Job ID = 2008077 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 1,870,954 reads read : 3,741,908 reads written : 3,741,908 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:09 1870954 reads; of these: 1870954 (100.00%) were paired; of these: 284288 (15.19%) aligned concordantly 0 times 1464783 (78.29%) aligned concordantly exactly 1 time 121883 (6.51%) aligned concordantly >1 times ---- 284288 pairs aligned concordantly 0 times; of these: 171887 (60.46%) aligned discordantly 1 time ---- 112401 pairs aligned 0 times concordantly or discordantly; of these: 224802 mates make up the pairs; of these: 157955 (70.26%) aligned 0 times 33264 (14.80%) aligned exactly 1 time 33583 (14.94%) aligned >1 times 95.78% overall alignment rate Time searching: 00:02:09 Overall time: 00:02:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 14359 / 1753354 = 0.0082 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 17:32:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:32:29: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:32:29: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:32:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:32:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:32:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:32:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:32:31: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:32:31: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:32:40: 1000000 INFO @ Fri, 05 Jul 2019 17:32:41: 1000000 INFO @ Fri, 05 Jul 2019 17:32:41: 1000000 INFO @ Fri, 05 Jul 2019 17:32:50: 2000000 INFO @ Fri, 05 Jul 2019 17:32:51: 2000000 INFO @ Fri, 05 Jul 2019 17:32:53: 2000000 INFO @ Fri, 05 Jul 2019 17:33:00: 3000000 INFO @ Fri, 05 Jul 2019 17:33:02: 3000000 INFO @ Fri, 05 Jul 2019 17:33:04: 3000000 INFO @ Fri, 05 Jul 2019 17:33:05: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:33:05: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:33:05: #1 total tags in treatment: 1573062 INFO @ Fri, 05 Jul 2019 17:33:05: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:33:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:33:05: #1 tags after filtering in treatment: 1516350 INFO @ Fri, 05 Jul 2019 17:33:05: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 05 Jul 2019 17:33:05: #1 finished! INFO @ Fri, 05 Jul 2019 17:33:05: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:33:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 17:33:05: #2 number of paired peaks: 3 WARNING @ Fri, 05 Jul 2019 17:33:05: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 17:33:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.20_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 17:33:08: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:33:08: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:33:08: #1 total tags in treatment: 1573062 INFO @ Fri, 05 Jul 2019 17:33:08: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:33:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:33:08: #1 tags after filtering in treatment: 1516350 INFO @ Fri, 05 Jul 2019 17:33:08: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 05 Jul 2019 17:33:08: #1 finished! INFO @ Fri, 05 Jul 2019 17:33:08: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:33:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 17:33:08: #2 number of paired peaks: 3 WARNING @ Fri, 05 Jul 2019 17:33:08: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 17:33:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.05_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 17:33:09: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:33:09: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:33:09: #1 total tags in treatment: 1573062 INFO @ Fri, 05 Jul 2019 17:33:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:33:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:33:09: #1 tags after filtering in treatment: 1516350 INFO @ Fri, 05 Jul 2019 17:33:09: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 05 Jul 2019 17:33:09: #1 finished! INFO @ Fri, 05 Jul 2019 17:33:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:33:09: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.05_*.xls’: No such file or directory INFO @ Fri, 05 Jul 2019 17:33:10: #2 number of paired peaks: 3 rm: WARNING @ Fri, 05 Jul 2019 17:33:10: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.05_peaks.narrowPeak’: No such file or directory WARNING @ Fri, 05 Jul 2019 17:33:10: Process for pairing-model is terminated! CompletedMACS2peakCalling cut: /home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2852 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471856/ERX471856.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。