Job ID = 2007951 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-07-05T07:40:38 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T07:40:38 fasterq-dump.2.9.6 err: connection busy while validating within network system module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/sos/sra-pub-run-2/ERR506387/ERR506387.1' 2019-07-05T07:40:38 fasterq-dump.2.9.6 err: cmn_iter.c cmn_get_db_type().VDBManagerOpenDBRead( 'ERR506387' ) -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T07:40:38 fasterq-dump.2.9.6 err: invalid accession 'ERR506387' 2019-07-05T07:40:53 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T07:40:53 fasterq-dump.2.9.6 err: connection busy while validating within network system module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/sos/sra-pub-run-2/ERR506387/ERR506387.1' 2019-07-05T07:40:53 fasterq-dump.2.9.6 err: invalid accession 'ERR506387' 2019-07-05T07:41:08 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T07:41:08 fasterq-dump.2.9.6 err: connection busy while validating within network system module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/sos/sra-pub-run-2/ERR506387/ERR506387.1' 2019-07-05T07:41:08 fasterq-dump.2.9.6 err: invalid accession 'ERR506387' 2019-07-05T07:41:22 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T07:41:22 fasterq-dump.2.9.6 err: connection busy while validating within network system module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/sos/sra-pub-run-2/ERR506387/ERR506387.1' 2019-07-05T07:41:22 fasterq-dump.2.9.6 err: invalid accession 'ERR506387' spots read : 1,886,880 reads read : 3,773,760 reads written : 3,773,760 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:31 1886880 reads; of these: 1886880 (100.00%) were paired; of these: 291127 (15.43%) aligned concordantly 0 times 1464150 (77.60%) aligned concordantly exactly 1 time 131603 (6.97%) aligned concordantly >1 times ---- 291127 pairs aligned concordantly 0 times; of these: 180881 (62.13%) aligned discordantly 1 time ---- 110246 pairs aligned 0 times concordantly or discordantly; of these: 220492 mates make up the pairs; of these: 145767 (66.11%) aligned 0 times 36522 (16.56%) aligned exactly 1 time 38203 (17.33%) aligned >1 times 96.14% overall alignment rate Time searching: 00:02:31 Overall time: 00:02:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 9958 / 1764839 = 0.0056 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 16:54:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:54:42: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:54:42: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:54:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:54:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:54:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:54:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:54:44: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:54:44: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:54:52: 1000000 INFO @ Fri, 05 Jul 2019 16:54:54: 1000000 INFO @ Fri, 05 Jul 2019 16:54:56: 1000000 INFO @ Fri, 05 Jul 2019 16:55:01: 2000000 INFO @ Fri, 05 Jul 2019 16:55:06: 2000000 INFO @ Fri, 05 Jul 2019 16:55:08: 2000000 INFO @ Fri, 05 Jul 2019 16:55:10: 3000000 INFO @ Fri, 05 Jul 2019 16:55:15: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 16:55:15: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 16:55:15: #1 total tags in treatment: 1586383 INFO @ Fri, 05 Jul 2019 16:55:15: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:55:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:55:15: #1 tags after filtering in treatment: 1525458 INFO @ Fri, 05 Jul 2019 16:55:15: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 05 Jul 2019 16:55:15: #1 finished! INFO @ Fri, 05 Jul 2019 16:55:15: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:55:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:55:15: #2 number of paired peaks: 10 WARNING @ Fri, 05 Jul 2019 16:55:15: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:55:17: 3000000 INFO @ Fri, 05 Jul 2019 16:55:21: 3000000 INFO @ Fri, 05 Jul 2019 16:55:24: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 16:55:24: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 16:55:24: #1 total tags in treatment: 1586383 INFO @ Fri, 05 Jul 2019 16:55:24: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:55:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:55:24: #1 tags after filtering in treatment: 1525458 INFO @ Fri, 05 Jul 2019 16:55:24: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 05 Jul 2019 16:55:24: #1 finished! INFO @ Fri, 05 Jul 2019 16:55:24: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:55:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:55:24: #2 number of paired peaks: 10 WARNING @ Fri, 05 Jul 2019 16:55:24: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:55:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:55:28: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 16:55:28: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 16:55:28: #1 total tags in treatment: 1586383 INFO @ Fri, 05 Jul 2019 16:55:28: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:55:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:55:28: #1 tags after filtering in treatment: 1525458 INFO @ Fri, 05 Jul 2019 16:55:28: #1 Redundant rate of treatment: 0.04 INFO @ Fri, 05 Jul 2019 16:55:28: #1 finished! INFO @ Fri, 05 Jul 2019 16:55:28: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:55:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:55:28: #2 number of paired peaks: 10 WARNING @ Fri, 05 Jul 2019 16:55:28: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:55:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX471744/ERX471744.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。