:52: #1 total tags in treatment: 16405485 INFO @ Fri, 05 Jul 2019 19:23:52: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:23:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:23:52: #1 tags after filtering in treatment: 6815380 INFO @ Fri, 05 Jul 2019 19:23:52: #1 Redundant rate of treatment: 0.58 INFO @ Fri, 05 Jul 2019 19:23:52: #1 finished! INFO @ Fri, 05 Jul 2019 19:23:52: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:23:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:23:53: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:23:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:23:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:23:54: #1 tag size is determined as 94 bps INFO @ Fri, 05 Jul 2019 19:23:54: #1 tag size = 94 INFO @ Fri, 05 Jul 2019 19:23:54: #1 total tags in treatment: 16405485 INFO @ Fri, 05 Jul 2019 19:23:54: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:23:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:23:54: #1 tags after filtering in treatment: 6815380 INFO @ Fri, 05 Jul 2019 19:23:54: #1 Redundant rate of treatment: 0.58 INFO @ Fri, 05 Jul 2019 19:23:54: #1 finished! INFO @ Fri, 05 Jul 2019 19:23:54: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:23:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:23:55: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:23:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:23:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 30 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585743/ERX585743.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。