Job ID = 10223820 SRX = ERX4439580 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-10-15T23:15:07 prefetch.2.10.7: 1) Downloading 'ERR4501509'... 2020-10-15T23:15:07 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:15:49 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:15:50 prefetch.2.10.7: 'ERR4501509' is valid 2020-10-15T23:15:50 prefetch.2.10.7: 1) 'ERR4501509' was downloaded successfully 2020-10-15T23:16:18 prefetch.2.10.7: 'ERR4501509' has 5 unresolved dependencies 2020-10-15T23:16:18 prefetch.2.10.7: 2) Downloading 'ncbi-acc:NC_001136.8?vdb-ctx=refseq'... 2020-10-15T23:16:18 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:16:26 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:16:26 prefetch.2.10.7: 2) 'ncbi-acc:NC_001136.8?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:16:26 prefetch.2.10.7: 3) Downloading 'ncbi-acc:NC_001139.7?vdb-ctx=refseq'... 2020-10-15T23:16:26 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:16:39 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:16:39 prefetch.2.10.7: 3) 'ncbi-acc:NC_001139.7?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:16:39 prefetch.2.10.7: 4) Downloading 'ncbi-acc:NC_001144.4?vdb-ctx=refseq'... 2020-10-15T23:16:39 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:16:52 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:16:52 prefetch.2.10.7: 4) 'ncbi-acc:NC_001144.4?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:16:52 prefetch.2.10.7: 5) Downloading 'ncbi-acc:NC_001147.5?vdb-ctx=refseq'... 2020-10-15T23:16:52 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:16:59 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:16:59 prefetch.2.10.7: 5) 'ncbi-acc:NC_001147.5?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:16:59 prefetch.2.10.7: 6) Downloading 'ncbi-acc:NC_001224.1?vdb-ctx=refseq'... 2020-10-15T23:16:59 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:17:09 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:17:09 prefetch.2.10.7: 6) 'ncbi-acc:NC_001224.1?vdb-ctx=refseq' was downloaded successfully Read 3474580 spots for ERR4501509/ERR4501509.sra Written 3474580 spots for ERR4501509/ERR4501509.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:31 3474580 reads; of these: 3474580 (100.00%) were paired; of these: 733137 (21.10%) aligned concordantly 0 times 2421965 (69.71%) aligned concordantly exactly 1 time 319478 (9.19%) aligned concordantly >1 times ---- 733137 pairs aligned concordantly 0 times; of these: 382206 (52.13%) aligned discordantly 1 time ---- 350931 pairs aligned 0 times concordantly or discordantly; of these: 701862 mates make up the pairs; of these: 544273 (77.55%) aligned 0 times 52144 (7.43%) aligned exactly 1 time 105445 (15.02%) aligned >1 times 92.17% overall alignment rate Time searching: 00:02:31 Overall time: 00:02:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 98034 / 3096365 = 0.0317 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:23:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:23:26: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:23:26: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:23:35: 1000000 INFO @ Fri, 16 Oct 2020 08:23:43: 2000000 INFO @ Fri, 16 Oct 2020 08:23:52: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:23:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:23:56: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:23:56: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:24:01: 4000000 INFO @ Fri, 16 Oct 2020 08:24:05: 1000000 INFO @ Fri, 16 Oct 2020 08:24:10: 5000000 INFO @ Fri, 16 Oct 2020 08:24:15: 2000000 INFO @ Fri, 16 Oct 2020 08:24:19: 6000000 INFO @ Fri, 16 Oct 2020 08:24:21: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:24:21: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:24:21: #1 total tags in treatment: 2666066 INFO @ Fri, 16 Oct 2020 08:24:21: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:24:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:24:21: #1 tags after filtering in treatment: 1940757 INFO @ Fri, 16 Oct 2020 08:24:21: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 16 Oct 2020 08:24:21: #1 finished! INFO @ Fri, 16 Oct 2020 08:24:21: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:24:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:24:21: #2 number of paired peaks: 243 WARNING @ Fri, 16 Oct 2020 08:24:21: Fewer paired peaks (243) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 243 pairs to build model! INFO @ Fri, 16 Oct 2020 08:24:21: start model_add_line... INFO @ Fri, 16 Oct 2020 08:24:21: start X-correlation... INFO @ Fri, 16 Oct 2020 08:24:21: end of X-cor INFO @ Fri, 16 Oct 2020 08:24:21: #2 finished! INFO @ Fri, 16 Oct 2020 08:24:21: #2 predicted fragment length is 178 bps INFO @ Fri, 16 Oct 2020 08:24:21: #2 alternative fragment length(s) may be 178 bps INFO @ Fri, 16 Oct 2020 08:24:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.05_model.r WARNING @ Fri, 16 Oct 2020 08:24:21: #2 Since the d (178) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:24:21: #2 You may need to consider one of the other alternative d(s): 178 WARNING @ Fri, 16 Oct 2020 