Job ID = 14521056 SRX = ERX4123676 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 12685874 spots for ERR4157931/ERR4157931.sra Written 12685874 spots for ERR4157931/ERR4157931.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:18 12685874 reads; of these: 12685874 (100.00%) were unpaired; of these: 170699 (1.35%) aligned 0 times 10329430 (81.42%) aligned exactly 1 time 2185745 (17.23%) aligned >1 times 98.65% overall alignment rate Time searching: 00:02:18 Overall time: 00:02:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6129468 / 12515175 = 0.4898 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:25:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:25:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:25:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:05: 1000000 INFO @ Sat, 15 Jan 2022 20:26:10: 2000000 INFO @ Sat, 15 Jan 2022 20:26:15: 3000000 INFO @ Sat, 15 Jan 2022 20:26:20: 4000000 INFO @ Sat, 15 Jan 2022 20:26:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:31: 6000000 INFO @ Sat, 15 Jan 2022 20:26:33: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:33: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:33: #1 total tags in treatment: 6385707 INFO @ Sat, 15 Jan 2022 20:26:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:34: #1 tags after filtering in treatment: 6385707 INFO @ Sat, 15 Jan 2022 20:26:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:26:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:26:34: 1000000 INFO @ Sat, 15 Jan 2022 20:26:39: 2000000 INFO @ Sat, 15 Jan 2022 20:26:44: 3000000 INFO @ Sat, 15 Jan 2022 20:26:48: 4000000 INFO @ Sat, 15 Jan 2022 20:26:53: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:58: 6000000 INFO @ Sat, 15 Jan 2022 20:26:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:26:59: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:59: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:59: #1 total tags in treatment: 6385707 INFO @ Sat, 15 Jan 2022 20:26:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:59: #1 tags after filtering in treatment: 6385707 INFO @ Sat, 15 Jan 2022 20:26:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:26:59: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:27:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:27:04: 1000000 INFO @ Sat, 15 Jan 2022 20:27:10: 2000000 INFO @ Sat, 15 Jan 2022 20:27:15: 3000000 INFO @ Sat, 15 Jan 2022 20:27:21: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:27:26: 5000000 INFO @ Sat, 15 Jan 2022 20:27:31: 6000000 INFO @ Sat, 15 Jan 2022 20:27:33: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:27:33: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:27:33: #1 total tags in treatment: 6385707 INFO @ Sat, 15 Jan 2022 20:27:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:34: #1 tags after filtering in treatment: 6385707 INFO @ Sat, 15 Jan 2022 20:27:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:27:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:27:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123676/ERX4123676.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。