Job ID = 11633964 sra ファイルのダウンロード中... Completed: 676920K bytes transferred in 8 seconds (657375K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 21724698 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1424551/ERR1353076.sra Written 21724698 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1424551/ERR1353076.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:42 21724698 reads; of these: 21724698 (100.00%) were unpaired; of these: 2534327 (11.67%) aligned 0 times 15703342 (72.28%) aligned exactly 1 time 3487029 (16.05%) aligned >1 times 88.33% overall alignment rate Time searching: 00:03:42 Overall time: 00:03:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 16860125 / 19190371 = 0.8786 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:26:35: # Command line: callpeak -t ERX1424551.bam -f BAM -g 12100000 -n ERX1424551.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1424551.10 # format = BAM # ChIP-seq file = ['ERX1424551.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:26:35: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:26:35: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:26:35: # Command line: callpeak -t ERX1424551.bam -f BAM -g 12100000 -n ERX1424551.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1424551.05 # format = BAM # ChIP-seq file = ['ERX1424551.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:26:35: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:26:35: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:26:35: # Command line: callpeak -t ERX1424551.bam -f BAM -g 12100000 -n ERX1424551.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1424551.20 # format = BAM # ChIP-seq file = ['ERX1424551.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:26:35: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:26:35: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:26:41: 1000000 INFO @ Fri, 15 Feb 2019 09:26:41: 1000000 INFO @ Fri, 15 Feb 2019 09:26:41: 1000000 INFO @ Fri, 15 Feb 2019 09:26:48: 2000000 INFO @ Fri, 15 Feb 2019 09:26:48: 2000000 INFO @ Fri, 15 Feb 2019 09:26:48: 2000000 INFO @ Fri, 15 Feb 2019 09:26:50: #1 tag size is determined as 48 bps INFO @ Fri, 15 Feb 2019 09:26:50: #1 tag size = 48 INFO @ Fri, 15 Feb 2019 09:26:50: #1 total tags in treatment: 2330246 INFO @ Fri, 15 Feb 2019 09:26:50: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:26:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:26:50: #1 tags after filtering in treatment: 2330246 INFO @ Fri, 15 Feb 2019 09:26:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:26:50: #1 finished! INFO @ Fri, 15 Feb 2019 09:26:50: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:26:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:26:50: #1 tag size is determined as 48 bps INFO @ Fri, 15 Feb 2019 09:26:50: #1 tag size = 48 INFO @ Fri, 15 Feb 2019 09:26:50: #1 total tags in treatment: 2330246 INFO @ Fri, 15 Feb 2019 09:26:50: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:26:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:26:50: #1 tags after filtering in treatment: 2330246 INFO @ Fri, 15 Feb 2019 09:26:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:26:50: #1 finished! INFO @ Fri, 15 Feb 2019 09:26:50: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:26:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:26:50: #1 tag size is determined as 48 bps INFO @ Fri, 15 Feb 2019 09:26:50: #1 tag size = 48 INFO @ Fri, 15 Feb 2019 09:26:50: #1 total tags in treatment: 2330246 INFO @ Fri, 15 Feb 2019 09:26:50: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:26:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:26:50: #2 number of paired peaks: 534 WARNING @ Fri, 15 Feb 2019 09:26:50: Fewer paired peaks (534) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 534 pairs to build model! INFO @ Fri, 15 Feb 2019 09:26:50: start model_add_line... INFO @ Fri, 15 Feb 2019 09:26:50: #1 tags after filtering in treatment: 2330246 INFO @ Fri, 15 Feb 2019 09:26:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:26:50: #1 finished! INFO @ Fri, 15 Feb 2019 09:26:50: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:26:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:26:50: start X-correlation... INFO @ Fri, 15 Feb 2019 09:26:50: end of X-cor INFO @ Fri, 15 Feb 2019 09:26:50: #2 finished! INFO @ Fri, 15 Feb 2019 09:26:50: #2 predicted fragment length is 175 bps INFO @ Fri, 15 Feb 2019 09:26:50: #2 alternative fragment length(s) may be 175 bps INFO @ Fri, 15 Feb 2019 09:26:50: #2.2 Generate R script for model : ERX1424551.05_model.r INFO @ Fri, 15 Feb 2019 09:26:50: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:26:50: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:26:50: #2 number of paired peaks: 534 WARNING @ Fri, 15 Feb 2019 09:26:50: Fewer paired peaks (534) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 534 pairs to build model! INFO @ Fri, 15 Feb 2019 09:26:50: start model_add_line... INFO @ Fri, 15 Feb 2019 09:26:50: start X-correlation... INFO @ Fri, 15 Feb 2019 09:26:50: end of X-cor INFO @ Fri, 15 Feb 2019 09:26:50: #2 finished! INFO @ Fri, 15 Feb 2019 09:26:50: #2 predicted fragment length is 175 bps INFO @ Fri, 15 Feb 2019 09:26:50: #2 alternative fragment length(s) may be 175 bps INFO @ Fri, 15 Feb 2019 09:26:50: #2.2 Generate R script for model : ERX1424551.20_model.r INFO @ Fri, 15 Feb 2019 09:26:50: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:26:50: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:26:51: #2 number of paired peaks: 534 WARNING @ Fri, 15 Feb 2019 09:26:51: Fewer paired peaks (534) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 534 pairs to build model! INFO @ Fri, 15 Feb 2019 09:26:51: start model_add_line... INFO @ Fri, 15 Feb 2019 09:26:51: start X-correlation... INFO @ Fri, 15 Feb 2019 09:26:51: end of X-cor INFO @ Fri, 15 Feb 2019 09:26:51: #2 finished! INFO @ Fri, 15 Feb 2019 09:26:51: #2 predicted fragment length is 175 bps INFO @ Fri, 15 Feb 2019 09:26:51: #2 alternative fragment length(s) may be 175 bps INFO @ Fri, 15 Feb 2019 09:26:51: #2.2 Generate R script for model : ERX1424551.10_model.r INFO @ Fri, 15 Feb 2019 09:26:51: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:26:51: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:27:00: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:27:00: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:27:01: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write output xls file... ERX1424551.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write peak in narrowPeak format file... ERX1424551.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write summits bed file... ERX1424551.20_summits.bed INFO @ Fri, 15 Feb 2019 09:27:03: Done! pass1 - making usageList (16 chroms): 6 millis pass2 - checking and writing primary data (2287 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write output xls file... ERX1424551.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write peak in narrowPeak format file... ERX1424551.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write summits bed file... ERX1424551.05_summits.bed INFO @ Fri, 15 Feb 2019 09:27:03: Done! pass1 - making usageList (16 chroms): 7 millis pass2 - checking and writing primary data (3376 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write output xls file... ERX1424551.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write peak in narrowPeak format file... ERX1424551.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:27:03: #4 Write summits bed file... ERX1424551.10_summits.bed INFO @ Fri, 15 Feb 2019 09:27:04: Done! pass1 - making usageList (16 chroms): 9 millis pass2 - checking and writing primary data (2913 records, 4 fields): 15 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。