Job ID = 2007741 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-07-05T07:25:10 fasterq-dump.2.9.6 err: index.c get_nearest_offset() -> too many rounds pos = 24 of 40 spots read : 13,771 reads read : 27,542 reads written : 27,542 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 13771 reads; of these: 13771 (100.00%) were paired; of these: 967 (7.02%) aligned concordantly 0 times 11614 (84.34%) aligned concordantly exactly 1 time 1190 (8.64%) aligned concordantly >1 times ---- 967 pairs aligned concordantly 0 times; of these: 246 (25.44%) aligned discordantly 1 time ---- 721 pairs aligned 0 times concordantly or discordantly; of these: 1442 mates make up the pairs; of these: 1232 (85.44%) aligned 0 times 130 (9.02%) aligned exactly 1 time 80 (5.55%) aligned >1 times 95.53% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 14 / 12970 = 0.0011 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 16:25:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:25:27: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:25:27: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:25:27: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:25:27: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:25:27: #1 total tags in treatment: 12790 INFO @ Fri, 05 Jul 2019 16:25:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:25:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:25:27: #1 tags after filtering in treatment: 12780 INFO @ Fri, 05 Jul 2019 16:25:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:25:27: #1 finished! INFO @ Fri, 05 Jul 2019 16:25:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:25:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:25:27: #2 number of paired peaks: 160 WARNING @ Fri, 05 Jul 2019 16:25:27: Fewer paired peaks (160) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 160 pairs to build model! INFO @ Fri, 05 Jul 2019 16:25:27: start model_add_line... INFO @ Fri, 05 Jul 2019 16:25:27: start X-correlation... INFO @ Fri, 05 Jul 2019 16:25:27: end of X-cor INFO @ Fri, 05 Jul 2019 16:25:27: #2 finished! INFO @ Fri, 05 Jul 2019 16:25:27: #2 predicted fragment length is 276 bps INFO @ Fri, 05 Jul 2019 16:25:27: #2 alternative fragment length(s) may be 4,30,54,77,115,133,169,188,217,230,256,276,301,315,358,395,416,459,527,547,597 bps INFO @ Fri, 05 Jul 2019 16:25:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.05_model.r INFO @ Fri, 05 Jul 2019 16:25:27: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:25:27: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:25:27: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:25:27: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.05_peaks.xls INFO @ Fri, 05 Jul 2019 16:25:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:25:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.05_summits.bed INFO @ Fri, 05 Jul 2019 16:25:27: Done! BigWig に変換しました。 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:25:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:25:28: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:25:28: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:25:28: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:25:28: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:25:28: #1 total tags in treatment: 12790 INFO @ Fri, 05 Jul 2019 16:25:28: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:25:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:25:28: #1 tags after filtering in treatment: 12780 INFO @ Fri, 05 Jul 2019 16:25:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:25:28: #1 finished! INFO @ Fri, 05 Jul 2019 16:25:28: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:25:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:25:28: #2 number of paired peaks: 160 WARNING @ Fri, 05 Jul 2019 16:25:28: Fewer paired peaks (160) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 160 pairs to build model! INFO @ Fri, 05 Jul 2019 16:25:28: start model_add_line... INFO @ Fri, 05 Jul 2019 16:25:28: start X-correlation... INFO @ Fri, 05 Jul 2019 16:25:28: end of X-cor INFO @ Fri, 05 Jul 2019 16:25:28: #2 finished! INFO @ Fri, 05 Jul 2019 16:25:28: #2 predicted fragment length is 276 bps INFO @ Fri, 05 Jul 2019 16:25:28: #2 alternative fragment length(s) may be 4,30,54,77,115,133,169,188,217,230,256,276,301,315,358,395,416,459,527,547,597 bps INFO @ Fri, 05 Jul 2019 16:25:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.10_model.r INFO @ Fri, 05 Jul 2019 16:25:28: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:25:28: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:25:28: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:25:28: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.10_peaks.xls INFO @ Fri, 05 Jul 2019 16:25:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:25:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.10_summits.bed INFO @ Fri, 05 Jul 2019 16:25:28: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:25:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:25:29: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:25:29: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:25:29: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:25:29: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:25:29: #1 total tags in treatment: 12790 INFO @ Fri, 05 Jul 2019 16:25:29: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:25:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:25:29: #1 tags after filtering in treatment: 12780 INFO @ Fri, 05 Jul 2019 16:25:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:25:29: #1 finished! INFO @ Fri, 05 Jul 2019 16:25:29: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:25:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:25:29: #2 number of paired peaks: 160 WARNING @ Fri, 05 Jul 2019 16:25:29: Fewer paired peaks (160) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 160 pairs to build model! INFO @ Fri, 05 Jul 2019 16:25:29: start model_add_line... INFO @ Fri, 05 Jul 2019 16:25:29: start X-correlation... INFO @ Fri, 05 Jul 2019 16:25:29: end of X-cor INFO @ Fri, 05 Jul 2019 16:25:29: #2 finished! INFO @ Fri, 05 Jul 2019 16:25:29: #2 predicted fragment length is 276 bps INFO @ Fri, 05 Jul 2019 16:25:29: #2 alternative fragment length(s) may be 4,30,54,77,115,133,169,188,217,230,256,276,301,315,358,395,416,459,527,547,597 bps INFO @ Fri, 05 Jul 2019 16:25:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.20_model.r INFO @ Fri, 05 Jul 2019 16:25:29: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:25:29: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:25:29: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:25:29: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.20_peaks.xls INFO @ Fri, 05 Jul 2019 16:25:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:25:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX433686/ERX433686.20_summits.bed INFO @ Fri, 05 Jul 2019 16:25:29: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling