Job ID = 14521820 SRX = ERX4439543 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3197860 spots for ERR4501472/ERR4501472.sra Written 3197860 spots for ERR4501472/ERR4501472.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:37 3197860 reads; of these: 3197860 (100.00%) were paired; of these: 841027 (26.30%) aligned concordantly 0 times 2016274 (63.05%) aligned concordantly exactly 1 time 340559 (10.65%) aligned concordantly >1 times ---- 841027 pairs aligned concordantly 0 times; of these: 391955 (46.60%) aligned discordantly 1 time ---- 449072 pairs aligned 0 times concordantly or discordantly; of these: 898144 mates make up the pairs; of these: 696640 (77.56%) aligned 0 times 53774 (5.99%) aligned exactly 1 time 147730 (16.45%) aligned >1 times 89.11% overall alignment rate Time searching: 00:02:37 Overall time: 00:02:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 43007 / 2720898 = 0.0158 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:47:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:47:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:47:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:47:18: 1000000 INFO @ Sat, 15 Jan 2022 21:47:24: 2000000 INFO @ Sat, 15 Jan 2022 21:47:31: 3000000 INFO @ Sat, 15 Jan 2022 21:47:37: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:47:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:47:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:47:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:47:45: 5000000 INFO @ Sat, 15 Jan 2022 21:47:49: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:47:49: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:47:49: #1 total tags in treatment: 2320102 INFO @ Sat, 15 Jan 2022 21:47:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:47:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:47:49: #1 tags after filtering in treatment: 2035913 INFO @ Sat, 15 Jan 2022 21:47:49: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 15 Jan 2022 21:47:49: #1 finished! INFO @ Sat, 15 Jan 2022 21:47:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:47:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:47:49: 1000000 INFO @ Sat, 15 Jan 2022 21:47:49: #2 number of paired peaks: 77 WARNING @ Sat, 15 Jan 2022 21:47:49: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:47:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:47:56: 2000000 INFO @ Sat, 15 Jan 2022 21:48:03: 3000000 INFO @ Sat, 15 Jan 2022 21:48:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:18: 5000000 INFO @ Sat, 15 Jan 2022 21:48:21: 1000000 INFO @ Sat, 15 Jan 2022 21:48:22: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:48:22: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:48:22: #1 total tags in treatment: 2320102 INFO @ Sat, 15 Jan 2022 21:48:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:22: #1 tags after filtering in treatment: 2035913 INFO @ Sat, 15 Jan 2022 21:48:22: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 15 Jan 2022 21:48:22: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:22: #2 number of paired peaks: 77 WARNING @ Sat, 15 Jan 2022 21:48:22: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:48:29: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:48:38: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:48:46: 4000000 INFO @ Sat, 15 Jan 2022 21:48:54: 5000000 INFO @ Sat, 15 Jan 2022 21:48:59: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:48:59: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:48:59: #1 total tags in treatment: 2320102 INFO @ Sat, 15 Jan 2022 21:48:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:59: #1 tags after filtering in treatment: 2035913 INFO @ Sat, 15 Jan 2022 21:48:59: #1 Redundant rate of treatment: 0.12 INFO @ Sat, 15 Jan 2022 21:48:59: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:00: #2 number of paired peaks: 77 WARNING @ Sat, 15 Jan 2022 21:49:00: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439543/ERX4439543.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling