Job ID = 11633997 sra ファイルのダウンロード中... Completed: 262935K bytes transferred in 7 seconds (305967K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 9912948 spots for /home/okishinya/chipatlas/results/sacCer3/ERX2558644/ERR2540226.sra Written 9912948 spots for /home/okishinya/chipatlas/results/sacCer3/ERX2558644/ERR2540226.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:01 9912948 reads; of these: 9912948 (100.00%) were unpaired; of these: 207814 (2.10%) aligned 0 times 7649508 (77.17%) aligned exactly 1 time 2055626 (20.74%) aligned >1 times 97.90% overall alignment rate Time searching: 00:02:01 Overall time: 00:02:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2885500 / 9705134 = 0.2973 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:24:05: # Command line: callpeak -t ERX2558644.bam -f BAM -g 12100000 -n ERX2558644.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX2558644.10 # format = BAM # ChIP-seq file = ['ERX2558644.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:24:05: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:24:05: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:24:05: # Command line: callpeak -t ERX2558644.bam -f BAM -g 12100000 -n ERX2558644.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX2558644.05 # format = BAM # ChIP-seq file = ['ERX2558644.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:24:05: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:24:05: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:24:05: # Command line: callpeak -t ERX2558644.bam -f BAM -g 12100000 -n ERX2558644.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX2558644.20 # format = BAM # ChIP-seq file = ['ERX2558644.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:24:05: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:24:05: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:24:12: 1000000 INFO @ Fri, 15 Feb 2019 09:24:12: 1000000 INFO @ Fri, 15 Feb 2019 09:24:12: 1000000 INFO @ Fri, 15 Feb 2019 09:24:19: 2000000 INFO @ Fri, 15 Feb 2019 09:24:19: 2000000 INFO @ Fri, 15 Feb 2019 09:24:19: 2000000 INFO @ Fri, 15 Feb 2019 09:24:26: 3000000 INFO @ Fri, 15 Feb 2019 09:24:26: 3000000 INFO @ Fri, 15 Feb 2019 09:24:26: 3000000 INFO @ Fri, 15 Feb 2019 09:24:33: 4000000 INFO @ Fri, 15 Feb 2019 09:24:33: 4000000 INFO @ Fri, 15 Feb 2019 09:24:33: 4000000 INFO @ Fri, 15 Feb 2019 09:24:39: 5000000 INFO @ Fri, 15 Feb 2019 09:24:40: 5000000 INFO @ Fri, 15 Feb 2019 09:24:40: 5000000 INFO @ Fri, 15 Feb 2019 09:24:46: 6000000 INFO @ Fri, 15 Feb 2019 09:24:47: 6000000 INFO @ Fri, 15 Feb 2019 09:24:47: 6000000 INFO @ Fri, 15 Feb 2019 09:24:51: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:24:51: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:24:51: #1 total tags in treatment: 6819634 INFO @ Fri, 15 Feb 2019 09:24:51: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:24:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:24:51: #1 tags after filtering in treatment: 6819634 INFO @ Fri, 15 Feb 2019 09:24:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:24:51: #1 finished! INFO @ Fri, 15 Feb 2019 09:24:51: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:24:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:24:52: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 09:24:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:24:52: Process for pairing-model is terminated! cat: ERX2558644.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558644.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558644.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558644.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:24:52: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:24:52: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:24:52: #1 total tags in treatment: 6819634 INFO @ Fri, 15 Feb 2019 09:24:52: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:24:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:24:52: #1 tags after filtering in treatment: 6819634 INFO @ Fri, 15 Feb 2019 09:24:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:24:52: #1 finished! INFO @ Fri, 15 Feb 2019 09:24:52: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:24:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:24:52: #1 tag size is determined as 66 bps INFO @ Fri, 15 Feb 2019 09:24:52: #1 tag size = 66 INFO @ Fri, 15 Feb 2019 09:24:52: #1 total tags in treatment: 6819634 INFO @ Fri, 15 Feb 2019 09:24:52: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:24:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:24:53: #1 tags after filtering in treatment: 6819634 INFO @ Fri, 15 Feb 2019 09:24:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:24:53: #1 finished! INFO @ Fri, 15 Feb 2019 09:24:53: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:24:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:24:53: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 09:24:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:24:53: Process for pairing-model is terminated! cat: ERX2558644.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 7 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558644.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558644.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558644.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません INFO @ Fri, 15 Feb 2019 09:24:53: #2 number of paired peaks: 0 WARNING @ Fri, 15 Feb 2019 09:24:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:24:53: Process for pairing-model is terminated! CompletedMACS2peakCalling cat: ERX2558644.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX2558644.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558644.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX2558644.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。