Job ID = 14519797 SRX = ERX4107469 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 43698461 spots for ERR4140178/ERR4140178.sra Written 43698461 spots for ERR4140178/ERR4140178.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:30 43698461 reads; of these: 43698461 (100.00%) were unpaired; of these: 404783 (0.93%) aligned 0 times 38218417 (87.46%) aligned exactly 1 time 5075261 (11.61%) aligned >1 times 99.07% overall alignment rate Time searching: 00:05:30 Overall time: 00:05:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 29204216 / 43293678 = 0.6746 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:59:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:59:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:59:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:59:32: 1000000 INFO @ Sat, 15 Jan 2022 17:59:38: 2000000 INFO @ Sat, 15 Jan 2022 17:59:45: 3000000 INFO @ Sat, 15 Jan 2022 17:59:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:59:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:59:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:59:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:59:57: 5000000 INFO @ Sat, 15 Jan 2022 18:00:02: 1000000 INFO @ Sat, 15 Jan 2022 18:00:05: 6000000 INFO @ Sat, 15 Jan 2022 18:00:08: 2000000 INFO @ Sat, 15 Jan 2022 18:00:12: 7000000 INFO @ Sat, 15 Jan 2022 18:00:15: 3000000 INFO @ Sat, 15 Jan 2022 18:00:19: 8000000 INFO @ Sat, 15 Jan 2022 18:00:21: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:00:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:00:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:00:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:00:26: 9000000 INFO @ Sat, 15 Jan 2022 18:00:27: 5000000 INFO @ Sat, 15 Jan 2022 18:00:33: 1000000 INFO @ Sat, 15 Jan 2022 18:00:34: 10000000 INFO @ Sat, 15 Jan 2022 18:00:34: 6000000 INFO @ Sat, 15 Jan 2022 18:00:40: 2000000 INFO @ Sat, 15 Jan 2022 18:00:40: 7000000 INFO @ Sat, 15 Jan 2022 18:00:41: 11000000 INFO @ Sat, 15 Jan 2022 18:00:47: 8000000 INFO @ Sat, 15 Jan 2022 18:00:47: 3000000 INFO @ Sat, 15 Jan 2022 18:00:48: 12000000 INFO @ Sat, 15 Jan 2022 18:00:53: 9000000 INFO @ Sat, 15 Jan 2022 18:00:54: 4000000 INFO @ Sat, 15 Jan 2022 18:00:56: 13000000 INFO @ Sat, 15 Jan 2022 18:00:59: 10000000 INFO @ Sat, 15 Jan 2022 18:01:02: 5000000 INFO @ Sat, 15 Jan 2022 18:01:03: 14000000 INFO @ Sat, 15 Jan 2022 18:01:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:01:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:01:03: #1 total tags in treatment: 14089462 INFO @ Sat, 15 Jan 2022 18:01:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:01:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:01:04: #1 tags after filtering in treatment: 14089462 INFO @ Sat, 15 Jan 2022 18:01:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:01:04: #1 finished! INFO @ Sat, 15 Jan 2022 18:01:04: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:01:04: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:01:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:01:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:01:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:01:06: 11000000 INFO @ Sat, 15 Jan 2022 18:01:08: 6000000 INFO @ Sat, 15 Jan 2022 18:01:12: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:01:15: 7000000 INFO @ Sat, 15 Jan 2022 18:01:18: 13000000 INFO @ Sat, 15 Jan 2022 18:01:22: 8000000 INFO @ Sat, 15 Jan 2022 18:01:24: 14000000 INFO @ Sat, 15 Jan 2022 18:01:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:01:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:01:25: #1 total tags in treatment: 14089462 INFO @ Sat, 15 Jan 2022 18:01:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:01:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:01:25: #1 tags after filtering in treatment: 14089462 INFO @ Sat, 15 Jan 2022 18:01:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:01:25: #1 finished! INFO @ Sat, 15 Jan 2022 18:01:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:01:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:01:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:01:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:01:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:01:29: 9000000 INFO @ Sat, 15 Jan 2022 18:01:36: 10000000 INFO @ Sat, 15 Jan 2022 18:01:42: 11000000 INFO @ Sat, 15 Jan 2022 18:01:48: 12000000 INFO @ Sat, 15 Jan 2022 18:01:55: 13000000 INFO @ Sat, 15 Jan 2022 18:02:01: 14000000 INFO @ Sat, 15 Jan 2022 18:02:02: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:02:02: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:02:02: #1 total tags in treatment: 14089462 INFO @ Sat, 15 Jan 2022 18:02:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:02:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:02:02: #1 tags after filtering in treatment: 14089462 INFO @ Sat, 15 Jan 2022 18:02:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:02:02: #1 finished! INFO @ Sat, 15 Jan 2022 18:02:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:02:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:02:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:02:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:02:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107469/ERX4107469.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling