Job ID = 14519790 SRX = ERX4107464 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 43668429 spots for ERR4140173/ERR4140173.sra Written 43668429 spots for ERR4140173/ERR4140173.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:50 43668429 reads; of these: 43668429 (100.00%) were unpaired; of these: 459249 (1.05%) aligned 0 times 37381354 (85.60%) aligned exactly 1 time 5827826 (13.35%) aligned >1 times 98.95% overall alignment rate Time searching: 00:06:50 Overall time: 00:06:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 29247872 / 43209180 = 0.6769 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:00:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:00:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:00:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:00:58: 1000000 INFO @ Sat, 15 Jan 2022 18:01:03: 2000000 INFO @ Sat, 15 Jan 2022 18:01:07: 3000000 INFO @ Sat, 15 Jan 2022 18:01:12: 4000000 INFO @ Sat, 15 Jan 2022 18:01:17: 5000000 INFO @ Sat, 15 Jan 2022 18:01:21: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:01:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:01:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:01:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:01:26: 7000000 INFO @ Sat, 15 Jan 2022 18:01:28: 1000000 INFO @ Sat, 15 Jan 2022 18:01:31: 8000000 INFO @ Sat, 15 Jan 2022 18:01:33: 2000000 INFO @ Sat, 15 Jan 2022 18:01:35: 9000000 INFO @ Sat, 15 Jan 2022 18:01:38: 3000000 INFO @ Sat, 15 Jan 2022 18:01:40: 10000000 INFO @ Sat, 15 Jan 2022 18:01:42: 4000000 INFO @ Sat, 15 Jan 2022 18:01:45: 11000000 INFO @ Sat, 15 Jan 2022 18:01:47: 5000000 INFO @ Sat, 15 Jan 2022 18:01:50: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:01:52: 6000000 INFO @ Sat, 15 Jan 2022 18:01:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:01:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:01:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:01:54: 13000000 INFO @ Sat, 15 Jan 2022 18:01:57: 7000000 INFO @ Sat, 15 Jan 2022 18:01:59: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:01:59: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:01:59: #1 total tags in treatment: 13961308 INFO @ Sat, 15 Jan 2022 18:01:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:01:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:01:59: 1000000 INFO @ Sat, 15 Jan 2022 18:02:00: #1 tags after filtering in treatment: 13961308 INFO @ Sat, 15 Jan 2022 18:02:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:02:00: #1 finished! INFO @ Sat, 15 Jan 2022 18:02:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:02:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:02:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:02:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:02:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:02:02: 8000000 INFO @ Sat, 15 Jan 2022 18:02:05: 2000000 INFO @ Sat, 15 Jan 2022 18:02:07: 9000000 INFO @ Sat, 15 Jan 2022 18:02:10: 3000000 INFO @ Sat, 15 Jan 2022 18:02:11: 10000000 INFO @ Sat, 15 Jan 2022 18:02:16: 4000000 INFO @ Sat, 15 Jan 2022 18:02:16: 11000000 INFO @ Sat, 15 Jan 2022 18:02:21: 12000000 INFO @ Sat, 15 Jan 2022 18:02:21: 5000000 INFO @ Sat, 15 Jan 2022 18:02:26: 13000000 INFO @ Sat, 15 Jan 2022 18:02:27: 6000000 INFO @ Sat, 15 Jan 2022 18:02:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:02:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:02:31: #1 total tags in treatment: 13961308 INFO @ Sat, 15 Jan 2022 18:02:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:02:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:02:31: #1 tags after filtering in treatment: 13961308 INFO @ Sat, 15 Jan 2022 18:02:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:02:31: #1 finished! INFO @ Sat, 15 Jan 2022 18:02:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:02:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:02:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:02:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:02:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.10_peaks.narrowPeak BedGraph に変換しました。 : No such file or directory BigWig に変換中... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:02:32: 7000000 INFO @ Sat, 15 Jan 2022 18:02:38: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:02:43: 9000000 INFO @ Sat, 15 Jan 2022 18:02:48: 10000000 INFO @ Sat, 15 Jan 2022 18:02:54: 11000000 INFO @ Sat, 15 Jan 2022 18:02:59: 12000000 INFO @ Sat, 15 Jan 2022 18:03:04: 13000000 INFO @ Sat, 15 Jan 2022 18:03:10: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 18:03:10: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 18:03:10: #1 total tags in treatment: 13961308 INFO @ Sat, 15 Jan 2022 18:03:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:03:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:03:10: #1 tags after filtering in treatment: 13961308 INFO @ Sat, 15 Jan 2022 18:03:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:03:10: #1 finished! INFO @ Sat, 15 Jan 2022 18:03:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:03:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:03:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:03:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:03:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX4107464/ERX4107464.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling