Job ID = 14521745 SRX = ERX4439635 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8078203 spots for ERR4501564/ERR4501564.sra Written 8078203 spots for ERR4501564/ERR4501564.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:51 8078203 reads; of these: 8078203 (100.00%) were paired; of these: 1966132 (24.34%) aligned concordantly 0 times 5510325 (68.21%) aligned concordantly exactly 1 time 601746 (7.45%) aligned concordantly >1 times ---- 1966132 pairs aligned concordantly 0 times; of these: 907492 (46.16%) aligned discordantly 1 time ---- 1058640 pairs aligned 0 times concordantly or discordantly; of these: 2117280 mates make up the pairs; of these: 1840566 (86.93%) aligned 0 times 101689 (4.80%) aligned exactly 1 time 175025 (8.27%) aligned >1 times 88.61% overall alignment rate Time searching: 00:07:51 Overall time: 00:07:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1895445 / 6945759 = 0.2729 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:50:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:50:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:50:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:50:51: 1000000 INFO @ Sat, 15 Jan 2022 21:51:00: 2000000 INFO @ Sat, 15 Jan 2022 21:51:09: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:51:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:51:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:51:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:51:18: 4000000 INFO @ Sat, 15 Jan 2022 21:51:22: 1000000 INFO @ Sat, 15 Jan 2022 21:51:29: 5000000 INFO @ Sat, 15 Jan 2022 21:51:32: 2000000 INFO @ Sat, 15 Jan 2022 21:51:38: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:51:41: 3000000 INFO @ Sat, 15 Jan 2022 21:51:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:51:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:51:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:51:49: 7000000 INFO @ Sat, 15 Jan 2022 21:51:51: 4000000 INFO @ Sat, 15 Jan 2022 21:51:56: 1000000 INFO @ Sat, 15 Jan 2022 21:51:59: 8000000 INFO @ Sat, 15 Jan 2022 21:52:01: 5000000 INFO @ Sat, 15 Jan 2022 21:52:09: 9000000 INFO @ Sat, 15 Jan 2022 21:52:09: 2000000 INFO @ Sat, 15 Jan 2022 21:52:11: 6000000 INFO @ Sat, 15 Jan 2022 21:52:18: 10000000 INFO @ Sat, 15 Jan 2022 21:52:21: 7000000 INFO @ Sat, 15 Jan 2022 21:52:23: 3000000 INFO @ Sat, 15 Jan 2022 21:52:23: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:52:23: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:52:23: #1 total tags in treatment: 4350943 INFO @ Sat, 15 Jan 2022 21:52:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:23: #1 tags after filtering in treatment: 2212988 INFO @ Sat, 15 Jan 2022 21:52:23: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 21:52:23: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:24: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 21:52:24: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:52:31: 8000000 INFO @ Sat, 15 Jan 2022 21:52:36: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:52:41: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:52:49: 5000000 INFO @ Sat, 15 Jan 2022 21:52:50: 10000000 INFO @ Sat, 15 Jan 2022 21:52:55: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:52:55: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:52:55: #1 total tags in treatment: 4350943 INFO @ Sat, 15 Jan 2022 21:52:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:55: #1 tags after filtering in treatment: 2212988 INFO @ Sat, 15 Jan 2022 21:52:55: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 21:52:55: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:55: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 21:52:55: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:53:02: 6000000 INFO @ Sat, 15 Jan 2022 21:53:14: 7000000 INFO @ Sat, 15 Jan 2022 21:53:27: 8000000 INFO @ Sat, 15 Jan 2022 21:53:39: 9000000 INFO @ Sat, 15 Jan 2022 21:53:52: 10000000 INFO @ Sat, 15 Jan 2022 21:53:59: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:53:59: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:53:59: #1 total tags in treatment: 4350943 INFO @ Sat, 15 Jan 2022 21:53:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:53:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:53:59: #1 tags after filtering in treatment: 2212988 INFO @ Sat, 15 Jan 2022 21:53:59: #1 Redundant rate of treatment: 0.49 INFO @ Sat, 15 Jan 2022 21:53:59: #1 finished! INFO @ Sat, 15 Jan 2022 21:53:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:53:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:53:59: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 21:53:59: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:53:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439635/ERX4439635.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling