Job ID = 14519825 SRX = ERX4107474 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13709318 spots for ERR4140183/ERR4140183.sra Written 13709318 spots for ERR4140183/ERR4140183.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:15 13709318 reads; of these: 13709318 (100.00%) were unpaired; of these: 178654 (1.30%) aligned 0 times 11415508 (83.27%) aligned exactly 1 time 2115156 (15.43%) aligned >1 times 98.70% overall alignment rate Time searching: 00:02:15 Overall time: 00:02:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7178268 / 13530664 = 0.5305 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:54:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:54:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:54:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:54:54: 1000000 INFO @ Sat, 15 Jan 2022 17:54:59: 2000000 INFO @ Sat, 15 Jan 2022 17:55:04: 3000000 INFO @ Sat, 15 Jan 2022 17:55:08: 4000000 INFO @ Sat, 15 Jan 2022 17:55:13: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:55:18: 6000000 INFO @ Sat, 15 Jan 2022 17:55:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:55:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:55:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:55:20: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:55:20: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:55:20: #1 total tags in treatment: 6352396 INFO @ Sat, 15 Jan 2022 17:55:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:55:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:55:20: #1 tags after filtering in treatment: 6352396 INFO @ Sat, 15 Jan 2022 17:55:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 17:55:20: #1 finished! INFO @ Sat, 15 Jan 2022 17:55:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:55:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:55:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:55:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:55:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:55:24: 1000000 INFO @ Sat, 15 Jan 2022 17:55:30: 2000000 INFO @ Sat, 15 Jan 2022 17:55:36: 3000000 INFO @ Sat, 15 Jan 2022 17:55:42: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:55:48: 5000000 INFO @ Sat, 15 Jan 2022 17:55:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:55:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:55:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:55:54: 6000000 INFO @ Sat, 15 Jan 2022 17:55:55: 1000000 INFO @ Sat, 15 Jan 2022 17:55:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:55:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:55:56: #1 total tags in treatment: 6352396 INFO @ Sat, 15 Jan 2022 17:55:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:55:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:55:56: #1 tags after filtering in treatment: 6352396 INFO @ Sat, 15 Jan 2022 17:55:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 17:55:56: #1 finished! INFO @ Sat, 15 Jan 2022 17:55:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:55:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:55:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:55:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:55:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:56:01: 2000000 INFO @ Sat, 15 Jan 2022 17:56:06: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:56:11: 4000000 INFO @ Sat, 15 Jan 2022 17:56:16: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:56:20: 6000000 INFO @ Sat, 15 Jan 2022 17:56:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:56:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:56:22: #1 total tags in treatment: 6352396 INFO @ Sat, 15 Jan 2022 17:56:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:56:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:56:22: #1 tags after filtering in treatment: 6352396 INFO @ Sat, 15 Jan 2022 17:56:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 17:56:22: #1 finished! INFO @ Sat, 15 Jan 2022 17:56:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:56:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:56:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:56:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:56:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107474/ERX4107474.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling