Job ID = 2007967 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 1,695,590 reads read : 3,391,180 reads written : 3,391,180 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:02 1695590 reads; of these: 1695590 (100.00%) were paired; of these: 117757 (6.94%) aligned concordantly 0 times 1447223 (85.35%) aligned concordantly exactly 1 time 130610 (7.70%) aligned concordantly >1 times ---- 117757 pairs aligned concordantly 0 times; of these: 54961 (46.67%) aligned discordantly 1 time ---- 62796 pairs aligned 0 times concordantly or discordantly; of these: 125592 mates make up the pairs; of these: 95630 (76.14%) aligned 0 times 17488 (13.92%) aligned exactly 1 time 12474 (9.93%) aligned >1 times 97.18% overall alignment rate Time searching: 00:02:02 Overall time: 00:02:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 43075 / 1628358 = 0.0265 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 17:03:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:03:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:03:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:03:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:03:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:03:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:03:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 17:03:35: #1 read tag files... INFO @ Fri, 05 Jul 2019 17:03:35: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 17:03:42: 1000000 INFO @ Fri, 05 Jul 2019 17:03:43: 1000000 INFO @ Fri, 05 Jul 2019 17:03:44: 1000000 INFO @ Fri, 05 Jul 2019 17:03:51: 2000000 INFO @ Fri, 05 Jul 2019 17:03:52: 2000000 INFO @ Fri, 05 Jul 2019 17:03:53: 2000000 INFO @ Fri, 05 Jul 2019 17:03:59: 3000000 INFO @ Fri, 05 Jul 2019 17:04:01: 3000000 INFO @ Fri, 05 Jul 2019 17:04:01: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:04:01: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:04:01: #1 total tags in treatment: 1535401 INFO @ Fri, 05 Jul 2019 17:04:01: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:04:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:04:01: #1 tags after filtering in treatment: 1412080 INFO @ Fri, 05 Jul 2019 17:04:01: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 05 Jul 2019 17:04:01: #1 finished! INFO @ Fri, 05 Jul 2019 17:04:01: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:04:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 17:04:01: #2 number of paired peaks: 210 WARNING @ Fri, 05 Jul 2019 17:04:01: Fewer paired peaks (210) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 210 pairs to build model! INFO @ Fri, 05 Jul 2019 17:04:01: start model_add_line... INFO @ Fri, 05 Jul 2019 17:04:01: start X-correlation... INFO @ Fri, 05 Jul 2019 17:04:01: end of X-cor INFO @ Fri, 05 Jul 2019 17:04:01: #2 finished! INFO @ Fri, 05 Jul 2019 17:04:01: #2 predicted fragment length is 144 bps INFO @ Fri, 05 Jul 2019 17:04:01: #2 alternative fragment length(s) may be 144 bps INFO @ Fri, 05 Jul 2019 17:04:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.05_model.r WARNING @ Fri, 05 Jul 2019 17:04:01: #2 Since the d (144) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 17:04:01: #2 You may need to consider one of the other alternative d(s): 144 WARNING @ Fri, 05 Jul 2019 17:04:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 17:04:01: #3 Call peaks... INFO @ Fri, 05 Jul 2019 17:04:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 17:04:02: 3000000 INFO @ Fri, 05 Jul 2019 17:04:03: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:04:03: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:04:03: #1 total tags in treatment: 1535401 INFO @ Fri, 05 Jul 2019 17:04:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:04:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:04:03: #1 tags after filtering in treatment: 1412080 INFO @ Fri, 05 Jul 2019 17:04:03: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 05 Jul 2019 17:04:03: #1 finished! INFO @ Fri, 05 Jul 2019 17:04:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:04:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 17:04:03: #2 number of paired peaks: 210 WARNING @ Fri, 05 Jul 2019 17:04:03: Fewer paired peaks (210) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 210 pairs to build model! INFO @ Fri, 05 Jul 2019 17:04:03: start model_add_line... INFO @ Fri, 05 Jul 2019 17:04:03: start X-correlation... INFO @ Fri, 05 Jul 2019 17:04:03: end of X-cor INFO @ Fri, 05 Jul 2019 17:04:03: #2 finished! INFO @ Fri, 05 Jul 2019 17:04:03: #2 predicted fragment length is 144 bps INFO @ Fri, 05 Jul 2019 17:04:03: #2 alternative fragment length(s) may be 144 bps INFO @ Fri, 05 Jul 2019 17:04:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.10_model.r WARNING @ Fri, 05 Jul 2019 17:04:03: #2 Since the d (144) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 17:04:03: #2 You may need to consider one of the other alternative d(s): 144 WARNING @ Fri, 05 Jul 2019 17:04:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 17:04:03: #3 Call peaks... INFO @ Fri, 05 Jul 2019 17:04:03: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 17:04:04: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 17:04:04: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 17:04:04: #1 total tags in treatment: 1535401 INFO @ Fri, 05 Jul 2019 17:04:04: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 17:04:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 17:04:04: #1 tags after filtering in treatment: 1412080 INFO @ Fri, 05 Jul 2019 17:04:04: #1 Redundant rate of treatment: 0.08 INFO @ Fri, 05 Jul 2019 17:04:04: #1 finished! INFO @ Fri, 05 Jul 2019 17:04:04: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 17:04:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 17:04:04: #2 number of paired peaks: 210 WARNING @ Fri, 05 Jul 2019 17:04:04: Fewer paired peaks (210) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 210 pairs to build model! INFO @ Fri, 05 Jul 2019 17:04:04: start model_add_line... INFO @ Fri, 05 Jul 2019 17:04:04: start X-correlation... INFO @ Fri, 05 Jul 2019 17:04:04: end of X-cor INFO @ Fri, 05 Jul 2019 17:04:04: #2 finished! INFO @ Fri, 05 Jul 2019 17:04:04: #2 predicted fragment length is 144 bps INFO @ Fri, 05 Jul 2019 17:04:04: #2 alternative fragment length(s) may be 144 bps INFO @ Fri, 05 Jul 2019 17:04:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.20_model.r WARNING @ Fri, 05 Jul 2019 17:04:04: #2 Since the d (144) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 17:04:04: #2 You may need to consider one of the other alternative d(s): 144 WARNING @ Fri, 05 Jul 2019 17:04:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 17:04:04: #3 Call peaks... INFO @ Fri, 05 Jul 2019 17:04:04: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 17:04:06: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 17:04:08: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 17:04:08: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.05_peaks.xls INFO @ Fri, 05 Jul 2019 17:04:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 17:04:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.05_summits.bed INFO @ Fri, 05 Jul 2019 17:04:08: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (715 records, 4 fields): 13 millis INFO @ Fri, 05 Jul 2019 17:04:09: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 17:04:10: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.10_peaks.xls INFO @ Fri, 05 Jul 2019 17:04:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 17:04:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.10_summits.bed INFO @ Fri, 05 Jul 2019 17:04:10: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (461 records, 4 fields): 4 millis INFO @ Fri, 05 Jul 2019 17:04:11: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.20_peaks.xls INFO @ Fri, 05 Jul 2019 17:04:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 17:04:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX471758/ERX471758.20_summits.bed INFO @ Fri, 05 Jul 2019 17:04:11: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (237 records, 4 fields): 3 millis CompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。