Job ID = 11634001 sra ファイルのダウンロード中... Completed: 85809K bytes transferred in 4 seconds (152880K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 3167900 spots for /home/okishinya/chipatlas/results/sacCer3/ERX2558648/ERR2540230.sra Written 3167900 spots for /home/okishinya/chipatlas/results/sacCer3/ERX2558648/ERR2540230.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:42 3167900 reads; of these: 3167900 (100.00%) were unpaired; of these: 64899 (2.05%) aligned 0 times 2539642 (80.17%) aligned exactly 1 time 563359 (17.78%) aligned >1 times 97.95% overall alignment rate Time searching: 00:00:43 Overall time: 00:00:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 552943 / 3103001 = 0.1782 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:20:58: # Command line: callpeak -t ERX2558648.bam -f BAM -g 12100000 -n ERX2558648.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX2558648.20 # format = BAM # ChIP-seq file = ['ERX2558648.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:20:58: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:20:58: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:20:58: # Command line: callpeak -t ERX2558648.bam -f BAM -g 12100000 -n ERX2558648.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX2558648.05 # format = BAM # ChIP-seq file = ['ERX2558648.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:20:58: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:20:58: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:20:58: # Command line: callpeak -t ERX2558648.bam -f BAM -g 12100000 -n ERX2558648.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX2558648.10 # format = BAM # ChIP-seq file = ['ERX2558648.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:20:58: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:20:58: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:21:05: 1000000 INFO @ Fri, 15 Feb 2019 09:21:05: 1000000 INFO @ Fri, 15 Feb 2019 09:21:05: 1000000 INFO @ Fri, 15 Feb 2019 09:21:11: 2000000 INFO @ Fri, 15 Feb 2019 09:21:12: 2000000 INFO @ Fri, 15 Feb 2019 09:21:12: 2000000 INFO @ Fri, 15 Feb 2019 09:21:15: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:21:15: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:21:15: #1 total tags in treatment: 2550058 INFO @ Fri, 15 Feb 2019 09:21:15: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:15: #1 tags after filtering in treatment: 2550058 INFO @ Fri, 15 Feb 2019 09:21:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:15: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:15: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:15: #2 number of paired peaks: 51 WARNING @ Fri, 15 Feb 2019 09:21:15: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:21:15: Process for pairing-model is terminated! cat: ERX2558648.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558648.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558648.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558648.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:21:16: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:21:16: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:21:16: #1 total tags in treatment: 2550058 INFO @ Fri, 15 Feb 2019 09:21:16: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:16: #1 tags after filtering in treatment: 2550058 INFO @ Fri, 15 Feb 2019 09:21:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:16: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:16: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:16: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:21:16: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:21:16: #1 total tags in treatment: 2550058 INFO @ Fri, 15 Feb 2019 09:21:16: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:16: #1 tags after filtering in treatment: 2550058 INFO @ Fri, 15 Feb 2019 09:21:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:16: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:16: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:16: #2 number of paired peaks: 51 WARNING @ Fri, 15 Feb 2019 09:21:16: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:21:16: Process for pairing-model is terminated! cat: ERX2558648.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558648.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558648.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558648.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません INFO @ Fri, 15 Feb 2019 09:21:16: #2 number of paired peaks: 51 WARNING @ Fri, 15 Feb 2019 09:21:16: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:21:16: Process for pairing-model is terminated! CompletedMACS2peakCalling cat: ERX2558648.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 7 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558648.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558648.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558648.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。