Job ID = 11633932 sra ファイルのダウンロード中... Completed: 151276K bytes transferred in 4 seconds (252361K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 4533377 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1424519/ERR1353044.sra Written 4533377 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1424519/ERR1353044.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:38 4533377 reads; of these: 4533377 (100.00%) were unpaired; of these: 2804844 (61.87%) aligned 0 times 1191190 (26.28%) aligned exactly 1 time 537343 (11.85%) aligned >1 times 38.13% overall alignment rate Time searching: 00:00:38 Overall time: 00:00:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 627949 / 1728533 = 0.3633 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:21:26: # Command line: callpeak -t ERX1424519.bam -f BAM -g 12100000 -n ERX1424519.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1424519.05 # format = BAM # ChIP-seq file = ['ERX1424519.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:21:26: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:21:26: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:21:26: # Command line: callpeak -t ERX1424519.bam -f BAM -g 12100000 -n ERX1424519.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1424519.20 # format = BAM # ChIP-seq file = ['ERX1424519.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:21:26: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:21:26: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:21:26: # Command line: callpeak -t ERX1424519.bam -f BAM -g 12100000 -n ERX1424519.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1424519.10 # format = BAM # ChIP-seq file = ['ERX1424519.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:21:26: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:21:26: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:21:33: 1000000 INFO @ Fri, 15 Feb 2019 09:21:33: 1000000 INFO @ Fri, 15 Feb 2019 09:21:33: 1000000 INFO @ Fri, 15 Feb 2019 09:21:33: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 09:21:33: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 09:21:33: #1 total tags in treatment: 1100584 INFO @ Fri, 15 Feb 2019 09:21:33: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:33: #1 tags after filtering in treatment: 1100584 INFO @ Fri, 15 Feb 2019 09:21:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:33: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:33: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:33: #2 number of paired peaks: 399 WARNING @ Fri, 15 Feb 2019 09:21:33: Fewer paired peaks (399) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 399 pairs to build model! INFO @ Fri, 15 Feb 2019 09:21:33: start model_add_line... INFO @ Fri, 15 Feb 2019 09:21:33: start X-correlation... INFO @ Fri, 15 Feb 2019 09:21:33: end of X-cor INFO @ Fri, 15 Feb 2019 09:21:33: #2 finished! INFO @ Fri, 15 Feb 2019 09:21:33: #2 predicted fragment length is 154 bps INFO @ Fri, 15 Feb 2019 09:21:33: #2 alternative fragment length(s) may be 154 bps INFO @ Fri, 15 Feb 2019 09:21:33: #2.2 Generate R script for model : ERX1424519.05_model.r INFO @ Fri, 15 Feb 2019 09:21:33: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:21:33: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:21:34: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 09:21:34: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 09:21:34: #1 total tags in treatment: 1100584 INFO @ Fri, 15 Feb 2019 09:21:34: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:34: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 09:21:34: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 09:21:34: #1 total tags in treatment: 1100584 INFO @ Fri, 15 Feb 2019 09:21:34: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:34: #1 tags after filtering in treatment: 1100584 INFO @ Fri, 15 Feb 2019 09:21:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:34: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:34: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:34: #1 tags after filtering in treatment: 1100584 INFO @ Fri, 15 Feb 2019 09:21:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:34: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:34: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:34: #2 number of paired peaks: 399 WARNING @ Fri, 15 Feb 2019 09:21:34: Fewer paired peaks (399) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 399 pairs to build model! INFO @ Fri, 15 Feb 2019 09:21:34: start model_add_line... INFO @ Fri, 15 Feb 2019 09:21:34: #2 number of paired peaks: 399 WARNING @ Fri, 15 Feb 2019 09:21:34: Fewer paired peaks (399) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 399 pairs to build model! INFO @ Fri, 15 Feb 2019 09:21:34: start model_add_line... INFO @ Fri, 15 Feb 2019 09:21:34: start X-correlation... INFO @ Fri, 15 Feb 2019 09:21:34: start X-correlation... INFO @ Fri, 15 Feb 2019 09:21:34: end of X-cor INFO @ Fri, 15 Feb 2019 09:21:34: #2 finished! INFO @ Fri, 15 Feb 2019 09:21:34: #2 predicted fragment length is 154 bps INFO @ Fri, 15 Feb 2019 09:21:34: #2 alternative fragment length(s) may be 154 bps INFO @ Fri, 15 Feb 2019 09:21:34: #2.2 Generate R script for model : ERX1424519.10_model.r INFO @ Fri, 15 Feb 2019 09:21:34: end of X-cor INFO @ Fri, 15 Feb 2019 09:21:34: #2 finished! INFO @ Fri, 15 Feb 2019 09:21:34: #2 predicted fragment length is 154 bps INFO @ Fri, 15 Feb 2019 09:21:34: #2 alternative fragment length(s) may be 154 bps INFO @ Fri, 15 Feb 2019 09:21:34: #2.2 Generate R script for model : ERX1424519.20_model.r INFO @ Fri, 15 Feb 2019 09:21:34: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:21:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:21:34: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:21:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:21:37: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:21:38: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:21:38: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write output xls file... ERX1424519.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write peak in narrowPeak format file... ERX1424519.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write summits bed file... ERX1424519.05_summits.bed INFO @ Fri, 15 Feb 2019 09:21:39: Done! pass1 - making usageList (17 chroms): 3 millis pass2 - checking and writing primary data (1002 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write output xls file... ERX1424519.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write peak in narrowPeak format file... ERX1424519.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write summits bed file... ERX1424519.20_summits.bed INFO @ Fri, 15 Feb 2019 09:21:39: Done! pass1 - making usageList (16 chroms): 3 millis pass2 - checking and writing primary data (534 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write output xls file... ERX1424519.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write peak in narrowPeak format file... ERX1424519.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:21:39: #4 Write summits bed file... ERX1424519.10_summits.bed INFO @ Fri, 15 Feb 2019 09:21:39: Done! pass1 - making usageList (16 chroms): 3 millis pass2 - checking and writing primary data (719 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。