Job ID = 10223802 SRX = ERX4439566 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-10-15T23:07:46 prefetch.2.10.7: 1) Downloading 'ERR4501495'... 2020-10-15T23:07:46 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:08:21 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:08:21 prefetch.2.10.7: 'ERR4501495' is valid 2020-10-15T23:08:21 prefetch.2.10.7: 1) 'ERR4501495' was downloaded successfully 2020-10-15T23:08:49 prefetch.2.10.7: 'ERR4501495' has 5 unresolved dependencies 2020-10-15T23:08:49 prefetch.2.10.7: 2) Downloading 'ncbi-acc:NC_001136.8?vdb-ctx=refseq'... 2020-10-15T23:08:49 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:08:58 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:08:58 prefetch.2.10.7: 2) 'ncbi-acc:NC_001136.8?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:08:58 prefetch.2.10.7: 3) Downloading 'ncbi-acc:NC_001139.7?vdb-ctx=refseq'... 2020-10-15T23:08:58 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:09:10 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:09:10 prefetch.2.10.7: 3) 'ncbi-acc:NC_001139.7?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:09:10 prefetch.2.10.7: 4) Downloading 'ncbi-acc:NC_001144.4?vdb-ctx=refseq'... 2020-10-15T23:09:10 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:09:18 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:09:18 prefetch.2.10.7: 4) 'ncbi-acc:NC_001144.4?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:09:18 prefetch.2.10.7: 5) Downloading 'ncbi-acc:NC_001147.5?vdb-ctx=refseq'... 2020-10-15T23:09:18 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:09:30 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:09:30 prefetch.2.10.7: 5) 'ncbi-acc:NC_001147.5?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:09:30 prefetch.2.10.7: 6) Downloading 'ncbi-acc:NC_001224.1?vdb-ctx=refseq'... 2020-10-15T23:09:30 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:09:39 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:09:39 prefetch.2.10.7: 6) 'ncbi-acc:NC_001224.1?vdb-ctx=refseq' was downloaded successfully Read 3316887 spots for ERR4501495/ERR4501495.sra Written 3316887 spots for ERR4501495/ERR4501495.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:25 3316887 reads; of these: 3316887 (100.00%) were paired; of these: 924940 (27.89%) aligned concordantly 0 times 2016876 (60.81%) aligned concordantly exactly 1 time 375071 (11.31%) aligned concordantly >1 times ---- 924940 pairs aligned concordantly 0 times; of these: 355501 (38.44%) aligned discordantly 1 time ---- 569439 pairs aligned 0 times concordantly or discordantly; of these: 1138878 mates make up the pairs; of these: 934809 (82.08%) aligned 0 times 50792 (4.46%) aligned exactly 1 time 153277 (13.46%) aligned >1 times 85.91% overall alignment rate Time searching: 00:02:25 Overall time: 00:02:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 49845 / 2720878 = 0.0183 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:15:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:15:34: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:15:34: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:15:40: 1000000 INFO @ Fri, 16 Oct 2020 08:15:47: 2000000 INFO @ Fri, 16 Oct 2020 08:15:53: 3000000 INFO @ Fri, 16 Oct 2020 08:16:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:16:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:16:04: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:16:04: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:16:07: 5000000 INFO @ Fri, 16 Oct 2020 08:16:11: 1000000 INFO @ Fri, 16 Oct 2020 08:16:11: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:16:11: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:16:11: #1 total tags in treatment: 2348751 INFO @ Fri, 16 Oct 2020 08:16:11: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:16:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:16:11: #1 tags after filtering in treatment: 2020541 INFO @ Fri, 16 Oct 2020 08:16:11: #1 Redundant rate of treatment: 0.14 INFO @ Fri, 16 Oct 2020 08:16:11: #1 finished! INFO @ Fri, 16 Oct 2020 08:16:11: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:16:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:16:11: #2 number of paired peaks: 163 WARNING @ Fri, 16 Oct 2020 08:16:11: Fewer paired peaks (163) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 163 pairs to build model! INFO @ Fri, 16 Oct 2020 08:16:11: start model_add_line... INFO @ Fri, 16 Oct 2020 08:16:11: start X-correlation... INFO @ Fri, 16 Oct 2020 08:16:11: end of X-cor INFO @ Fri, 16 Oct 2020 08:16:11: #2 finished! INFO @ Fri, 16 Oct 2020 08:16:11: #2 predicted fragment length is 140 bps INFO @ Fri, 16 Oct 2020 08:16:11: #2 alternative fragment length(s) may be 0,140,518,571 bps INFO @ Fri, 16 Oct 2020 08:16:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.05_model.r WARNING @ Fri, 16 Oct 2020 08:16:11: #2 Since the d (140) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:16:11: #2 You may need to consider one of the other alternative d(s): 0,140,518,571 WARNING @ Fri, 16 Oct 2020 08:16:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:16:11: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:16:11: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:16:15: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:16:17: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.05_peaks.xls INFO @ Fri, 16 Oct 2020 08:16:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.05_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:16:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.05_summits.bed INFO @ Fri, 16 Oct 2020 08:16:17: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (42 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:16:18: 2000000 INFO @ Fri, 16 Oct 2020 08:16:25: 3000000 INFO @ Fri, 16 Oct 2020 08:16:32: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:16:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:16:34: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:16:34: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:16:39: 5000000 INFO @ Fri, 16 Oct 2020 08:16:40: 1000000 INFO @ Fri, 16 Oct 2020 08:16:43: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:16:43: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:16:43: #1 total tags in treatment: 2348751 INFO @ Fri, 16 Oct 2020 08:16:43: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:16:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:16:43: #1 tags after filtering in treatment: 2020541 INFO @ Fri, 16 Oct 2020 08:16:43: #1 Redundant rate of treatment: 0.14 INFO @ Fri, 16 Oct 2020 08:16:43: #1 finished! INFO @ Fri, 16 Oct 2020 08:16:43: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:16:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:16:43: #2 number of paired peaks: 163 WARNING @ Fri, 16 Oct 2020 08:16:43: Fewer paired peaks (163) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 163 pairs to build model! INFO @ Fri, 16 Oct 2020 08:16:43: start model_add_line... INFO @ Fri, 16 Oct 2020 08:16:43: start X-correlation... INFO @ Fri, 16 Oct 2020 08:16:43: end of X-cor INFO @ Fri, 16 Oct 2020 08:16:43: #2 finished! INFO @ Fri, 16 Oct 2020 08:16:43: #2 predicted fragment length is 140 bps INFO @ Fri, 16 Oct 2020 08:16:43: #2 alternative fragment length(s) may be 0,140,518,571 bps INFO @ Fri, 16 Oct 2020 08:16:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.10_model.r WARNING @ Fri, 16 Oct 2020 08:16:43: #2 Since the d (140) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:16:43: #2 You may need to consider one of the other alternative d(s): 0,140,518,571 WARNING @ Fri, 16 Oct 2020 08:16:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:16:43: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:16:43: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:16:46: 2000000 INFO @ Fri, 16 Oct 2020 08:16:47: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:16:49: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.10_peaks.xls INFO @ Fri, 16 Oct 2020 08:16:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.10_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:16:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.10_summits.bed INFO @ Fri, 16 Oct 2020 08:16:49: Done! pass1 - making usageList (5 chroms): 0 millis pass2 - checking and writing primary data (16 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:16:53: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:16:59: 4000000 INFO @ Fri, 16 Oct 2020 08:17:04: 5000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:17:08: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:17:08: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:17:08: #1 total tags in treatment: 2348751 INFO @ Fri, 16 Oct 2020 08:17:08: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:17:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:17:08: #1 tags after filtering in treatment: 2020541 INFO @ Fri, 16 Oct 2020 08:17:08: #1 Redundant rate of treatment: 0.14 INFO @ Fri, 16 Oct 2020 08:17:08: #1 finished! INFO @ Fri, 16 Oct 2020 08:17:08: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:17:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:17:08: #2 number of paired peaks: 163 WARNING @ Fri, 16 Oct 2020 08:17:08: Fewer paired peaks (163) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 163 pairs to build model! INFO @ Fri, 16 Oct 2020 08:17:08: start model_add_line... INFO @ Fri, 16 Oct 2020 08:17:08: start X-correlation... INFO @ Fri, 16 Oct 2020 08:17:08: end of X-cor INFO @ Fri, 16 Oct 2020 08:17:08: #2 finished! INFO @ Fri, 16 Oct 2020 08:17:08: #2 predicted fragment length is 140 bps INFO @ Fri, 16 Oct 2020 08:17:08: #2 alternative fragment length(s) may be 0,140,518,571 bps INFO @ Fri, 16 Oct 2020 08:17:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.20_model.r WARNING @ Fri, 16 Oct 2020 08:17:08: #2 Since the d (140) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:17:08: #2 You may need to consider one of the other alternative d(s): 0,140,518,571 WARNING @ Fri, 16 Oct 2020 08:17:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:17:08: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:17:08: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:17:12: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:17:14: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.20_peaks.xls INFO @ Fri, 16 Oct 2020 08:17:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.20_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:17:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439566/ERX4439566.20_summits.bed INFO @ Fri, 16 Oct 2020 08:17:14: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (1 records, 4 fields): 1 millis CompletedMACS2peakCalling