Job ID = 11634008 sra ファイルのダウンロード中... Completed: 312207K bytes transferred in 6 seconds (391607K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 11399377 spots for /home/okishinya/chipatlas/results/sacCer3/ERX2558655/ERR2540237.sra Written 11399377 spots for /home/okishinya/chipatlas/results/sacCer3/ERX2558655/ERR2540237.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:27 11399377 reads; of these: 11399377 (100.00%) were unpaired; of these: 441382 (3.87%) aligned 0 times 8253419 (72.40%) aligned exactly 1 time 2704576 (23.73%) aligned >1 times 96.13% overall alignment rate Time searching: 00:02:27 Overall time: 00:02:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3883670 / 10957995 = 0.3544 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:25:36: # Command line: callpeak -t ERX2558655.bam -f BAM -g 12100000 -n ERX2558655.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX2558655.05 # format = BAM # ChIP-seq file = ['ERX2558655.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:25:36: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:25:36: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:25:36: # Command line: callpeak -t ERX2558655.bam -f BAM -g 12100000 -n ERX2558655.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX2558655.20 # format = BAM # ChIP-seq file = ['ERX2558655.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:25:36: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:25:36: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:25:36: # Command line: callpeak -t ERX2558655.bam -f BAM -g 12100000 -n ERX2558655.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX2558655.10 # format = BAM # ChIP-seq file = ['ERX2558655.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:25:36: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:25:36: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:25:42: 1000000 INFO @ Fri, 15 Feb 2019 09:25:42: 1000000 INFO @ Fri, 15 Feb 2019 09:25:42: 1000000 INFO @ Fri, 15 Feb 2019 09:25:48: 2000000 INFO @ Fri, 15 Feb 2019 09:25:49: 2000000 INFO @ Fri, 15 Feb 2019 09:25:49: 2000000 INFO @ Fri, 15 Feb 2019 09:25:55: 3000000 INFO @ Fri, 15 Feb 2019 09:25:55: 3000000 INFO @ Fri, 15 Feb 2019 09:25:55: 3000000 INFO @ Fri, 15 Feb 2019 09:26:01: 4000000 INFO @ Fri, 15 Feb 2019 09:26:02: 4000000 INFO @ Fri, 15 Feb 2019 09:26:02: 4000000 INFO @ Fri, 15 Feb 2019 09:26:07: 5000000 INFO @ Fri, 15 Feb 2019 09:26:09: 5000000 INFO @ Fri, 15 Feb 2019 09:26:09: 5000000 INFO @ Fri, 15 Feb 2019 09:26:13: 6000000 INFO @ Fri, 15 Feb 2019 09:26:15: 6000000 INFO @ Fri, 15 Feb 2019 09:26:16: 6000000 INFO @ Fri, 15 Feb 2019 09:26:20: 7000000 INFO @ Fri, 15 Feb 2019 09:26:20: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:26:20: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:26:20: #1 total tags in treatment: 7074325 INFO @ Fri, 15 Feb 2019 09:26:20: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:26:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:26:20: #1 tags after filtering in treatment: 7074325 INFO @ Fri, 15 Feb 2019 09:26:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:26:20: #1 finished! INFO @ Fri, 15 Feb 2019 09:26:20: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:26:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:26:21: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 09:26:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:26:21: Process for pairing-model is terminated! cat: ERX2558655.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558655.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558655.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558655.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:26:22: 7000000 INFO @ Fri, 15 Feb 2019 09:26:23: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:26:23: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:26:23: #1 total tags in treatment: 7074325 INFO @ Fri, 15 Feb 2019 09:26:23: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:26:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:26:23: 7000000 INFO @ Fri, 15 Feb 2019 09:26:23: #1 tags after filtering in treatment: 7074325 INFO @ Fri, 15 Feb 2019 09:26:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:26:23: #1 finished! INFO @ Fri, 15 Feb 2019 09:26:23: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:26:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:26:23: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:26:23: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:26:23: #1 total tags in treatment: 7074325 INFO @ Fri, 15 Feb 2019 09:26:23: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:26:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:26:23: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 09:26:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:26:23: Process for pairing-model is terminated! cat: ERX2558655.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Fri, 15 Feb 2019 09:26:23: #1 tags after filtering in treatment: 7074325 INFO @ Fri, 15 Feb 2019 09:26:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:26:23: #1 finished! INFO @ Fri, 15 Feb 2019 09:26:23: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:26:23: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558655.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558655.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558655.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:26:24: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 09:26:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:26:24: Process for pairing-model is terminated! cat: ERX2558655.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558655.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558655.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558655.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。