Job ID = 11633959 sra ファイルのダウンロード中... Completed: 661582K bytes transferred in 9 seconds (586755K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 20903253 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1424546/ERR1353071.sra Written 20903253 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1424546/ERR1353071.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:27 20903253 reads; of these: 20903253 (100.00%) were unpaired; of these: 13808633 (66.06%) aligned 0 times 4995854 (23.90%) aligned exactly 1 time 2098766 (10.04%) aligned >1 times 33.94% overall alignment rate Time searching: 00:02:27 Overall time: 00:02:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5547220 / 7094620 = 0.7819 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:23:01: # Command line: callpeak -t ERX1424546.bam -f BAM -g 12100000 -n ERX1424546.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1424546.10 # format = BAM # ChIP-seq file = ['ERX1424546.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:23:01: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:23:01: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:23:01: # Command line: callpeak -t ERX1424546.bam -f BAM -g 12100000 -n ERX1424546.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1424546.20 # format = BAM # ChIP-seq file = ['ERX1424546.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:23:01: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:23:01: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:23:01: # Command line: callpeak -t ERX1424546.bam -f BAM -g 12100000 -n ERX1424546.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1424546.05 # format = BAM # ChIP-seq file = ['ERX1424546.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:23:01: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:23:01: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:23:07: 1000000 INFO @ Fri, 15 Feb 2019 09:23:07: 1000000 INFO @ Fri, 15 Feb 2019 09:23:07: 1000000 INFO @ Fri, 15 Feb 2019 09:23:10: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:23:10: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:23:10: #1 total tags in treatment: 1547400 INFO @ Fri, 15 Feb 2019 09:23:10: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:23:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:23:10: #1 tags after filtering in treatment: 1547400 INFO @ Fri, 15 Feb 2019 09:23:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:23:10: #1 finished! INFO @ Fri, 15 Feb 2019 09:23:10: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:23:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:23:10: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:23:10: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:23:10: #1 total tags in treatment: 1547400 INFO @ Fri, 15 Feb 2019 09:23:10: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:23:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:23:10: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:23:10: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:23:10: #1 total tags in treatment: 1547400 INFO @ Fri, 15 Feb 2019 09:23:10: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:23:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:23:10: #1 tags after filtering in treatment: 1547400 INFO @ Fri, 15 Feb 2019 09:23:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:23:10: #1 finished! INFO @ Fri, 15 Feb 2019 09:23:10: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:23:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:23:10: #1 tags after filtering in treatment: 1547400 INFO @ Fri, 15 Feb 2019 09:23:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:23:10: #1 finished! INFO @ Fri, 15 Feb 2019 09:23:10: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:23:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:23:10: #2 number of paired peaks: 722 WARNING @ Fri, 15 Feb 2019 09:23:10: Fewer paired peaks (722) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 722 pairs to build model! INFO @ Fri, 15 Feb 2019 09:23:10: start model_add_line... INFO @ Fri, 15 Feb 2019 09:23:10: start X-correlation... INFO @ Fri, 15 Feb 2019 09:23:10: end of X-cor INFO @ Fri, 15 Feb 2019 09:23:10: #2 finished! INFO @ Fri, 15 Feb 2019 09:23:10: #2 predicted fragment length is 174 bps INFO @ Fri, 15 Feb 2019 09:23:10: #2 alternative fragment length(s) may be 174 bps INFO @ Fri, 15 Feb 2019 09:23:10: #2.2 Generate R script for model : ERX1424546.05_model.r INFO @ Fri, 15 Feb 2019 09:23:10: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:23:10: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:23:10: #2 number of paired peaks: 722 WARNING @ Fri, 15 Feb 2019 09:23:10: Fewer paired peaks (722) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 722 pairs to build model! INFO @ Fri, 15 Feb 2019 09:23:10: start model_add_line... INFO @ Fri, 15 Feb 2019 09:23:10: start X-correlation... INFO @ Fri, 15 Feb 2019 09:23:10: #2 number of paired peaks: 722 WARNING @ Fri, 15 Feb 2019 09:23:10: Fewer paired peaks (722) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 722 pairs to build model! INFO @ Fri, 15 Feb 2019 09:23:10: start model_add_line... INFO @ Fri, 15 Feb 2019 09:23:10: end of X-cor INFO @ Fri, 15 Feb 2019 09:23:10: #2 finished! INFO @ Fri, 15 Feb 2019 09:23:10: #2 predicted fragment length is 174 bps INFO @ Fri, 15 Feb 2019 09:23:10: #2 alternative fragment length(s) may be 174 bps INFO @ Fri, 15 Feb 2019 09:23:10: #2.2 Generate R script for model : ERX1424546.10_model.r INFO @ Fri, 15 Feb 2019 09:23:10: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:23:10: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:23:11: start X-correlation... INFO @ Fri, 15 Feb 2019 09:23:11: end of X-cor INFO @ Fri, 15 Feb 2019 09:23:11: #2 finished! INFO @ Fri, 15 Feb 2019 09:23:11: #2 predicted fragment length is 174 bps INFO @ Fri, 15 Feb 2019 09:23:11: #2 alternative fragment length(s) may be 174 bps INFO @ Fri, 15 Feb 2019 09:23:11: #2.2 Generate R script for model : ERX1424546.20_model.r INFO @ Fri, 15 Feb 2019 09:23:11: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:23:11: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:23:18: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:23:18: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:23:18: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write output xls file... ERX1424546.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write output xls file... ERX1424546.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write peak in narrowPeak format file... ERX1424546.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write peak in narrowPeak format file... ERX1424546.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write summits bed file... ERX1424546.05_summits.bed INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write summits bed file... ERX1424546.10_summits.bed INFO @ Fri, 15 Feb 2019 09:23:20: Done! INFO @ Fri, 15 Feb 2019 09:23:20: Done! pass1 - making usageList (16 chroms): 3 millis pass1 - making usageList (16 chroms): 4 millis pass2 - checking and writing primary data (2046 records, 4 fields): 6 millis pass2 - checking and writing primary data (2557 records, 4 fields): 6 millis INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write output xls file... ERX1424546.20_peaks.xls CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write peak in narrowPeak format file... ERX1424546.20_peaks.narrowPeak CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:23:20: #4 Write summits bed file... ERX1424546.20_summits.bed INFO @ Fri, 15 Feb 2019 09:23:20: Done! pass1 - making usageList (16 chroms): 3 millis pass2 - checking and writing primary data (1490 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。