Job ID = 14519826 SRX = ERX4107475 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42259604 spots for ERR4140184/ERR4140184.sra Written 42259604 spots for ERR4140184/ERR4140184.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:03 42259604 reads; of these: 42259604 (100.00%) were unpaired; of these: 326727 (0.77%) aligned 0 times 36823234 (87.14%) aligned exactly 1 time 5109643 (12.09%) aligned >1 times 99.23% overall alignment rate Time searching: 00:07:03 Overall time: 00:07:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 28315751 / 41932877 = 0.6753 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:05:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:05:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:05:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:05:11: 1000000 INFO @ Sat, 15 Jan 2022 18:05:16: 2000000 INFO @ Sat, 15 Jan 2022 18:05:23: 3000000 INFO @ Sat, 15 Jan 2022 18:05:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:05:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:05:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:05:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:05:34: 5000000 INFO @ Sat, 15 Jan 2022 18:05:40: 1000000 INFO @ Sat, 15 Jan 2022 18:05:41: 6000000 INFO @ Sat, 15 Jan 2022 18:05:46: 2000000 INFO @ Sat, 15 Jan 2022 18:05:47: 7000000 INFO @ Sat, 15 Jan 2022 18:05:53: 3000000 INFO @ Sat, 15 Jan 2022 18:05:53: 8000000 INFO @ Sat, 15 Jan 2022 18:05:59: 4000000 INFO @ Sat, 15 Jan 2022 18:05:59: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:06:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:06:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:06:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:06:05: 5000000 INFO @ Sat, 15 Jan 2022 18:06:06: 10000000 INFO @ Sat, 15 Jan 2022 18:06:11: 1000000 INFO @ Sat, 15 Jan 2022 18:06:12: 6000000 INFO @ Sat, 15 Jan 2022 18:06:13: 11000000 INFO @ Sat, 15 Jan 2022 18:06:18: 2000000 INFO @ Sat, 15 Jan 2022 18:06:19: 12000000 INFO @ Sat, 15 Jan 2022 18:06:19: 7000000 INFO @ Sat, 15 Jan 2022 18:06:25: 3000000 INFO @ Sat, 15 Jan 2022 18:06:25: 13000000 INFO @ Sat, 15 Jan 2022 18:06:27: 8000000 INFO @ Sat, 15 Jan 2022 18:06:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:06:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:06:29: #1 total tags in treatment: 13617126 INFO @ Sat, 15 Jan 2022 18:06:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:06:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:06:29: #1 tags after filtering in treatment: 13617126 INFO @ Sat, 15 Jan 2022 18:06:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:06:29: #1 finished! INFO @ Sat, 15 Jan 2022 18:06:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:06:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:06:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:06:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:06:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:06:32: 4000000 INFO @ Sat, 15 Jan 2022 18:06:33: 9000000 INFO @ Sat, 15 Jan 2022 18:06:38: 5000000 INFO @ Sat, 15 Jan 2022 18:06:40: 10000000 INFO @ Sat, 15 Jan 2022 18:06:45: 6000000 INFO @ Sat, 15 Jan 2022 18:06:46: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:06:51: 7000000 INFO @ Sat, 15 Jan 2022 18:06:53: 12000000 INFO @ Sat, 15 Jan 2022 18:06:58: 8000000 INFO @ Sat, 15 Jan 2022 18:06:59: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:07:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:07:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:07:03: #1 total tags in treatment: 13617126 INFO @ Sat, 15 Jan 2022 18:07:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:07:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:07:03: #1 tags after filtering in treatment: 13617126 INFO @ Sat, 15 Jan 2022 18:07:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:07:03: #1 finished! INFO @ Sat, 15 Jan 2022 18:07:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:07:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:07:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:07:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:07:04: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 18:07:04: 9000000 cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:07:10: 10000000 INFO @ Sat, 15 Jan 2022 18:07:16: 11000000 INFO @ Sat, 15 Jan 2022 18:07:22: 12000000 INFO @ Sat, 15 Jan 2022 18:07:28: 13000000 INFO @ Sat, 15 Jan 2022 18:07:32: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:07:32: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:07:32: #1 total tags in treatment: 13617126 INFO @ Sat, 15 Jan 2022 18:07:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:07:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:07:32: #1 tags after filtering in treatment: 13617126 INFO @ Sat, 15 Jan 2022 18:07:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:07:32: #1 finished! INFO @ Sat, 15 Jan 2022 18:07:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:07:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:07:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:07:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:07:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107475/ERX4107475.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling