Job ID = 2006009 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,677,845 reads read : 2,677,845 reads written : 2,677,845 spots read : 2,580,124 reads read : 2,580,124 reads written : 2,580,124 spots read : 2,609,626 reads read : 2,609,626 reads written : 2,609,626 spots read : 2,567,405 reads read : 2,567,405 reads written : 2,567,405 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529282.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529283.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529284.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR2529285.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:34 10435000 reads; of these: 10435000 (100.00%) were unpaired; of these: 387015 (3.71%) aligned 0 times 8320251 (79.73%) aligned exactly 1 time 1727734 (16.56%) aligned >1 times 96.29% overall alignment rate Time searching: 00:04:34 Overall time: 00:04:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 9524086 / 10047985 = 0.9479 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 15:00:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:00:53: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:00:53: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:00:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:00:54: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:00:54: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:00:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 15:00:55: #1 read tag files... INFO @ Fri, 05 Jul 2019 15:00:55: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 15:00:57: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:00:57: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:00:57: #1 total tags in treatment: 523899 INFO @ Fri, 05 Jul 2019 15:00:57: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:00:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:00:57: #1 tags after filtering in treatment: 523899 INFO @ Fri, 05 Jul 2019 15:00:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:00:57: #1 finished! INFO @ Fri, 05 Jul 2019 15:00:57: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:00:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:00:57: #2 number of paired peaks: 525 WARNING @ Fri, 05 Jul 2019 15:00:57: Fewer paired peaks (525) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 525 pairs to build model! INFO @ Fri, 05 Jul 2019 15:00:57: start model_add_line... INFO @ Fri, 05 Jul 2019 15:00:57: start X-correlation... INFO @ Fri, 05 Jul 2019 15:00:57: end of X-cor INFO @ Fri, 05 Jul 2019 15:00:57: #2 finished! INFO @ Fri, 05 Jul 2019 15:00:57: #2 predicted fragment length is 68 bps INFO @ Fri, 05 Jul 2019 15:00:57: #2 alternative fragment length(s) may be 68 bps INFO @ Fri, 05 Jul 2019 15:00:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.05_model.r WARNING @ Fri, 05 Jul 2019 15:00:57: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:00:57: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Fri, 05 Jul 2019 15:00:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:00:57: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:00:57: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 15:00:58: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:00:58: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:00:58: #1 total tags in treatment: 523899 INFO @ Fri, 05 Jul 2019 15:00:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:00:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:00:59: #1 tags after filtering in treatment: 523899 INFO @ Fri, 05 Jul 2019 15:00:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:00:59: #1 finished! INFO @ Fri, 05 Jul 2019 15:00:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:00:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:00:59: #2 number of paired peaks: 525 WARNING @ Fri, 05 Jul 2019 15:00:59: Fewer paired peaks (525) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 525 pairs to build model! INFO @ Fri, 05 Jul 2019 15:00:59: start model_add_line... INFO @ Fri, 05 Jul 2019 15:00:59: start X-correlation... INFO @ Fri, 05 Jul 2019 15:00:59: end of X-cor INFO @ Fri, 05 Jul 2019 15:00:59: #2 finished! INFO @ Fri, 05 Jul 2019 15:00:59: #2 predicted fragment length is 68 bps INFO @ Fri, 05 Jul 2019 15:00:59: #2 alternative fragment length(s) may be 68 bps INFO @ Fri, 05 Jul 2019 15:00:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.10_model.r WARNING @ Fri, 05 Jul 2019 15:00:59: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:00:59: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Fri, 05 Jul 2019 15:00:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:00:59: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:00:59: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 15:00:59: #1 tag size is determined as 75 bps INFO @ Fri, 05 Jul 2019 15:00:59: #1 tag size = 75 INFO @ Fri, 05 Jul 2019 15:00:59: #1 total tags in treatment: 523899 INFO @ Fri, 05 Jul 2019 15:00:59: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 15:00:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 15:00:59: #1 tags after filtering in treatment: 523899 INFO @ Fri, 05 Jul 2019 15:00:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 15:00:59: #1 finished! INFO @ Fri, 05 Jul 2019 15:00:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 15:00:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 15:00:59: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 15:00:59: #2 number of paired peaks: 525 WARNING @ Fri, 05 Jul 2019 15:00:59: Fewer paired peaks (525) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 525 pairs to build model! INFO @ Fri, 05 Jul 2019 15:00:59: start model_add_line... INFO @ Fri, 05 Jul 2019 15:00:59: start X-correlation... INFO @ Fri, 05 Jul 2019 15:00:59: end of X-cor INFO @ Fri, 05 Jul 2019 15:00:59: #2 finished! INFO @ Fri, 05 Jul 2019 15:00:59: #2 predicted fragment length is 68 bps INFO @ Fri, 05 Jul 2019 15:00:59: #2 alternative fragment length(s) may be 68 bps INFO @ Fri, 05 Jul 2019 15:00:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.20_model.r WARNING @ Fri, 05 Jul 2019 15:00:59: #2 Since the d (68) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 05 Jul 2019 15:00:59: #2 You may need to consider one of the other alternative d(s): 68 WARNING @ Fri, 05 Jul 2019 15:00:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 05 Jul 2019 15:00:59: #3 Call peaks... INFO @ Fri, 05 Jul 2019 15:00:59: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 15:01:00: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.05_peaks.xls INFO @ Fri, 05 Jul 2019 15:01:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:01:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.05_summits.bed INFO @ Fri, 05 Jul 2019 15:01:00: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (243 records, 4 fields): 3 millis INFO @ Fri, 05 Jul 2019 15:01:01: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 15:01:01: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.10_peaks.xls INFO @ Fri, 05 Jul 2019 15:01:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:01:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.10_summits.bed INFO @ Fri, 05 Jul 2019 15:01:01: Done! INFO @ Fri, 05 Jul 2019 15:01:01: #3 Call peaks for each chromosome... pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (167 records, 4 fields): 3 millis INFO @ Fri, 05 Jul 2019 15:01:02: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.20_peaks.xls INFO @ Fri, 05 Jul 2019 15:01:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 15:01:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX2548092/ERX2548092.20_summits.bed INFO @ Fri, 05 Jul 2019 15:01:02: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (139 records, 4 fields): 3 millis CompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。