Job ID = 2006329 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 1,260,289 reads read : 1,260,289 reads written : 1,260,289 spots read : 1,212,036 reads read : 1,212,036 reads written : 1,212,036 spots read : 1,241,712 reads read : 1,241,712 reads written : 1,241,712 spots read : 1,257,143 reads read : 1,257,143 reads written : 1,257,143 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529506.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529507.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529509.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:47 4971180 reads; of these: 4971180 (100.00%) were unpaired; of these: 235945 (4.75%) aligned 0 times 4000868 (80.48%) aligned exactly 1 time 734367 (14.77%) aligned >1 times 95.25% overall alignment rate Time searching: 00:02:47 Overall time: 00:02:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 4681150 / 4735235 = 0.9886 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 15:37:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:37:59: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:37:59: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:37:59: #1 tag size is determined as 126 bps INFO @ Fri, 05 Jul 2019 15:37:59: #1 tag size = 126 INFO @ Fri, 05 Jul 2019 15:37:59: #1 total tags in treatment: 54085 INFO @ Fri, 05 Jul 2019 15:37:59: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:37:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:37:59: #1 tags after filtering in treatment: 54085 INFO @ Fri, 05 Jul 2019 15:37:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:37:59: #1 finished! INFO @ Fri, 05 Jul 2019 15:37:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:37:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:37:59: #2 number of paired peaks: 320 WARNING @ Fri, 05 Jul 2019 15:37:59: Fewer paired peaks (320) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 320 pairs to build model! INFO @ Fri, 05 Jul 2019 15:37:59: start model_add_line... INFO @ Fri, 05 Jul 2019 15:37:59: start X-correlation... INFO @ Fri, 05 Jul 2019 15:37:59: end of X-cor INFO @ Fri, 05 Jul 2019 15:37:59: #2 finished! INFO @ Fri, 05 Jul 2019 15:37:59: #2 predicted fragment length is 158 bps INFO @ Fri, 05 Jul 2019 15:37:59: #2 alternative fragment length(s) may be 43,83,112,137,158,175,194,240,372,447,506,554,582 bps INFO @ Fri, 05 Jul 2019 15:37:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.05_model.r WARNING @ Fri, 05 Jul 2019 15:37:59: #2 Since the d (158) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:37:59: #2 You may need to consider one of the other alternative d(s): 43,83,112,137,158,175,194,240,372,447,506,554,582 WARNING @ Fri, 05 Jul 2019 15:37:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:37:59: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:37:59: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 15:38:00: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 15:38:00: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.05_peaks.xls INFO @ Fri, 05 Jul 2019 15:38:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:38:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.05_summits.bed INFO @ Fri, 05 Jul 2019 15:38:00: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (13 records, 4 fields): 2 millis BigWig に変換しました。 INFO @ Fri, 05 Jul 2019 15:38:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:38:00: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:38:00: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:38:00: #1 tag size is determined as 126 bps INFO @ Fri, 05 Jul 2019 15:38:00: #1 tag size = 126 INFO @ Fri, 05 Jul 2019 15:38:00: #1 total tags in treatment: 54085 INFO @ Fri, 05 Jul 2019 15:38:00: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:38:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:38:00: #1 tags after filtering in treatment: 54085 INFO @ Fri, 05 Jul 2019 15:38:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:38:00: #1 finished! INFO @ Fri, 05 Jul 2019 15:38:00: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:38:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:38:00: #2 number of paired peaks: 320 WARNING @ Fri, 05 Jul 2019 15:38:00: Fewer paired peaks (320) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 320 pairs to build model! INFO @ Fri, 05 Jul 2019 15:38:00: start model_add_line... INFO @ Fri, 05 Jul 2019 15:38:00: start X-correlation... INFO @ Fri, 05 Jul 2019 15:38:00: end of X-cor INFO @ Fri, 05 Jul 2019 15:38:00: #2 finished! INFO @ Fri, 05 Jul 2019 15:38:00: #2 predicted fragment length is 158 bps INFO @ Fri, 05 Jul 2019 15:38:00: #2 alternative fragment length(s) may be 43,83,112,137,158,175,194,240,372,447,506,554,582 bps INFO @ Fri, 05 Jul 2019 15:38:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.10_model.r WARNING @ Fri, 05 Jul 2019 15:38:00: #2 Since the d (158) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:38:00: #2 You may need to consider one of the other alternative d(s): 43,83,112,137,158,175,194,240,372,447,506,554,582 WARNING @ Fri, 05 Jul 2019 15:38:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:38:00: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:38:00: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 15:38:01: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 15:38:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:38:01: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:38:01: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:38:01: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.10_peaks.xls INFO @ Fri, 05 Jul 2019 15:38:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:38:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.10_summits.bed INFO @ Fri, 05 Jul 2019 15:38:01: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (12 records, 4 fields): 2 millis INFO @ Fri, 05 Jul 2019 15:38:01: #1 tag size is determined as 126 bps INFO @ Fri, 05 Jul 2019 15:38:01: #1 tag size = 126 INFO @ Fri, 05 Jul 2019 15:38:01: #1 total tags in treatment: 54085 INFO @ Fri, 05 Jul 2019 15:38:01: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:38:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:38:01: #1 tags after filtering in treatment: 54085 INFO @ Fri, 05 Jul 2019 15:38:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:38:01: #1 finished! INFO @ Fri, 05 Jul 2019 15:38:01: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:38:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:38:01: #2 number of paired peaks: 320 WARNING @ Fri, 05 Jul 2019 15:38:01: Fewer paired peaks (320) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 320 pairs to build model! INFO @ Fri, 05 Jul 2019 15:38:01: start model_add_line... INFO @ Fri, 05 Jul 2019 15:38:01: start X-correlation... INFO @ Fri, 05 Jul 2019 15:38:01: end of X-cor INFO @ Fri, 05 Jul 2019 15:38:01: #2 finished! INFO @ Fri, 05 Jul 2019 15:38:01: #2 predicted fragment length is 158 bps INFO @ Fri, 05 Jul 2019 15:38:01: #2 alternative fragment length(s) may be 43,83,112,137,158,175,194,240,372,447,506,554,582 bps INFO @ Fri, 05 Jul 2019 15:38:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.20_model.r WARNING @ Fri, 05 Jul 2019 15:38:01: #2 Since the d (158) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:38:01: #2 You may need to consider one of the other alternative d(s): 43,83,112,137,158,175,194,240,372,447,506,554,582 WARNING @ Fri, 05 Jul 2019 15:38:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:38:01: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:38:01: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 15:38:01: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 15:38:02: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.20_peaks.xls INFO @ Fri, 05 Jul 2019 15:38:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:38:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548160/ERX2548160.20_summits.bed INFO @ Fri, 05 Jul 2019 15:38:02: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (6 records, 4 fields): 1 millis CompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling