Job ID = 2008064 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 1,702,003 reads read : 3,404,006 reads written : 3,404,006 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:00 1702003 reads; of these: 1702003 (100.00%) were paired; of these: 173215 (10.18%) aligned concordantly 0 times 1413118 (83.03%) aligned concordantly exactly 1 time 115670 (6.80%) aligned concordantly >1 times ---- 173215 pairs aligned concordantly 0 times; of these: 93064 (53.73%) aligned discordantly 1 time ---- 80151 pairs aligned 0 times concordantly or discordantly; of these: 160302 mates make up the pairs; of these: 108893 (67.93%) aligned 0 times 31423 (19.60%) aligned exactly 1 time 19986 (12.47%) aligned >1 times 96.80% overall alignment rate Time searching: 00:02:00 Overall time: 00:02:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 35378 / 1601920 = 0.0221 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 17:30:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:30:12: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:30:12: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:30:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:30:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:30:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:30:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:30:14: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:30:14: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:30:25: 1000000 INFO @ Fri, 05 Jul 2019 17:30:28: 1000000 INFO @ Fri, 05 Jul 2019 17:30:29: 1000000 INFO @ Fri, 05 Jul 2019 17:30:37: 2000000 INFO @ Fri, 05 Jul 2019 17:30:44: 2000000 INFO @ Fri, 05 Jul 2019 17:30:44: 2000000 INFO @ Fri, 05 Jul 2019 17:30:47: 3000000 INFO @ Fri, 05 Jul 2019 17:30:49: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:30:49: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:30:49: #1 total tags in treatment: 1494294 INFO @ Fri, 05 Jul 2019 17:30:49: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:30:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:30:49: #1 tags after filtering in treatment: 1371189 INFO @ Fri, 05 Jul 2019 17:30:49: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 05 Jul 2019 17:30:49: #1 finished! INFO @ Fri, 05 Jul 2019 17:30:49: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:30:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 17:30:49: #2 number of paired peaks: 316 WARNING @ Fri, 05 Jul 2019 17:30:49: Fewer paired peaks (316) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 316 pairs to build model! INFO @ Fri, 05 Jul 2019 17:30:49: start model_add_line... INFO @ Fri, 05 Jul 2019 17:30:49: start X-correlation... INFO @ Fri, 05 Jul 2019 17:30:49: end of X-cor INFO @ Fri, 05 Jul 2019 17:30:49: #2 finished! INFO @ Fri, 05 Jul 2019 17:30:49: #2 predicted fragment length is 156 bps INFO @ Fri, 05 Jul 2019 17:30:49: #2 alternative fragment length(s) may be 156,168,183 bps INFO @ Fri, 05 Jul 2019 17:30:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.05_model.r WARNING @ Fri, 05 Jul 2019 17:30:49: #2 Since the d (156) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 17:30:49: #2 You may need to consider one of the other alternative d(s): 156,168,183 WARNING @ Fri, 05 Jul 2019 17:30:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 17:30:49: #3 Call peaks... INFO @ Fri, 05 Jul 2019 17:30:49: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 17:30:54: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 17:30:56: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.05_peaks.xls INFO @ Fri, 05 Jul 2019 17:30:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 17:30:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.05_summits.bed INFO @ Fri, 05 Jul 2019 17:30:56: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (638 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 17:30:59: 3000000 INFO @ Fri, 05 Jul 2019 17:30:59: 3000000 INFO @ Fri, 05 Jul 2019 17:31:03: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:31:03: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:31:03: #1 total tags in treatment: 1494294 INFO @ Fri, 05 Jul 2019 17:31:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:31:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:31:03: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:31:03: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:31:03: #1 total tags in treatment: 1494294 INFO @ Fri, 05 Jul 2019 17:31:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:31:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:31:03: #1 tags after filtering in treatment: 1371189 INFO @ Fri, 05 Jul 2019 17:31:03: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 05 Jul 2019 17:31:03: #1 finished! INFO @ Fri, 05 Jul 2019 17:31:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:31:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 17:31:03: #1 tags after filtering in treatment: 1371189 INFO @ Fri, 05 Jul 2019 17:31:03: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 05 Jul 2019 17:31:03: #1 finished! INFO @ Fri, 05 Jul 2019 17:31:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:31:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 17:31:03: #2 number of paired peaks: 316 WARNING @ Fri, 05 Jul 2019 17:31:03: Fewer paired peaks (316) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 316 pairs to build model! INFO @ Fri, 05 Jul 2019 17:31:03: start model_add_line... INFO @ Fri, 05 Jul 2019 17:31:03: #2 number of paired peaks: 316 WARNING @ Fri, 05 Jul 2019 17:31:03: Fewer paired peaks (316) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 316 pairs to build model! INFO @ Fri, 05 Jul 2019 17:31:03: start model_add_line... INFO @ Fri, 05 Jul 2019 17:31:03: start X-correlation... INFO @ Fri, 05 Jul 2019 17:31:03: end of X-cor INFO @ Fri, 05 Jul 2019 17:31:03: #2 finished! INFO @ Fri, 05 Jul 2019 17:31:03: #2 predicted fragment length is 156 bps INFO @ Fri, 05 Jul 2019 17:31:03: #2 alternative fragment length(s) may be 156,168,183 bps INFO @ Fri, 05 Jul 2019 17:31:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.10_model.r INFO @ Fri, 05 Jul 2019 17:31:03: start X-correlation... WARNING @ Fri, 05 Jul 2019 17:31:03: #2 Since the d (156) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 17:31:03: #2 You may need to consider one of the other alternative d(s): 156,168,183 WARNING @ Fri, 05 Jul 2019 17:31:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 17:31:03: #3 Call peaks... INFO @ Fri, 05 Jul 2019 17:31:03: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 17:31:03: end of X-cor INFO @ Fri, 05 Jul 2019 17:31:03: #2 finished! INFO @ Fri, 05 Jul 2019 17:31:03: #2 predicted fragment length is 156 bps INFO @ Fri, 05 Jul 2019 17:31:03: #2 alternative fragment length(s) may be 156,168,183 bps INFO @ Fri, 05 Jul 2019 17:31:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.20_model.r WARNING @ Fri, 05 Jul 2019 17:31:03: #2 Since the d (156) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 17:31:03: #2 You may need to consider one of the other alternative d(s): 156,168,183 WARNING @ Fri, 05 Jul 2019 17:31:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 17:31:03: #3 Call peaks... INFO @ Fri, 05 Jul 2019 17:31:03: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 17:31:07: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 17:31:07: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 17:31:09: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.20_peaks.xls INFO @ Fri, 05 Jul 2019 17:31:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 17:31:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.20_summits.bed INFO @ Fri, 05 Jul 2019 17:31:09: Done! INFO @ Fri, 05 Jul 2019 17:31:09: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.10_peaks.xls INFO @ Fri, 05 Jul 2019 17:31:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 17:31:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX471847/ERX471847.10_summits.bed INFO @ Fri, 05 Jul 2019 17:31:09: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (267 records, 4 fields): 3 millis pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (416 records, 4 fields): 5 millis CompletedMACS2peakCalling CompletedMACS2peakCalling BigWig に変換しました。