Job ID = 2640742 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 31,137,525 reads read : 31,137,525 reads written : 31,137,525 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:49 31137525 reads; of these: 31137525 (100.00%) were unpaired; of these: 284879 (0.91%) aligned 0 times 26838959 (86.19%) aligned exactly 1 time 4013687 (12.89%) aligned >1 times 99.09% overall alignment rate Time searching: 00:07:49 Overall time: 00:07:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15882433 / 30852646 = 0.5148 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:15:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:15:22: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:15:22: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:15:30: 1000000 INFO @ Sat, 24 Aug 2019 19:15:37: 2000000 INFO @ Sat, 24 Aug 2019 19:15:44: 3000000 INFO @ Sat, 24 Aug 2019 19:15:51: 4000000 INFO @ Sat, 24 Aug 2019 19:15:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:15:52: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:15:52: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:15:58: 5000000 INFO @ Sat, 24 Aug 2019 19:16:00: 1000000 INFO @ Sat, 24 Aug 2019 19:16:05: 6000000 INFO @ Sat, 24 Aug 2019 19:16:08: 2000000 INFO @ Sat, 24 Aug 2019 19:16:12: 7000000 INFO @ Sat, 24 Aug 2019 19:16:16: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:16:20: 8000000 INFO @ Sat, 24 Aug 2019 19:16:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:16:22: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:16:22: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:16:24: 4000000 INFO @ Sat, 24 Aug 2019 19:16:29: 9000000 INFO @ Sat, 24 Aug 2019 19:16:29: 1000000 INFO @ Sat, 24 Aug 2019 19:16:32: 5000000 INFO @ Sat, 24 Aug 2019 19:16:36: 2000000 INFO @ Sat, 24 Aug 2019 19:16:37: 10000000 INFO @ Sat, 24 Aug 2019 19:16:41: 6000000 INFO @ Sat, 24 Aug 2019 19:16:43: 3000000 INFO @ Sat, 24 Aug 2019 19:16:45: 11000000 INFO @ Sat, 24 Aug 2019 19:16:50: 7000000 INFO @ Sat, 24 Aug 2019 19:16:50: 4000000 INFO @ Sat, 24 Aug 2019 19:16:53: 12000000 INFO @ Sat, 24 Aug 2019 19:16:57: 5000000 INFO @ Sat, 24 Aug 2019 19:16:58: 8000000 INFO @ Sat, 24 Aug 2019 19:17:00: 13000000 INFO @ Sat, 24 Aug 2019 19:17:04: 6000000 INFO @ Sat, 24 Aug 2019 19:17:06: 9000000 INFO @ Sat, 24 Aug 2019 19:17:08: 14000000 INFO @ Sat, 24 Aug 2019 19:17:11: 7000000 INFO @ Sat, 24 Aug 2019 19:17:13: 10000000 INFO @ Sat, 24 Aug 2019 19:17:16: #1 tag size is determined as 40 bps INFO @ Sat, 24 Aug 2019 19:17:16: #1 tag size = 40 INFO @ Sat, 24 Aug 2019 19:17:16: #1 total tags in treatment: 14970213 INFO @ Sat, 24 Aug 2019 19:17:16: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:17:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:17:16: #1 tags after filtering in treatment: 14970213 INFO @ Sat, 24 Aug 2019 19:17:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:17:16: #1 finished! INFO @ Sat, 24 Aug 2019 19:17:16: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:17:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:17:17: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:17:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:17:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:17:19: 8000000 INFO @ Sat, 24 Aug 2019 19:17:22: 11000000 INFO @ Sat, 24 Aug 2019 19:17:26: 9000000 INFO @ Sat, 24 Aug 2019 19:17:30: 12000000 INFO @ Sat, 24 Aug 2019 19:17:33: 10000000 INFO @ Sat, 24 Aug 2019 19:17:38: 13000000 INFO @ Sat, 24 Aug 2019 19:17:41: 11000000 INFO @ Sat, 24 Aug 2019 19:17:47: 14000000 INFO @ Sat, 24 Aug 2019 19:17:50: 12000000 INFO @ Sat, 24 Aug 2019 19:17:55: #1 tag size is determined as 40 bps INFO @ Sat, 24 Aug 2019 19:17:55: #1 tag size = 40 INFO @ Sat, 24 Aug 2019 19:17:55: #1 total tags in treatment: 14970213 INFO @ Sat, 24 Aug 2019 19:17:55: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:17:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:17:55: #1 tags after filtering in treatment: 14970213 INFO @ Sat, 24 Aug 2019 19:17:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:17:55: #1 finished! INFO @ Sat, 24 Aug 2019 19:17:55: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:17:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:17:56: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:17:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:17:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:17:57: 13000000 INFO @ Sat, 24 Aug 2019 19:18:06: 14000000 INFO @ Sat, 24 Aug 2019 19:18:13: #1 tag size is determined as 40 bps INFO @ Sat, 24 Aug 2019 19:18:13: #1 tag size = 40 INFO @ Sat, 24 Aug 2019 19:18:13: #1 total tags in treatment: 14970213 INFO @ Sat, 24 Aug 2019 19:18:13: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:18:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:18:14: #1 tags after filtering in treatment: 14970213 INFO @ Sat, 24 Aug 2019 19:18:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:18:14: #1 finished! INFO @ Sat, 24 Aug 2019 19:18:14: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:18:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:18:15: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:18:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:18:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2732433/ERX2732433.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。