Job ID = 2006278 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 1,788,430 reads read : 1,788,430 reads written : 1,788,430 spots read : 1,699,407 reads read : 1,699,407 reads written : 1,699,407 spots read : 1,735,451 reads read : 1,735,451 reads written : 1,735,451 spots read : 1,713,953 reads read : 1,713,953 reads written : 1,713,953 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529406.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529407.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529408.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529409.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:14 6937241 reads; of these: 6937241 (100.00%) were unpaired; of these: 318692 (4.59%) aligned 0 times 5554439 (80.07%) aligned exactly 1 time 1064110 (15.34%) aligned >1 times 95.41% overall alignment rate Time searching: 00:03:14 Overall time: 00:03:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5785749 / 6618549 = 0.8742 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 15:22:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:22:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:22:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:22:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:22:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:22:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:22:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:22:35: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:22:35: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:22:41: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:22:41: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:22:41: #1 total tags in treatment: 832800 INFO @ Fri, 05 Jul 2019 15:22:41: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:22:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:22:41: #1 tags after filtering in treatment: 832800 INFO @ Fri, 05 Jul 2019 15:22:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:22:41: #1 finished! INFO @ Fri, 05 Jul 2019 15:22:41: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:22:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:22:41: #2 number of paired peaks: 61 WARNING @ Fri, 05 Jul 2019 15:22:41: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 15:22:41: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 15:22:42: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:22:42: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:22:42: #1 total tags in treatment: 832800 INFO @ Fri, 05 Jul 2019 15:22:42: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:22:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:22:42: #1 tags after filtering in treatment: 832800 INFO @ Fri, 05 Jul 2019 15:22:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:22:42: #1 finished! INFO @ Fri, 05 Jul 2019 15:22:42: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:22:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:22:42: #2 number of paired peaks: 61 WARNING @ Fri, 05 Jul 2019 15:22:42: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 15:22:42: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 15:22:44: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:22:44: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:22:44: #1 total tags in treatment: 832800 INFO @ Fri, 05 Jul 2019 15:22:44: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:22:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:22:44: #1 tags after filtering in treatment: 832800 INFO @ Fri, 05 Jul 2019 15:22:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:22:44: #1 finished! INFO @ Fri, 05 Jul 2019 15:22:44: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:22:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:22:44: #2 number of paired peaks: 61 WARNING @ Fri, 05 Jul 2019 15:22:44: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 15:22:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.10_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX2548129/ERX2548129.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。