08:24:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:24:21: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:24:21: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:24:24: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:24:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:24:26: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:24:26: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:24:27: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:24:28: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.05_peaks.xls INFO @ Fri, 16 Oct 2020 08:24:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.05_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:24:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.05_summits.bed INFO @ Fri, 16 Oct 2020 08:24:28: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (1564 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:24:33: 4000000 INFO @ Fri, 16 Oct 2020 08:24:35: 1000000 INFO @ Fri, 16 Oct 2020 08:24:42: 5000000 INFO @ Fri, 16 Oct 2020 08:24:45: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:24:52: 6000000 INFO @ Fri, 16 Oct 2020 08:24:54: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:24:54: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:24:54: #1 total tags in treatment: 2666066 INFO @ Fri, 16 Oct 2020 08:24:54: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:24:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:24:54: #1 tags after filtering in treatment: 1940757 INFO @ Fri, 16 Oct 2020 08:24:54: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 16 Oct 2020 08:24:54: #1 finished! INFO @ Fri, 16 Oct 2020 08:24:54: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:24:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:24:54: #2 number of paired peaks: 243 WARNING @ Fri, 16 Oct 2020 08:24:54: Fewer paired peaks (243) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 243 pairs to build model! INFO @ Fri, 16 Oct 2020 08:24:54: start model_add_line... INFO @ Fri, 16 Oct 2020 08:24:54: start X-correlation... INFO @ Fri, 16 Oct 2020 08:24:54: end of X-cor INFO @ Fri, 16 Oct 2020 08:24:54: #2 finished! INFO @ Fri, 16 Oct 2020 08:24:54: #2 predicted fragment length is 178 bps INFO @ Fri, 16 Oct 2020 08:24:54: #2 alternative fragment length(s) may be 178 bps INFO @ Fri, 16 Oct 2020 08:24:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.10_model.r WARNING @ Fri, 16 Oct 2020 08:24:54: #2 Since the d (178) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:24:54: #2 You may need to consider one of the other alternative d(s): 178 WARNING @ Fri, 16 Oct 2020 08:24:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:24:54: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:24:54: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:24:55: 3000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:24:59: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:25:01: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.10_peaks.xls INFO @ Fri, 16 Oct 2020 08:25:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.10_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:25:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.10_summits.bed INFO @ Fri, 16 Oct 2020 08:25:01: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (1220 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:25:04: 4000000 INFO @ Fri, 16 Oct 2020 08:25:13: 5000000 INFO @ Fri, 16 Oct 2020 08:25:22: 6000000 INFO @ Fri, 16 Oct 2020 08:25:24: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:25:24: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:25:24: #1 total tags in treatment: 2666066 INFO @ Fri, 16 Oct 2020 08:25:24: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:25:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:25:24: #1 tags after filtering in treatment: 1940757 INFO @ Fri, 16 Oct 2020 08:25:24: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 16 Oct 2020 08:25:24: #1 finished! INFO @ Fri, 16 Oct 2020 08:25:24: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:25:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:25:24: #2 number of paired peaks: 243 WARNING @ Fri, 16 Oct 2020 08:25:24: Fewer paired peaks (243) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 243 pairs to build model! INFO @ Fri, 16 Oct 2020 08:25:24: start model_add_line... INFO @ Fri, 16 Oct 2020 08:25:24: start X-correlation... INFO @ Fri, 16 Oct 2020 08:25:24: end of X-cor INFO @ Fri, 16 Oct 2020 08:25:24: #2 finished! INFO @ Fri, 16 Oct 2020 08:25:24: #2 predicted fragment length is 178 bps INFO @ Fri, 16 Oct 2020 08:25:24: #2 alternative fragment length(s) may be 178 bps INFO @ Fri, 16 Oct 2020 08:25:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.20_model.r WARNING @ Fri, 16 Oct 2020 08:25:24: #2 Since the d (178) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:25:24: #2 You may need to consider one of the other alternative d(s): 178 WARNING @ Fri, 16 Oct 2020 08:25:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:25:24: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:25:24: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:25:30: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:25:32: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.20_peaks.xls INFO @ Fri, 16 Oct 2020 08:25:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.20_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:25:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439580/ERX4439580.20_summits.bed INFO @ Fri, 16 Oct 2020 08:25:32: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (886 records, 4 fields): 2 millis CompletedMACS2peakCalling