Job ID = 14521657 SRX = ERX4439608 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3886184 spots for ERR4501537/ERR4501537.sra Written 3886184 spots for ERR4501537/ERR4501537.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:54 3886184 reads; of these: 3886184 (100.00%) were paired; of these: 1197487 (30.81%) aligned concordantly 0 times 2274409 (58.53%) aligned concordantly exactly 1 time 414288 (10.66%) aligned concordantly >1 times ---- 1197487 pairs aligned concordantly 0 times; of these: 450608 (37.63%) aligned discordantly 1 time ---- 746879 pairs aligned 0 times concordantly or discordantly; of these: 1493758 mates make up the pairs; of these: 1254783 (84.00%) aligned 0 times 63605 (4.26%) aligned exactly 1 time 175370 (11.74%) aligned >1 times 83.86% overall alignment rate Time searching: 00:02:54 Overall time: 00:02:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 51028 / 3107736 = 0.0164 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:28:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:28:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:28:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:28:19: 1000000 INFO @ Sat, 15 Jan 2022 21:28:26: 2000000 INFO @ Sat, 15 Jan 2022 21:28:34: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:28:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:28:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:28:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:28:41: 4000000 INFO @ Sat, 15 Jan 2022 21:28:50: 5000000 INFO @ Sat, 15 Jan 2022 21:28:50: 1000000 INFO @ Sat, 15 Jan 2022 21:28:58: 6000000 INFO @ Sat, 15 Jan 2022 21:28:58: 2000000 INFO @ Sat, 15 Jan 2022 21:29:01: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:29:01: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:29:01: #1 total tags in treatment: 2645579 INFO @ Sat, 15 Jan 2022 21:29:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:29:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:29:01: #1 tags after filtering in treatment: 2272906 INFO @ Sat, 15 Jan 2022 21:29:01: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:29:01: #1 finished! INFO @ Sat, 15 Jan 2022 21:29:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:29:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:29:01: #2 number of paired peaks: 118 WARNING @ Sat, 15 Jan 2022 21:29:01: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Sat, 15 Jan 2022 21:29:01: start model_add_line... INFO @ Sat, 15 Jan 2022 21:29:01: start X-correlation... INFO @ Sat, 15 Jan 2022 21:29:01: end of X-cor INFO @ Sat, 15 Jan 2022 21:29:01: #2 finished! INFO @ Sat, 15 Jan 2022 21:29:01: #2 predicted fragment length is 139 bps INFO @ Sat, 15 Jan 2022 21:29:01: #2 alternative fragment length(s) may be 1,117,139,152,179,185,592,598 bps INFO @ Sat, 15 Jan 2022 21:29:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.05_model.r WARNING @ Sat, 15 Jan 2022 21:29:01: #2 Since the d (139) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Jan 2022 21:29:01: #2 You may need to consider one of the other alternative d(s): 1,117,139,152,179,185,592,598 WARNING @ Sat, 15 Jan 2022 21:29:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 15 Jan 2022 21:29:01: #3 Call peaks... INFO @ Sat, 15 Jan 2022 21:29:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 21:29:06: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 21:29:06: 3000000 INFO @ Sat, 15 Jan 2022 21:29:07: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.05_peaks.xls INFO @ Sat, 15 Jan 2022 21:29:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.05_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 21:29:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.05_summits.bed INFO @ Sat, 15 Jan 2022 21:29:07: Done! pass1 - making usageList (17 chroms): 0 millis pass2 - checking and writing primary data (84 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:14: 4000000 INFO @ Sat, 15 Jan 2022 21:29:19: 1000000 INFO @ Sat, 15 Jan 2022 21:29:23: 5000000 INFO @ Sat, 15 Jan 2022 21:29:28: 2000000 INFO @ Sat, 15 Jan 2022 21:29:31: 6000000 INFO @ Sat, 15 Jan 2022 21:29:34: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:29:34: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:29:34: #1 total tags in treatment: 2645579 INFO @ Sat, 15 Jan 2022 