Job ID = 2008153 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 16,925,631 reads read : 33,851,262 reads written : 33,851,262 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:21 16925631 reads; of these: 16925631 (100.00%) were paired; of these: 1063618 (6.28%) aligned concordantly 0 times 12690824 (74.98%) aligned concordantly exactly 1 time 3171189 (18.74%) aligned concordantly >1 times ---- 1063618 pairs aligned concordantly 0 times; of these: 9697 (0.91%) aligned discordantly 1 time ---- 1053921 pairs aligned 0 times concordantly or discordantly; of these: 2107842 mates make up the pairs; of these: 2068079 (98.11%) aligned 0 times 22964 (1.09%) aligned exactly 1 time 16799 (0.80%) aligned >1 times 93.89% overall alignment rate Time searching: 00:13:21 Overall time: 00:13:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1248754 / 15864216 = 0.0787 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 18:16:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:16:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:16:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:16:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:16:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:16:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 18:16:23: #1 read tag files... INFO @ Fri, 05 Jul 2019 18:16:23: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 18:16:28: 1000000 INFO @ Fri, 05 Jul 2019 18:16:29: 1000000 INFO @ Fri, 05 Jul 2019 18:16:30: 1000000 INFO @ Fri, 05 Jul 2019 18:16:34: 2000000 INFO @ Fri, 05 Jul 2019 18:16:35: 2000000 INFO @ Fri, 05 Jul 2019 18:16:38: 2000000 INFO @ Fri, 05 Jul 2019 18:16:41: 3000000 INFO @ Fri, 05 Jul 2019 18:16:42: 3000000 INFO @ Fri, 05 Jul 2019 18:16:46: 3000000 INFO @ Fri, 05 Jul 2019 18:16:48: 4000000 INFO @ Fri, 05 Jul 2019 18:16:48: 4000000 INFO @ Fri, 05 Jul 2019 18:16:54: 4000000 INFO @ Fri, 05 Jul 2019 18:16:54: 5000000 INFO @ Fri, 05 Jul 2019 18:16:55: 5000000 INFO @ Fri, 05 Jul 2019 18:17:01: 6000000 INFO @ Fri, 05 Jul 2019 18:17:01: 5000000 INFO @ Fri, 05 Jul 2019 18:17:02: 6000000 INFO @ Fri, 05 Jul 2019 18:17:07: 7000000 INFO @ Fri, 05 Jul 2019 18:17:08: 7000000 INFO @ Fri, 05 Jul 2019 18:17:09: 6000000 INFO @ Fri, 05 Jul 2019 18:17:14: 8000000 INFO @ Fri, 05 Jul 2019 18:17:15: 8000000 INFO @ Fri, 05 Jul 2019 18:17:17: 7000000 INFO @ Fri, 05 Jul 2019 18:17:21: 9000000 INFO @ Fri, 05 Jul 2019 18:17:22: 9000000 INFO @ Fri, 05 Jul 2019 18:17:24: 8000000 INFO @ Fri, 05 Jul 2019 18:17:27: 10000000 INFO @ Fri, 05 Jul 2019 18:17:28: 10000000 INFO @ Fri, 05 Jul 2019 18:17:32: 9000000 INFO @ Fri, 05 Jul 2019 18:17:34: 11000000 INFO @ Fri, 05 Jul 2019 18:17:35: 11000000 INFO @ Fri, 05 Jul 2019 18:17:40: 10000000 INFO @ Fri, 05 Jul 2019 18:17:40: 12000000 INFO @ Fri, 05 Jul 2019 18:17:41: 12000000 INFO @ Fri, 05 Jul 2019 18:17:47: 13000000 INFO @ Fri, 05 Jul 2019 18:17:47: 11000000 INFO @ Fri, 05 Jul 2019 18:17:48: 13000000 INFO @ Fri, 05 Jul 2019 18:17:54: 14000000 INFO @ Fri, 05 Jul 2019 18:17:54: 14000000 INFO @ Fri, 05 Jul 2019 18:17:55: 12000000 INFO @ Fri, 05 Jul 2019 18:18:00: 15000000 INFO @ Fri, 05 Jul 2019 18:18:01: 15000000 INFO @ Fri, 05 Jul 2019 18:18:02: 13000000 INFO @ Fri, 05 Jul 2019 18:18:07: 16000000 INFO @ Fri, 05 Jul 2019 18:18:08: 16000000 INFO @ Fri, 05 Jul 2019 18:18:10: 14000000 INFO @ Fri, 05 Jul 2019 18:18:13: 17000000 INFO @ Fri, 05 Jul 2019 18:18:14: 17000000 INFO @ Fri, 05 Jul 2019 18:18:18: 15000000 INFO @ Fri, 05 Jul 2019 18:18:20: 18000000 INFO @ Fri, 05 Jul 2019 18:18:21: 18000000 INFO @ Fri, 05 Jul 2019 18:18:25: 16000000 INFO @ Fri, 05 Jul 2019 18:18:26: 19000000 INFO @ Fri, 05 Jul 2019 18:18:27: 19000000 INFO @ Fri, 05 Jul 2019 18:18:33: 20000000 INFO @ Fri, 05 Jul 2019 18:18:33: 17000000 INFO @ Fri, 05 Jul 2019 18:18:34: 20000000 INFO @ Fri, 05 Jul 2019 18:18:39: 21000000 INFO @ Fri, 05 Jul 2019 18:18:40: 21000000 INFO @ Fri, 05 Jul 2019 18:18:41: 18000000 INFO @ Fri, 05 Jul 2019 18:18:46: 22000000 INFO @ Fri, 05 Jul 2019 18:18:49: 22000000 INFO @ Fri, 05 Jul 2019 18:18:49: 19000000 INFO @ Fri, 05 Jul 2019 18:18:53: 23000000 INFO @ Fri, 05 Jul 2019 18:18:55: 23000000 INFO @ Fri, 05 Jul 2019 18:18:57: 20000000 INFO @ Fri, 05 Jul 2019 18:18:59: 24000000 INFO @ Fri, 05 Jul 2019 18:19:02: 24000000 INFO @ Fri, 05 Jul 2019 18:19:04: 21000000 INFO @ Fri, 05 Jul 2019 18:19:06: 25000000 INFO @ Fri, 05 Jul 2019 18:19:08: 25000000 INFO @ Fri, 05 Jul 2019 18:19:12: 22000000 INFO @ Fri, 05 Jul 2019 18:19:12: 26000000 INFO @ Fri, 05 Jul 2019 18:19:15: 26000000 INFO @ Fri, 05 Jul 2019 18:19:19: 27000000 INFO @ Fri, 05 Jul 2019 18:19:20: 23000000 INFO @ Fri, 05 Jul 2019 18:19:21: 27000000 INFO @ Fri, 05 Jul 2019 18:19:25: 28000000 INFO @ Fri, 05 Jul 2019 18:19:27: 24000000 INFO @ Fri, 05 Jul 2019 18:19:28: 28000000 INFO @ Fri, 05 Jul 2019 18:19:32: 29000000 INFO @ Fri, 05 Jul 2019 18:19:34: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 18:19:34: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 18:19:34: #1 total tags in treatment: 14613349 INFO @ Fri, 05 Jul 2019 18:19:34: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:19:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:19:34: #1 tags after filtering in treatment: 9287787 INFO @ Fri, 05 Jul 2019 18:19:34: #1 Redundant rate of treatment: 0.36 INFO @ Fri, 05 Jul 2019 18:19:34: #1 finished! INFO @ Fri, 05 Jul 2019 18:19:34: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:19:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:19:34: 29000000 INFO @ Fri, 05 Jul 2019 18:19:35: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 18:19:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:19:35: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 18:19:35: 25000000 cut: /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 18:19:37: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 18:19:37: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 18:19:37: #1 total tags in treatment: 14613349 INFO @ Fri, 05 Jul 2019 18:19:37: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:19:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:19:37: #1 tags after filtering in treatment: 9287787 INFO @ Fri, 05 Jul 2019 18:19:37: #1 Redundant rate of treatment: 0.36 INFO @ Fri, 05 Jul 2019 18:19:37: #1 finished! INFO @ Fri, 05 Jul 2019 18:19:37: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:19:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:19:38: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 18:19:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:19:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 18:19:42: 26000000 INFO @ Fri, 05 Jul 2019 18:19:49: 27000000 INFO @ Fri, 05 Jul 2019 18:19:55: 28000000 INFO @ Fri, 05 Jul 2019 18:20:02: 29000000 INFO @ Fri, 05 Jul 2019 18:20:04: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 18:20:04: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 18:20:04: #1 total tags in treatment: 14613349 INFO @ Fri, 05 Jul 2019 18:20:04: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 18:20:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 18:20:04: #1 tags after filtering in treatment: 9287787 INFO @ Fri, 05 Jul 2019 18:20:04: #1 Redundant rate of treatment: 0.36 INFO @ Fri, 05 Jul 2019 18:20:04: #1 finished! INFO @ Fri, 05 Jul 2019 18:20:04: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 18:20:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 18:20:05: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 18:20:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 18:20:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX516017/ERX516017.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。