Job ID = 11633931 sra ファイルのダウンロード中... Completed: 103642K bytes transferred in 4 seconds (183188K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 2955570 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1424518/ERR1353043.sra Written 2955570 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1424518/ERR1353043.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:25 2955570 reads; of these: 2955570 (100.00%) were unpaired; of these: 1865873 (63.13%) aligned 0 times 761044 (25.75%) aligned exactly 1 time 328653 (11.12%) aligned >1 times 36.87% overall alignment rate Time searching: 00:00:25 Overall time: 00:00:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 316934 / 1089697 = 0.2908 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:20:59: # Command line: callpeak -t ERX1424518.bam -f BAM -g 12100000 -n ERX1424518.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1424518.10 # format = BAM # ChIP-seq file = ['ERX1424518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:20:59: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:20:59: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:20:59: # Command line: callpeak -t ERX1424518.bam -f BAM -g 12100000 -n ERX1424518.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1424518.20 # format = BAM # ChIP-seq file = ['ERX1424518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:20:59: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:20:59: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:20:59: # Command line: callpeak -t ERX1424518.bam -f BAM -g 12100000 -n ERX1424518.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1424518.05 # format = BAM # ChIP-seq file = ['ERX1424518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:20:59: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:20:59: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:21:05: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 09:21:05: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 09:21:05: #1 total tags in treatment: 772763 INFO @ Fri, 15 Feb 2019 09:21:05: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:05: #1 tags after filtering in treatment: 772763 INFO @ Fri, 15 Feb 2019 09:21:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:05: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:05: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:05: #2 number of paired peaks: 393 WARNING @ Fri, 15 Feb 2019 09:21:05: Fewer paired peaks (393) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 393 pairs to build model! INFO @ Fri, 15 Feb 2019 09:21:05: start model_add_line... INFO @ Fri, 15 Feb 2019 09:21:05: start X-correlation... INFO @ Fri, 15 Feb 2019 09:21:05: end of X-cor INFO @ Fri, 15 Feb 2019 09:21:05: #2 finished! INFO @ Fri, 15 Feb 2019 09:21:05: #2 predicted fragment length is 156 bps INFO @ Fri, 15 Feb 2019 09:21:05: #2 alternative fragment length(s) may be 156 bps INFO @ Fri, 15 Feb 2019 09:21:05: #2.2 Generate R script for model : ERX1424518.05_model.r INFO @ Fri, 15 Feb 2019 09:21:05: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:21:05: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:21:05: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 09:21:05: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 09:21:05: #1 total tags in treatment: 772763 INFO @ Fri, 15 Feb 2019 09:21:05: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:05: #1 tag size is determined as 50 bps INFO @ Fri, 15 Feb 2019 09:21:05: #1 tag size = 50 INFO @ Fri, 15 Feb 2019 09:21:05: #1 total tags in treatment: 772763 INFO @ Fri, 15 Feb 2019 09:21:05: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:21:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:21:06: #1 tags after filtering in treatment: 772763 INFO @ Fri, 15 Feb 2019 09:21:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:06: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:06: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:06: #1 tags after filtering in treatment: 772763 INFO @ Fri, 15 Feb 2019 09:21:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:21:06: #1 finished! INFO @ Fri, 15 Feb 2019 09:21:06: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:21:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:21:06: #2 number of paired peaks: 393 WARNING @ Fri, 15 Feb 2019 09:21:06: Fewer paired peaks (393) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 393 pairs to build model! INFO @ Fri, 15 Feb 2019 09:21:06: start model_add_line... INFO @ Fri, 15 Feb 2019 09:21:06: #2 number of paired peaks: 393 WARNING @ Fri, 15 Feb 2019 09:21:06: Fewer paired peaks (393) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 393 pairs to build model! INFO @ Fri, 15 Feb 2019 09:21:06: start model_add_line... INFO @ Fri, 15 Feb 2019 09:21:06: start X-correlation... INFO @ Fri, 15 Feb 2019 09:21:06: end of X-cor INFO @ Fri, 15 Feb 2019 09:21:06: #2 finished! INFO @ Fri, 15 Feb 2019 09:21:06: #2 predicted fragment length is 156 bps INFO @ Fri, 15 Feb 2019 09:21:06: #2 alternative fragment length(s) may be 156 bps INFO @ Fri, 15 Feb 2019 09:21:06: #2.2 Generate R script for model : ERX1424518.20_model.r INFO @ Fri, 15 Feb 2019 09:21:06: start X-correlation... INFO @ Fri, 15 Feb 2019 09:21:06: end of X-cor INFO @ Fri, 15 Feb 2019 09:21:06: #2 finished! INFO @ Fri, 15 Feb 2019 09:21:06: #2 predicted fragment length is 156 bps INFO @ Fri, 15 Feb 2019 09:21:06: #2 alternative fragment length(s) may be 156 bps INFO @ Fri, 15 Feb 2019 09:21:06: #2.2 Generate R script for model : ERX1424518.10_model.r INFO @ Fri, 15 Feb 2019 09:21:06: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:21:06: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:21:06: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:21:06: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:21:08: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:21:08: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:21:08: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write output xls file... ERX1424518.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write peak in narrowPeak format file... ERX1424518.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write summits bed file... ERX1424518.05_summits.bed INFO @ Fri, 15 Feb 2019 09:21:09: Done! pass1 - making usageList (17 chroms): 3 millis pass2 - checking and writing primary data (817 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write output xls file... ERX1424518.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write peak in narrowPeak format file... ERX1424518.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write summits bed file... ERX1424518.10_summits.bed INFO @ Fri, 15 Feb 2019 09:21:09: Done! pass1 - making usageList (16 chroms): 4 millis pass2 - checking and writing primary data (610 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write output xls file... ERX1424518.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write peak in narrowPeak format file... ERX1424518.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:21:09: #4 Write summits bed file... ERX1424518.20_summits.bed INFO @ Fri, 15 Feb 2019 09:21:09: Done! pass1 - making usageList (16 chroms): 3 millis pass2 - checking and writing primary data (439 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。