Job ID = 14520991 SRX = ERX4123669 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 12863987 spots for ERR4157924/ERR4157924.sra Written 12863987 spots for ERR4157924/ERR4157924.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:31 12863987 reads; of these: 12863987 (100.00%) were unpaired; of these: 3214742 (24.99%) aligned 0 times 4707383 (36.59%) aligned exactly 1 time 4941862 (38.42%) aligned >1 times 75.01% overall alignment rate Time searching: 00:01:31 Overall time: 00:01:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 7877254 / 9649245 = 0.8164 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:17:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:17:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:17:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:17:10: 1000000 INFO @ Sat, 15 Jan 2022 20:17:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:17:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:17:14: #1 total tags in treatment: 1771991 INFO @ Sat, 15 Jan 2022 20:17:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:14: #1 tags after filtering in treatment: 1771991 INFO @ Sat, 15 Jan 2022 20:17:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:17:14: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:15: #2 number of paired peaks: 296 WARNING @ Sat, 15 Jan 2022 20:17:15: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Sat, 15 Jan 2022 20:17:15: start model_add_line... INFO @ Sat, 15 Jan 2022 20:17:15: start X-correlation... INFO @ Sat, 15 Jan 2022 20:17:15: end of X-cor INFO @ Sat, 15 Jan 2022 20:17:15: #2 finished! INFO @ Sat, 15 Jan 2022 20:17:15: #2 predicted fragment length is 182 bps INFO @ Sat, 15 Jan 2022 20:17:15: #2 alternative fragment length(s) may be 182 bps INFO @ Sat, 15 Jan 2022 20:17:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.05_model.r INFO @ Sat, 15 Jan 2022 20:17:15: #3 Call peaks... INFO @ Sat, 15 Jan 2022 20:17:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 20:17:20: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 20:17:21: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.05_peaks.xls INFO @ Sat, 15 Jan 2022 20:17:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.05_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 20:17:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.05_summits.bed INFO @ Sat, 15 Jan 2022 20:17:21: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (818 records, 4 fields): 3 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:17:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:17:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:17:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:17:39: 1000000 INFO @ Sat, 15 Jan 2022 20:17:44: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:17:44: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:17:44: #1 total tags in treatment: 1771991 INFO @ Sat, 15 Jan 2022 20:17:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:44: #1 tags after filtering in treatment: 1771991 INFO @ Sat, 15 Jan 2022 20:17:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:17:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:44: #2 number of paired peaks: 296 WARNING @ Sat, 15 Jan 2022 20:17:44: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Sat, 15 Jan 2022 20:17:44: start model_add_line... INFO @ Sat, 15 Jan 2022 20:17:44: start X-correlation... INFO @ Sat, 15 Jan 2022 20:17:44: end of X-cor INFO @ Sat, 15 Jan 2022 20:17:44: #2 finished! INFO @ Sat, 15 Jan 2022 20:17:44: #2 predicted fragment length is 182 bps INFO @ Sat, 15 Jan 2022 20:17:44: #2 alternative fragment length(s) may be 182 bps INFO @ Sat, 15 Jan 2022 20:17:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.10_model.r INFO @ Sat, 15 Jan 2022 20:17:44: #3 Call peaks... INFO @ Sat, 15 Jan 2022 20:17:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 20:17:49: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 20:17:50: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.10_peaks.xls INFO @ Sat, 15 Jan 2022 20:17:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.10_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 20:17:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.10_summits.bed INFO @ Sat, 15 Jan 2022 20:17:50: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (617 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:18:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:18:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:18:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:18:10: 1000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:18:14: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:18:14: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:18:14: #1 total tags in treatment: 1771991 INFO @ Sat, 15 Jan 2022 20:18:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:18:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:18:14: #1 tags after filtering in treatment: 1771991 INFO @ Sat, 15 Jan 2022 20:18:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:18:14: #1 finished! INFO @ Sat, 15 Jan 2022 20:18:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:18:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:18:14: #2 number of paired peaks: 296 WARNING @ Sat, 15 Jan 2022 20:18:14: Fewer paired peaks (296) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 296 pairs to build model! INFO @ Sat, 15 Jan 2022 20:18:14: start model_add_line... INFO @ Sat, 15 Jan 2022 20:18:14: start X-correlation... INFO @ Sat, 15 Jan 2022 20:18:14: end of X-cor INFO @ Sat, 15 Jan 2022 20:18:14: #2 finished! INFO @ Sat, 15 Jan 2022 20:18:14: #2 predicted fragment length is 182 bps INFO @ Sat, 15 Jan 2022 20:18:14: #2 alternative fragment length(s) may be 182 bps INFO @ Sat, 15 Jan 2022 20:18:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.20_model.r INFO @ Sat, 15 Jan 2022 20:18:14: #3 Call peaks... INFO @ Sat, 15 Jan 2022 20:18:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 20:18:20: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 20:18:21: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.20_peaks.xls INFO @ Sat, 15 Jan 2022 20:18:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.20_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 20:18:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4123669/ERX4123669.20_summits.bed INFO @ Sat, 15 Jan 2022 20:18:21: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (450 records, 4 fields): 2 millis CompletedMACS2peakCalling