Job ID = 2007661 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,894,745 reads read : 21,894,745 reads written : 21,894,745 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:11 21894745 reads; of these: 21894745 (100.00%) were unpaired; of these: 1760295 (8.04%) aligned 0 times 17426703 (79.59%) aligned exactly 1 time 2707747 (12.37%) aligned >1 times 91.96% overall alignment rate Time searching: 00:06:11 Overall time: 00:06:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 11420194 / 20134450 = 0.5672 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 16:28:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:28:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:28:40: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:28:40: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:28:40: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:28:40: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:28:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:28:41: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:28:41: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:28:48: 1000000 INFO @ Fri, 05 Jul 2019 16:28:49: 1000000 INFO @ Fri, 05 Jul 2019 16:28:49: 1000000 INFO @ Fri, 05 Jul 2019 16:28:56: 2000000 INFO @ Fri, 05 Jul 2019 16:28:58: 2000000 INFO @ Fri, 05 Jul 2019 16:28:58: 2000000 INFO @ Fri, 05 Jul 2019 16:29:03: 3000000 INFO @ Fri, 05 Jul 2019 16:29:06: 3000000 INFO @ Fri, 05 Jul 2019 16:29:07: 3000000 INFO @ Fri, 05 Jul 2019 16:29:10: 4000000 INFO @ Fri, 05 Jul 2019 16:29:14: 4000000 INFO @ Fri, 05 Jul 2019 16:29:15: 4000000 INFO @ Fri, 05 Jul 2019 16:29:17: 5000000 INFO @ Fri, 05 Jul 2019 16:29:23: 5000000 INFO @ Fri, 05 Jul 2019 16:29:24: 5000000 INFO @ Fri, 05 Jul 2019 16:29:24: 6000000 INFO @ Fri, 05 Jul 2019 16:29:31: 6000000 INFO @ Fri, 05 Jul 2019 16:29:32: 7000000 INFO @ Fri, 05 Jul 2019 16:29:33: 6000000 INFO @ Fri, 05 Jul 2019 16:29:39: 8000000 INFO @ Fri, 05 Jul 2019 16:29:40: 7000000 INFO @ Fri, 05 Jul 2019 16:29:41: 7000000 INFO @ Fri, 05 Jul 2019 16:29:44: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 16:29:44: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 16:29:44: #1 total tags in treatment: 8714256 INFO @ Fri, 05 Jul 2019 16:29:44: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:29:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:29:44: #1 tags after filtering in treatment: 8714256 INFO @ Fri, 05 Jul 2019 16:29:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:29:44: #1 finished! INFO @ Fri, 05 Jul 2019 16:29:44: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:29:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:29:45: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 16:29:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:29:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 42 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:29:48: 8000000 INFO @ Fri, 05 Jul 2019 16:29:50: 8000000 INFO @ Fri, 05 Jul 2019 16:29:54: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 16:29:54: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 16:29:54: #1 total tags in treatment: 8714256 INFO @ Fri, 05 Jul 2019 16:29:54: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:29:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:29:54: #1 tags after filtering in treatment: 8714256 INFO @ Fri, 05 Jul 2019 16:29:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:29:54: #1 finished! INFO @ Fri, 05 Jul 2019 16:29:54: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:29:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:29:55: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 16:29:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:29:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:29:56: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 16:29:56: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 16:29:56: #1 total tags in treatment: 8714256 INFO @ Fri, 05 Jul 2019 16:29:56: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:29:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:29:56: #1 tags after filtering in treatment: 8714256 INFO @ Fri, 05 Jul 2019 16:29:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:29:56: #1 finished! INFO @ Fri, 05 Jul 2019 16:29:56: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:29:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:29:57: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 16:29:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 16:29:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX291904/ERX291904.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。