Job ID = 10223874 SRX = ERX4439620 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-10-15T23:32:59 prefetch.2.10.7: 1) Downloading 'ERR4501549'... 2020-10-15T23:32:59 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:33:29 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:33:29 prefetch.2.10.7: 'ERR4501549' is valid 2020-10-15T23:33:29 prefetch.2.10.7: 1) 'ERR4501549' was downloaded successfully 2020-10-15T23:33:57 prefetch.2.10.7: 'ERR4501549' has 5 unresolved dependencies 2020-10-15T23:33:57 prefetch.2.10.7: 2) Downloading 'ncbi-acc:NC_001136.8?vdb-ctx=refseq'... 2020-10-15T23:33:57 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:34:09 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:34:09 prefetch.2.10.7: 2) 'ncbi-acc:NC_001136.8?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:34:09 prefetch.2.10.7: 3) Downloading 'ncbi-acc:NC_001139.7?vdb-ctx=refseq'... 2020-10-15T23:34:09 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:34:17 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:34:17 prefetch.2.10.7: 3) 'ncbi-acc:NC_001139.7?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:34:17 prefetch.2.10.7: 4) Downloading 'ncbi-acc:NC_001144.4?vdb-ctx=refseq'... 2020-10-15T23:34:17 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:34:25 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:34:25 prefetch.2.10.7: 4) 'ncbi-acc:NC_001144.4?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:34:25 prefetch.2.10.7: 5) Downloading 'ncbi-acc:NC_001147.5?vdb-ctx=refseq'... 2020-10-15T23:34:25 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:34:36 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:34:36 prefetch.2.10.7: 5) 'ncbi-acc:NC_001147.5?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:34:36 prefetch.2.10.7: 6) Downloading 'ncbi-acc:NC_001224.1?vdb-ctx=refseq'... 2020-10-15T23:34:36 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:34:46 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:34:46 prefetch.2.10.7: 6) 'ncbi-acc:NC_001224.1?vdb-ctx=refseq' was downloaded successfully Read 3166219 spots for ERR4501549/ERR4501549.sra Written 3166219 spots for ERR4501549/ERR4501549.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:24 3166219 reads; of these: 3166219 (100.00%) were paired; of these: 878070 (27.73%) aligned concordantly 0 times 1974227 (62.35%) aligned concordantly exactly 1 time 313922 (9.91%) aligned concordantly >1 times ---- 878070 pairs aligned concordantly 0 times; of these: 418469 (47.66%) aligned discordantly 1 time ---- 459601 pairs aligned 0 times concordantly or discordantly; of these: 919202 mates make up the pairs; of these: 719434 (78.27%) aligned 0 times 58156 (6.33%) aligned exactly 1 time 141612 (15.41%) aligned >1 times 88.64% overall alignment rate Time searching: 00:02:24 Overall time: 00:02:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 38765 / 2677817 = 0.0145 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:40:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:40:31: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:40:31: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:40:40: 1000000 INFO @ Fri, 16 Oct 2020 08:40:49: 2000000 INFO @ Fri, 16 Oct 2020 08:40:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:41:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:41:01: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:41:01: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:41:08: 4000000 INFO @ Fri, 16 Oct 2020 08:41:10: 1000000 INFO @ Fri, 16 Oct 2020 08:41:18: 5000000 INFO @ Fri, 16 Oct 2020 08:41:18: 2000000 INFO @ Fri, 16 Oct 2020 08:41:23: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:41:23: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:41:23: #1 total tags in treatment: 2256137 INFO @ Fri, 16 Oct 2020 08:41:23: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:41:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:41:23: #1 tags after filtering in treatment: 1996468 INFO @ Fri, 16 Oct 2020 08:41:23: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 16 Oct 2020 08:41:23: #1 finished! INFO @ Fri, 16 Oct 2020 08:41:23: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:41:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:41:23: #2 number of paired peaks: 108 WARNING @ Fri, 16 Oct 2020 08:41:23: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Fri, 16 Oct 2020 08:41:23: start model_add_line... INFO @ Fri, 16 Oct 2020 08:41:23: start X-correlation... INFO @ Fri, 16 Oct 2020 08:41:23: end of X-cor INFO @ Fri, 16 Oct 2020 08:41:23: #2 finished! INFO @ Fri, 16 Oct 2020 08:41:23: #2 predicted fragment length is 149 bps INFO @ Fri, 16 Oct 2020 08:41:23: #2 alternative fragment length(s) may be 2,149 bps INFO @ Fri, 16 Oct 2020 08:41:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.05_model.r WARNING @ Fri, 16 Oct 2020 08:41:23: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:41:23: #2 You may need to consider one of the other alternative d(s): 2,149 WARNING @ Fri, 16 Oct 2020 08:41:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:41:23: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:41:23: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:41:27: 3000000 INFO @ Fri, 16 Oct 2020 08:41:27: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:41:28: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.05_peaks.xls INFO @ Fri, 16 Oct 2020 08:41:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.05_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:41:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.05_summits.bed INFO @ Fri, 16 Oct 2020 08:41:28: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (36 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:41:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:41:31: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:41:31: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:41:35: 4000000 INFO @ Fri, 16 Oct 2020 08:41:40: 1000000 INFO @ Fri, 16 Oct 2020 08:41:43: 5000000 INFO @ Fri, 16 Oct 2020 08:41:48: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:41:48: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:41:48: #1 total tags in treatment: 2256137 INFO @ Fri, 16 Oct 2020 08:41:48: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:41:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:41:48: #1 tags after filtering in treatment: 1996468 INFO @ Fri, 16 Oct 2020 08:41:48: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 16 Oct 2020 08:41:48: #1 finished! INFO @ Fri, 16 Oct 2020 08:41:48: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:41:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:41:48: #2 number of paired peaks: 108 WARNING @ Fri, 16 Oct 2020 08:41:48: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Fri, 16 Oct 2020 08:41:48: start model_add_line... INFO @ Fri, 16 Oct 2020 08:41:48: start X-correlation... INFO @ Fri, 16 Oct 2020 08:41:48: end of X-cor INFO @ Fri, 16 Oct 2020 08:41:48: #2 finished! INFO @ Fri, 16 Oct 2020 08:41:48: #2 predicted fragment length is 149 bps INFO @ Fri, 16 Oct 2020 08:41:48: #2 alternative fragment length(s) may be 2,149 bps INFO @ Fri, 16 Oct 2020 08:41:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.10_model.r WARNING @ Fri, 16 Oct 2020 08:41:48: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:41:48: #2 You may need to consider one of the other alternative d(s): 2,149 WARNING @ Fri, 16 Oct 2020 08:41:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:41:48: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:41:48: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:41:48: 2000000 INFO @ Fri, 16 Oct 2020 08:41:52: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:41:54: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.10_peaks.xls INFO @ Fri, 16 Oct 2020 08:41:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.10_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:41:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.10_summits.bed INFO @ Fri, 16 Oct 2020 08:41:54: Done! pass1 - making usageList (4 chroms): 0 millis pass2 - checking and writing primary data (15 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:41:56: 3000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:42:04: 4000000 INFO @ Fri, 16 Oct 2020 08:42:12: 5000000 INFO @ Fri, 16 Oct 2020 08:42:16: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:42:16: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:42:16: #1 total tags in treatment: 2256137 INFO @ Fri, 16 Oct 2020 08:42:16: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:42:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:42:16: #1 tags after filtering in treatment: 1996468 INFO @ Fri, 16 Oct 2020 08:42:16: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 16 Oct 2020 08:42:16: #1 finished! INFO @ Fri, 16 Oct 2020 08:42:16: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:42:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:42:17: #2 number of paired peaks: 108 WARNING @ Fri, 16 Oct 2020 08:42:17: Fewer paired peaks (108) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 108 pairs to build model! INFO @ Fri, 16 Oct 2020 08:42:17: start model_add_line... INFO @ Fri, 16 Oct 2020 08:42:17: start X-correlation... INFO @ Fri, 16 Oct 2020 08:42:17: end of X-cor INFO @ Fri, 16 Oct 2020 08:42:17: #2 finished! INFO @ Fri, 16 Oct 2020 08:42:17: #2 predicted fragment length is 149 bps INFO @ Fri, 16 Oct 2020 08:42:17: #2 alternative fragment length(s) may be 2,149 bps INFO @ Fri, 16 Oct 2020 08:42:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.20_model.r WARNING @ Fri, 16 Oct 2020 08:42:17: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:42:17: #2 You may need to consider one of the other alternative d(s): 2,149 WARNING @ Fri, 16 Oct 2020 08:42:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:42:17: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:42:17: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:42:21: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:42:22: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.20_peaks.xls INFO @ Fri, 16 Oct 2020 08:42:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.20_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:42:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439620/ERX4439620.20_summits.bed INFO @ Fri, 16 Oct 2020 08:42:22: Done! pass1 - making usageList (2 chroms): 1 millis pass2 - checking and writing primary data (2 records, 4 fields): 1 millis CompletedMACS2peakCalling