Job ID = 14521819 SRX = ERX4439542 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3327107 spots for ERR4501471/ERR4501471.sra Written 3327107 spots for ERR4501471/ERR4501471.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:34 3327107 reads; of these: 3327107 (100.00%) were paired; of these: 951600 (28.60%) aligned concordantly 0 times 2053295 (61.71%) aligned concordantly exactly 1 time 322212 (9.68%) aligned concordantly >1 times ---- 951600 pairs aligned concordantly 0 times; of these: 420104 (44.15%) aligned discordantly 1 time ---- 531496 pairs aligned 0 times concordantly or discordantly; of these: 1062992 mates make up the pairs; of these: 866126 (81.48%) aligned 0 times 54497 (5.13%) aligned exactly 1 time 142369 (13.39%) aligned >1 times 86.98% overall alignment rate Time searching: 00:02:34 Overall time: 00:02:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 43047 / 2766053 = 0.0156 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:47:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:47:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:47:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:47:40: 1000000 INFO @ Sat, 15 Jan 2022 21:47:47: 2000000 INFO @ Sat, 15 Jan 2022 21:47:54: 3000000 INFO @ Sat, 15 Jan 2022 21:48:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:08: 5000000 INFO @ Sat, 15 Jan 2022 21:48:09: 1000000 INFO @ Sat, 15 Jan 2022 21:48:14: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:48:14: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:48:14: #1 total tags in treatment: 2339783 INFO @ Sat, 15 Jan 2022 21:48:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:14: #1 tags after filtering in treatment: 2071476 INFO @ Sat, 15 Jan 2022 21:48:14: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 15 Jan 2022 21:48:14: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:14: #2 number of paired peaks: 98 WARNING @ Sat, 15 Jan 2022 21:48:14: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:48:15: 2000000 INFO @ Sat, 15 Jan 2022 21:48:21: 3000000 INFO @ Sat, 15 Jan 2022 21:48:27: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:33: 5000000 INFO @ Sat, 15 Jan 2022 21:48:38: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:48:38: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:48:38: #1 total tags in treatment: 2339783 INFO @ Sat, 15 Jan 2022 21:48:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:48:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:48:38: #1 tags after filtering in treatment: 2071476 INFO @ Sat, 15 Jan 2022 21:48:38: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 15 Jan 2022 21:48:38: #1 finished! INFO @ Sat, 15 Jan 2022 21:48:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:48:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:48:38: #2 number of paired peaks: 98 WARNING @ Sat, 15 Jan 2022 21:48:38: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:48:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:48:39: 1000000 INFO @ Sat, 15 Jan 2022 21:48:45: 2000000 INFO @ Sat, 15 Jan 2022 21:48:50: 3000000 INFO @ Sat, 15 Jan 2022 21:48:56: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:49:02: 5000000 INFO @ Sat, 15 Jan 2022 21:49:06: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 21:49:06: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 21:49:06: #1 total tags in treatment: 2339783 INFO @ Sat, 15 Jan 2022 21:49:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:06: #1 tags after filtering in treatment: 2071476 INFO @ Sat, 15 Jan 2022 21:49:06: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 15 Jan 2022 21:49:06: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:06: #2 number of paired peaks: 98 WARNING @ Sat, 15 Jan 2022 21:49:06: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4439542/ERX4439542.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。