Job ID = 2006925 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,850,627 reads read : 2,850,627 reads written : 2,850,627 spots read : 2,768,214 reads read : 2,768,214 reads written : 2,768,214 spots read : 2,859,269 reads read : 2,859,269 reads written : 2,859,269 spots read : 2,661,650 reads read : 2,661,650 reads written : 2,661,650 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529589.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529590.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:40 11139760 reads; of these: 11139760 (100.00%) were unpaired; of these: 668009 (6.00%) aligned 0 times 8791097 (78.92%) aligned exactly 1 time 1680654 (15.09%) aligned >1 times 94.00% overall alignment rate Time searching: 00:03:40 Overall time: 00:03:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10258863 / 10471751 = 0.9797 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 15:57:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:57:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:57:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:57:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:57:31: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:57:31: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:57:32: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:57:32: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:57:32: #1 total tags in treatment: 212888 INFO @ Fri, 05 Jul 2019 15:57:32: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:57:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:57:32: #1 tags after filtering in treatment: 212888 INFO @ Fri, 05 Jul 2019 15:57:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:57:32: #1 finished! INFO @ Fri, 05 Jul 2019 15:57:32: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:57:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:57:32: #2 number of paired peaks: 487 WARNING @ Fri, 05 Jul 2019 15:57:32: Fewer paired peaks (487) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 487 pairs to build model! INFO @ Fri, 05 Jul 2019 15:57:32: start model_add_line... INFO @ Fri, 05 Jul 2019 15:57:32: start X-correlation... INFO @ Fri, 05 Jul 2019 15:57:32: end of X-cor INFO @ Fri, 05 Jul 2019 15:57:32: #2 finished! INFO @ Fri, 05 Jul 2019 15:57:32: #2 predicted fragment length is 68 bps INFO @ Fri, 05 Jul 2019 15:57:32: #2 alternative fragment length(s) may be 68,118,240,250,269,392,447,470,494,529,541,592 bps INFO @ Fri, 05 Jul 2019 15:57:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.05_model.r WARNING @ Fri, 05 Jul 2019 15:57:32: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:57:32: #2 You may need to consider one of the other alternative d(s): 68,118,240,250,269,392,447,470,494,529,541,592 WARNING @ Fri, 05 Jul 2019 15:57:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:57:32: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:57:32: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 15:57:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:57:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:57:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:57:33: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:57:33: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:57:33: #1 total tags in treatment: 212888 INFO @ Fri, 05 Jul 2019 15:57:33: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:57:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:57:33: #1 tags after filtering in treatment: 212888 INFO @ Fri, 05 Jul 2019 15:57:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:57:33: #1 finished! INFO @ Fri, 05 Jul 2019 15:57:33: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:57:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:57:33: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 15:57:33: #2 number of paired peaks: 487 WARNING @ Fri, 05 Jul 2019 15:57:33: Fewer paired peaks (487) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 487 pairs to build model! INFO @ Fri, 05 Jul 2019 15:57:33: start model_add_line... INFO @ Fri, 05 Jul 2019 15:57:33: start X-correlation... INFO @ Fri, 05 Jul 2019 15:57:33: end of X-cor INFO @ Fri, 05 Jul 2019 15:57:33: #2 finished! INFO @ Fri, 05 Jul 2019 15:57:33: #2 predicted fragment length is 68 bps INFO @ Fri, 05 Jul 2019 15:57:33: #2 alternative fragment length(s) may be 68,118,240,250,269,392,447,470,494,529,541,592 bps INFO @ Fri, 05 Jul 2019 15:57:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.10_model.r WARNING @ Fri, 05 Jul 2019 15:57:33: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:57:33: #2 You may need to consider one of the other alternative d(s): 68,118,240,250,269,392,447,470,494,529,541,592 WARNING @ Fri, 05 Jul 2019 15:57:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:57:33: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:57:33: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 15:57:33: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.05_peaks.xls INFO @ Fri, 05 Jul 2019 15:57:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:57:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.05_summits.bed INFO @ Fri, 05 Jul 2019 15:57:33: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (78 records, 4 fields): 2 millis INFO @ Fri, 05 Jul 2019 15:57:34: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 15:57:34: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.10_peaks.xls INFO @ Fri, 05 Jul 2019 15:57:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:57:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.10_summits.bed INFO @ Fri, 05 Jul 2019 15:57:34: Done! INFO @ Fri, 05 Jul 2019 15:57:34: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:57:34: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:57:34: #1 total tags in treatment: 212888 INFO @ Fri, 05 Jul 2019 15:57:34: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:57:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:57:34: #1 tags after filtering in treatment: 212888 INFO @ Fri, 05 Jul 2019 15:57:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:57:34: #1 finished! INFO @ Fri, 05 Jul 2019 15:57:34: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:57:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:57:34: #2 number of paired peaks: 487 WARNING @ Fri, 05 Jul 2019 15:57:34: Fewer paired peaks (487) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 487 pairs to build model! INFO @ Fri, 05 Jul 2019 15:57:34: start model_add_line... INFO @ Fri, 05 Jul 2019 15:57:34: start X-correlation... INFO @ Fri, 05 Jul 2019 15:57:34: end of X-cor INFO @ Fri, 05 Jul 2019 15:57:34: #2 finished! INFO @ Fri, 05 Jul 2019 15:57:34: #2 predicted fragment length is 68 bps INFO @ Fri, 05 Jul 2019 15:57:34: #2 alternative fragment length(s) may be 68,118,240,250,269,392,447,470,494,529,541,592 bps INFO @ Fri, 05 Jul 2019 15:57:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.20_model.r WARNING @ Fri, 05 Jul 2019 15:57:34: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:57:34: #2 You may need to consider one of the other alternative d(s): 68,118,240,250,269,392,447,470,494,529,541,592 WARNING @ Fri, 05 Jul 2019 15:57:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:57:34: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:57:34: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (21 records, 4 fields): 1 millis INFO @ Fri, 05 Jul 2019 15:57:35: #3 Call peaks for each chromosome... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 15:57:35: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.20_peaks.xls INFO @ Fri, 05 Jul 2019 15:57:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:57:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548182/ERX2548182.20_summits.bed INFO @ Fri, 05 Jul 2019 15:57:35: Done! pass1 - making usageList (2 chroms): 1 millis pass2 - checking and writing primary data (2 records, 4 fields): 1 millis BigWig に変換しました。 CompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling