Job ID = 2008191 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 18,221,519 reads read : 36,443,038 reads written : 36,443,038 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR628992.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:32 18221519 reads; of these: 18221519 (100.00%) were paired; of these: 927706 (5.09%) aligned concordantly 0 times 16055638 (88.11%) aligned concordantly exactly 1 time 1238175 (6.80%) aligned concordantly >1 times ---- 927706 pairs aligned concordantly 0 times; of these: 104575 (11.27%) aligned discordantly 1 time ---- 823131 pairs aligned 0 times concordantly or discordantly; of these: 1646262 mates make up the pairs; of these: 1542228 (93.68%) aligned 0 times 75690 (4.60%) aligned exactly 1 time 28344 (1.72%) aligned >1 times 95.77% overall alignment rate Time searching: 00:17:32 Overall time: 00:17:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 15722802 / 17385204 = 0.9044 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 18:13:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:13:36: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:13:36: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:13:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:13:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:13:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:13:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:13:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:13:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:13:44: 1000000 INFO @ Fri, 05 Jul 2019 18:13:47: 1000000 INFO @ Fri, 05 Jul 2019 18:13:48: 1000000 INFO @ Fri, 05 Jul 2019 18:13:50: 2000000 INFO @ Fri, 05 Jul 2019 18:13:57: 3000000 INFO @ Fri, 05 Jul 2019 18:13:57: 2000000 INFO @ Fri, 05 Jul 2019 18:13:58: 2000000 INFO @ Fri, 05 Jul 2019 18:14:00: #1 tag size is determined as 44 bps INFO @ Fri, 05 Jul 2019 18:14:00: #1 tag size = 44 INFO @ Fri, 05 Jul 2019 18:14:00: #1 total tags in treatment: 1599457 INFO @ Fri, 05 Jul 2019 18:14:00: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:14:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:14:00: #1 tags after filtering in treatment: 1171759 INFO @ Fri, 05 Jul 2019 18:14:00: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 05 Jul 2019 18:14:00: #1 finished! INFO @ Fri, 05 Jul 2019 18:14:00: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:14:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:14:00: #2 number of paired peaks: 14 WARNING @ Fri, 05 Jul 2019 18:14:00: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:14:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 18:14:06: 3000000 INFO @ Fri, 05 Jul 2019 18:14:07: 3000000 INFO @ Fri, 05 Jul 2019 18:14:10: #1 tag size is determined as 44 bps INFO @ Fri, 05 Jul 2019 18:14:10: #1 tag size = 44 INFO @ Fri, 05 Jul 2019 18:14:10: #1 total tags in treatment: 1599457 INFO @ Fri, 05 Jul 2019 18:14:10: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:14:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:14:10: #1 tags after filtering in treatment: 1171759 INFO @ Fri, 05 Jul 2019 18:14:10: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 05 Jul 2019 18:14:10: #1 finished! INFO @ Fri, 05 Jul 2019 18:14:10: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:14:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:14:11: #2 number of paired peaks: 14 WARNING @ Fri, 05 Jul 2019 18:14:11: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:14:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 18:14:11: #1 tag size is determined as 44 bps INFO @ Fri, 05 Jul 2019 18:14:11: #1 tag size = 44 INFO @ Fri, 05 Jul 2019 18:14:11: #1 total tags in treatment: 1599457 INFO @ Fri, 05 Jul 2019 18:14:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:14:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:14:11: #1 tags after filtering in treatment: 1171759 INFO @ Fri, 05 Jul 2019 18:14:11: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 05 Jul 2019 18:14:11: #1 finished! INFO @ Fri, 05 Jul 2019 18:14:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:14:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:14:11: #2 number of paired peaks: 14 WARNING @ Fri, 05 Jul 2019 18:14:11: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:14:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX585726/ERX585726.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。