Job ID = 2007728 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 3,373 reads read : 6,746 reads written : 6,746 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR467441.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 3373 reads; of these: 3373 (100.00%) were paired; of these: 697 (20.66%) aligned concordantly 0 times 2429 (72.01%) aligned concordantly exactly 1 time 247 (7.32%) aligned concordantly >1 times ---- 697 pairs aligned concordantly 0 times; of these: 50 (7.17%) aligned discordantly 1 time ---- 647 pairs aligned 0 times concordantly or discordantly; of these: 1294 mates make up the pairs; of these: 1237 (95.60%) aligned 0 times 35 (2.70%) aligned exactly 1 time 22 (1.70%) aligned >1 times 81.66% overall alignment rate Time searching: 00:00:00 Overall time: 00:00:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 7 / 2720 = 0.0026 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Fri, 05 Jul 2019 16:23:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:23:20: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:23:20: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:23:20: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:23:20: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:23:20: #1 total tags in treatment: 2669 INFO @ Fri, 05 Jul 2019 16:23:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:23:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:23:20: #1 tags after filtering in treatment: 2669 INFO @ Fri, 05 Jul 2019 16:23:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:23:20: #1 finished! INFO @ Fri, 05 Jul 2019 16:23:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:23:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:23:20: #2 number of paired peaks: 92 WARNING @ Fri, 05 Jul 2019 16:23:20: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:23:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:23:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:23:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:23:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:23:21: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:23:21: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:23:21: #1 total tags in treatment: 2669 INFO @ Fri, 05 Jul 2019 16:23:21: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:23:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:23:21: #1 tags after filtering in treatment: 2669 INFO @ Fri, 05 Jul 2019 16:23:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:23:21: #1 finished! INFO @ Fri, 05 Jul 2019 16:23:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:23:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:23:21: #2 number of paired peaks: 92 WARNING @ Fri, 05 Jul 2019 16:23:21: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:23:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:23:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:23:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:23:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:23:22: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:23:22: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:23:22: #1 total tags in treatment: 2669 INFO @ Fri, 05 Jul 2019 16:23:22: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:23:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:23:22: #1 tags after filtering in treatment: 2669 INFO @ Fri, 05 Jul 2019 16:23:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:23:22: #1 finished! INFO @ Fri, 05 Jul 2019 16:23:22: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:23:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:23:22: #2 number of paired peaks: 92 WARNING @ Fri, 05 Jul 2019 16:23:22: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:23:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX433676/ERX433676.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling