Job ID = 2007623 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,478,800 reads read : 10,478,800 reads written : 10,478,800 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529736.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:01 10478800 reads; of these: 10478800 (100.00%) were unpaired; of these: 640518 (6.11%) aligned 0 times 7844772 (74.86%) aligned exactly 1 time 1993510 (19.02%) aligned >1 times 93.89% overall alignment rate Time searching: 00:07:01 Overall time: 00:07:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 9547792 / 9838282 = 0.9705 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 16:10:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:10:05: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:10:05: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:10:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:10:06: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:10:06: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:10:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:10:07: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:10:07: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:10:09: #1 tag size is determined as 76 bps INFO @ Fri, 05 Jul 2019 16:10:09: #1 tag size = 76 INFO @ Fri, 05 Jul 2019 16:10:09: #1 total tags in treatment: 290490 INFO @ Fri, 05 Jul 2019 16:10:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:10:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:10:09: #1 tags after filtering in treatment: 290490 INFO @ Fri, 05 Jul 2019 16:10:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:10:09: #1 finished! INFO @ Fri, 05 Jul 2019 16:10:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:10:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:10:09: #2 number of paired peaks: 530 WARNING @ Fri, 05 Jul 2019 16:10:09: Fewer paired peaks (530) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 530 pairs to build model! INFO @ Fri, 05 Jul 2019 16:10:09: start model_add_line... INFO @ Fri, 05 Jul 2019 16:10:09: start X-correlation... INFO @ Fri, 05 Jul 2019 16:10:09: end of X-cor INFO @ Fri, 05 Jul 2019 16:10:09: #2 finished! INFO @ Fri, 05 Jul 2019 16:10:09: #2 predicted fragment length is 122 bps INFO @ Fri, 05 Jul 2019 16:10:09: #2 alternative fragment length(s) may be 122,569 bps INFO @ Fri, 05 Jul 2019 16:10:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.05_model.r WARNING @ Fri, 05 Jul 2019 16:10:09: #2 Since the d (122) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 16:10:09: #2 You may need to consider one of the other alternative d(s): 122,569 WARNING @ Fri, 05 Jul 2019 16:10:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 16:10:09: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:10:09: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:10:09: #1 tag size is determined as 76 bps INFO @ Fri, 05 Jul 2019 16:10:09: #1 tag size = 76 INFO @ Fri, 05 Jul 2019 16:10:09: #1 total tags in treatment: 290490 INFO @ Fri, 05 Jul 2019 16:10:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:10:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:10:09: #1 tags after filtering in treatment: 290490 INFO @ Fri, 05 Jul 2019 16:10:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:10:09: #1 finished! INFO @ Fri, 05 Jul 2019 16:10:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:10:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:10:09: #2 number of paired peaks: 530 WARNING @ Fri, 05 Jul 2019 16:10:09: Fewer paired peaks (530) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 530 pairs to build model! INFO @ Fri, 05 Jul 2019 16:10:09: start model_add_line... INFO @ Fri, 05 Jul 2019 16:10:09: start X-correlation... INFO @ Fri, 05 Jul 2019 16:10:09: end of X-cor INFO @ Fri, 05 Jul 2019 16:10:09: #2 finished! INFO @ Fri, 05 Jul 2019 16:10:09: #2 predicted fragment length is 122 bps INFO @ Fri, 05 Jul 2019 16:10:09: #2 alternative fragment length(s) may be 122,569 bps INFO @ Fri, 05 Jul 2019 16:10:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.10_model.r WARNING @ Fri, 05 Jul 2019 16:10:09: #2 Since the d (122) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 16:10:09: #2 You may need to consider one of the other alternative d(s): 122,569 WARNING @ Fri, 05 Jul 2019 16:10:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 16:10:09: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:10:09: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:10:10: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:10:10: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.05_peaks.xls INFO @ Fri, 05 Jul 2019 16:10:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:10:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.05_summits.bed INFO @ Fri, 05 Jul 2019 16:10:10: Done! INFO @ Fri, 05 Jul 2019 16:10:11: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:10:11: #1 tag size is determined as 76 bps INFO @ Fri, 05 Jul 2019 16:10:11: #1 tag size = 76 INFO @ Fri, 05 Jul 2019 16:10:11: #1 total tags in treatment: 290490 INFO @ Fri, 05 Jul 2019 16:10:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:10:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:10:11: #1 tags after filtering in treatment: 290490 INFO @ Fri, 05 Jul 2019 16:10:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:10:11: #1 finished! INFO @ Fri, 05 Jul 2019 16:10:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:10:11: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (575 records, 4 fields): 3 millis INFO @ Fri, 05 Jul 2019 16:10:11: #2 number of paired peaks: 530 WARNING @ Fri, 05 Jul 2019 16:10:11: Fewer paired peaks (530) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 530 pairs to build model! INFO @ Fri, 05 Jul 2019 16:10:11: start model_add_line... INFO @ Fri, 05 Jul 2019 16:10:11: start X-correlation... INFO @ Fri, 05 Jul 2019 16:10:11: end of X-cor INFO @ Fri, 05 Jul 2019 16:10:11: #2 finished! INFO @ Fri, 05 Jul 2019 16:10:11: #2 predicted fragment length is 122 bps INFO @ Fri, 05 Jul 2019 16:10:11: #2 alternative fragment length(s) may be 122,569 bps INFO @ Fri, 05 Jul 2019 16:10:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.20_model.r WARNING @ Fri, 05 Jul 2019 16:10:11: #2 Since the d (122) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 16:10:11: #2 You may need to consider one of the other alternative d(s): 122,569 WARNING @ Fri, 05 Jul 2019 16:10:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 16:10:11: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:10:11: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:10:11: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.10_peaks.xls INFO @ Fri, 05 Jul 2019 16:10:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:10:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.10_summits.bed INFO @ Fri, 05 Jul 2019 16:10:11: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (295 records, 4 fields): 3 millis BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 16:10:12: #3 Call peaks for each chromosome... CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:10:12: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.20_peaks.xls INFO @ Fri, 05 Jul 2019 16:10:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:10:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548225/ERX2548225.20_summits.bed INFO @ Fri, 05 Jul 2019 16:10:12: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (145 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。 CompletedMACS2peakCalling