Job ID = 2007834 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-07-05T07:30:06 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T07:30:06 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.73' 2019-07-05T07:30:06 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.73' spots read : 211,136 reads read : 422,272 reads written : 422,272 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:09 211136 reads; of these: 211136 (100.00%) were paired; of these: 29478 (13.96%) aligned concordantly 0 times 166304 (78.77%) aligned concordantly exactly 1 time 15354 (7.27%) aligned concordantly >1 times ---- 29478 pairs aligned concordantly 0 times; of these: 18424 (62.50%) aligned discordantly 1 time ---- 11054 pairs aligned 0 times concordantly or discordantly; of these: 22108 mates make up the pairs; of these: 16218 (73.36%) aligned 0 times 2313 (10.46%) aligned exactly 1 time 3577 (16.18%) aligned >1 times 96.16% overall alignment rate Time searching: 00:00:09 Overall time: 00:00:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 168 / 199590 = 0.0008 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 16:32:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:32:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:32:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:32:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:32:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:32:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:32:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:32:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:32:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:32:34: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:32:34: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:32:34: #1 total tags in treatment: 181513 INFO @ Fri, 05 Jul 2019 16:32:34: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:32:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:32:34: #1 tags after filtering in treatment: 180694 INFO @ Fri, 05 Jul 2019 16:32:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:32:34: #1 finished! INFO @ Fri, 05 Jul 2019 16:32:34: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:32:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:32:34: #2 number of paired peaks: 21 WARNING @ Fri, 05 Jul 2019 16:32:34: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:32:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:32:35: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:32:35: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:32:35: #1 total tags in treatment: 181513 INFO @ Fri, 05 Jul 2019 16:32:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:32:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:32:35: #1 tags after filtering in treatment: 180694 INFO @ Fri, 05 Jul 2019 16:32:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:32:35: #1 finished! INFO @ Fri, 05 Jul 2019 16:32:35: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:32:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:32:36: #2 number of paired peaks: 21 WARNING @ Fri, 05 Jul 2019 16:32:36: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:32:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:32:36: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:32:36: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:32:36: #1 total tags in treatment: 181513 INFO @ Fri, 05 Jul 2019 16:32:36: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:32:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:32:36: #1 tags after filtering in treatment: 180694 INFO @ Fri, 05 Jul 2019 16:32:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:32:36: #1 finished! INFO @ Fri, 05 Jul 2019 16:32:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:32:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:32:36: #2 number of paired peaks: 21 WARNING @ Fri, 05 Jul 2019 16:32:36: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:32:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX462381/ERX462381.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。