Job ID = 14521655 SRX = ERX4439607 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4421401 spots for ERR4501536/ERR4501536.sra Written 4421401 spots for ERR4501536/ERR4501536.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:32 4421401 reads; of these: 4421401 (100.00%) were paired; of these: 1409323 (31.88%) aligned concordantly 0 times 2545017 (57.56%) aligned concordantly exactly 1 time 467061 (10.56%) aligned concordantly >1 times ---- 1409323 pairs aligned concordantly 0 times; of these: 569240 (40.39%) aligned discordantly 1 time ---- 840083 pairs aligned 0 times concordantly or discordantly; of these: 1680166 mates make up the pairs; of these: 1381581 (82.23%) aligned 0 times 76759 (4.57%) aligned exactly 1 time 221826 (13.20%) aligned >1 times 84.38% overall alignment rate Time searching: 00:03:32 Overall time: 00:03:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 68865 / 3542327 = 0.0194 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:09: 1000000 INFO @ Sat, 15 Jan 2022 21:29:15: 2000000 INFO @ Sat, 15 Jan 2022 21:29:22: 3000000 INFO @ Sat, 15 Jan 2022 21:29:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:37: 5000000 INFO @ Sat, 15 Jan 2022 21:29:42: 1000000 INFO @ Sat, 15 Jan 2022 21:29:46: 6000000 INFO @ Sat, 15 Jan 2022 21:29:51: 2000000 INFO @ Sat, 15 Jan 2022 21:29:55: 7000000 INFO @ Sat, 15 Jan 2022 21:29:58: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:29:58: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:29:58: #1 total tags in treatment: 2954020 INFO @ Sat, 15 Jan 2022 21:29:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:29:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:29:58: #1 tags after filtering in treatment: 2528885 INFO @ Sat, 15 Jan 2022 21:29:58: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:29:58: #1 finished! INFO @ Sat, 15 Jan 2022 21:29:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:29:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:29:58: #2 number of paired peaks: 77 WARNING @ Sat, 15 Jan 2022 21:29:58: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:29:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:30:01: 3000000 INFO @ Sat, 15 Jan 2022 21:30:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:30:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:30:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:30:12: 1000000 INFO @ Sat, 15 Jan 2022 21:30:12: 4000000 INFO @ Sat, 15 Jan 2022 21:30:21: 2000000 INFO @ Sat, 15 Jan 2022 21:30:22: 5000000 INFO @ Sat, 15 Jan 2022 21:30:31: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:30:33: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:30:41: 4000000 INFO @ Sat, 15 Jan 2022 21:30:44: 7000000 INFO @ Sat, 15 Jan 2022 21:30:47: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:30:47: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:30:47: #1 total tags in treatment: 2954020 INFO @ Sat, 15 Jan 2022 21:30:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:30:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:30:47: #1 tags after filtering in treatment: 2528885 INFO @ Sat, 15 Jan 2022 21:30:47: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:30:47: #1 finished! INFO @ Sat, 15 Jan 2022 21:30:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:30:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:30:47: #2 number of paired peaks: 77 WARNING @ Sat, 15 Jan 2022 21:30:47: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:30:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:30:51: 5000000 INFO @ Sat, 15 Jan 2022 21:31:00: 6000000 INFO @ Sat, 15 Jan 2022 21:31:09: 7000000 INFO @ Sat, 15 Jan 2022 21:31:12: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:31:12: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:31:12: #1 total tags in treatment: 2954020 INFO @ Sat, 15 Jan 2022 21:31:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:31:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:31:12: #1 tags after filtering in treatment: 2528885 INFO @ Sat, 15 Jan 2022 21:31:12: #1 Redundant rate of treatment: 0.14 INFO @ Sat, 15 Jan 2022 21:31:12: #1 finished! INFO @ Sat, 15 Jan 2022 21:31:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:31:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:31:12: #2 number of paired peaks: 77 WARNING @ Sat, 15 Jan 2022 21:31:12: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:31:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439607/ERX4439607.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling