Job ID = 14520989 SRX = ERX4123667 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10964801 spots for ERR4157922/ERR4157922.sra Written 10964801 spots for ERR4157922/ERR4157922.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:07 10964801 reads; of these: 10964801 (100.00%) were unpaired; of these: 135839 (1.24%) aligned 0 times 8929161 (81.43%) aligned exactly 1 time 1899801 (17.33%) aligned >1 times 98.76% overall alignment rate Time searching: 00:02:07 Overall time: 00:02:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5097629 / 10828962 = 0.4707 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:17:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:17:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:17:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:17:56: 1000000 INFO @ Sat, 15 Jan 2022 20:18:01: 2000000 INFO @ Sat, 15 Jan 2022 20:18:06: 3000000 INFO @ Sat, 15 Jan 2022 20:18:11: 4000000 INFO @ Sat, 15 Jan 2022 20:18:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:18:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:18:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:18:20: #1 total tags in treatment: 5731333 INFO @ Sat, 15 Jan 2022 20:18:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:18:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:18:20: #1 tags after filtering in treatment: 5731333 INFO @ Sat, 15 Jan 2022 20:18:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:18:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:18:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:18:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:18:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:18:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:18:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:18:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:18:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:18:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:18:26: 1000000 INFO @ Sat, 15 Jan 2022 20:18:31: 2000000 INFO @ Sat, 15 Jan 2022 20:18:36: 3000000 INFO @ Sat, 15 Jan 2022 20:18:42: 4000000 INFO @ Sat, 15 Jan 2022 20:18:47: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:18:51: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:18:51: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:18:51: #1 total tags in treatment: 5731333 INFO @ Sat, 15 Jan 2022 20:18:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:18:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:18:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:18:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:18:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:18:51: #1 tags after filtering in treatment: 5731333 INFO @ Sat, 15 Jan 2022 20:18:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:18:51: #1 finished! INFO @ Sat, 15 Jan 2022 20:18:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:18:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:18:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:18:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:18:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:18:56: 1000000 INFO @ Sat, 15 Jan 2022 20:19:01: 2000000 INFO @ Sat, 15 Jan 2022 20:19:06: 3000000 INFO @ Sat, 15 Jan 2022 20:19:11: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:19:16: 5000000 INFO @ Sat, 15 Jan 2022 20:19:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:19:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:19:20: #1 total tags in treatment: 5731333 INFO @ Sat, 15 Jan 2022 20:19:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:20: #1 tags after filtering in treatment: 5731333 INFO @ Sat, 15 Jan 2022 20:19:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:19:20: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4123667/ERX4123667.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。