21:29:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:29:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:29:35: #1 tags after filtering in treatment: 2272906 INFO @ Sat, 15 Jan 2022 21:29:35: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:29:35: #1 finished! INFO @ Sat, 15 Jan 2022 21:29:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:29:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:29:35: #2 number of paired peaks: 118 WARNING @ Sat, 15 Jan 2022 21:29:35: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Sat, 15 Jan 2022 21:29:35: start model_add_line... INFO @ Sat, 15 Jan 2022 21:29:35: start X-correlation... INFO @ Sat, 15 Jan 2022 21:29:35: end of X-cor INFO @ Sat, 15 Jan 2022 21:29:35: #2 finished! INFO @ Sat, 15 Jan 2022 21:29:35: #2 predicted fragment length is 139 bps INFO @ Sat, 15 Jan 2022 21:29:35: #2 alternative fragment length(s) may be 1,117,139,152,179,185,592,598 bps INFO @ Sat, 15 Jan 2022 21:29:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.10_model.r WARNING @ Sat, 15 Jan 2022 21:29:35: #2 Since the d (139) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Jan 2022 21:29:35: #2 You may need to consider one of the other alternative d(s): 1,117,139,152,179,185,592,598 WARNING @ Sat, 15 Jan 2022 21:29:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 15 Jan 2022 21:29:35: #3 Call peaks... INFO @ Sat, 15 Jan 2022 21:29:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 21:29:36: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:29:39: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 21:29:41: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.10_peaks.xls INFO @ Sat, 15 Jan 2022 21:29:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.10_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 21:29:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.10_summits.bed INFO @ Sat, 15 Jan 2022 21:29:41: Done! pass1 - making usageList (17 chroms): 0 millis pass2 - checking and writing primary data (38 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:29:44: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:29:52: 5000000 INFO @ Sat, 15 Jan 2022 21:29:59: 6000000 INFO @ Sat, 15 Jan 2022 21:30:02: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:30:02: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:30:02: #1 total tags in treatment: 2645579 INFO @ Sat, 15 Jan 2022 21:30:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:30:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:30:02: #1 tags after filtering in treatment: 2272906 INFO @ Sat, 15 Jan 2022 21:30:02: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:30:02: #1 finished! INFO @ Sat, 15 Jan 2022 21:30:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:30:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:30:03: #2 number of paired peaks: 118 WARNING @ Sat, 15 Jan 2022 21:30:03: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Sat, 15 Jan 2022 21:30:03: start model_add_line... INFO @ Sat, 15 Jan 2022 21:30:03: start X-correlation... INFO @ Sat, 15 Jan 2022 21:30:03: end of X-cor INFO @ Sat, 15 Jan 2022 21:30:03: #2 finished! INFO @ Sat, 15 Jan 2022 21:30:03: #2 predicted fragment length is 139 bps INFO @ Sat, 15 Jan 2022 21:30:03: #2 alternative fragment length(s) may be 1,117,139,152,179,185,592,598 bps INFO @ Sat, 15 Jan 2022 21:30:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.20_model.r WARNING @ Sat, 15 Jan 2022 21:30:03: #2 Since the d (139) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Jan 2022 21:30:03: #2 You may need to consider one of the other alternative d(s): 1,117,139,152,179,185,592,598 WARNING @ Sat, 15 Jan 2022 21:30:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 15 Jan 2022 21:30:03: #3 Call peaks... INFO @ Sat, 15 Jan 2022 21:30:03: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 21:30:07: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 21:30:08: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.20_peaks.xls INFO @ Sat, 15 Jan 2022 21:30:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.20_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 21:30:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439608/ERX4439608.20_summits.bed INFO @ Sat, 15 Jan 2022 21:30:08: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (15 records, 4 fields): 1 millis CompletedMACS2peakCalling