Job ID = 11633921 sra ファイルのダウンロード中... Completed: 61610K bytes transferred in 4 seconds (125624K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 1998957 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420535/ERR1349011.sra Written 1998957 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420535/ERR1349011.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:20 1998957 reads; of these: 1998957 (100.00%) were unpaired; of these: 694044 (34.72%) aligned 0 times 1051302 (52.59%) aligned exactly 1 time 253611 (12.69%) aligned >1 times 65.28% overall alignment rate Time searching: 00:00:20 Overall time: 00:00:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 586082 / 1304913 = 0.4491 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:17:50: # Command line: callpeak -t ERX1420535.bam -f BAM -g 12100000 -n ERX1420535.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420535.10 # format = BAM # ChIP-seq file = ['ERX1420535.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:17:50: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:17:50: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:17:50: # Command line: callpeak -t ERX1420535.bam -f BAM -g 12100000 -n ERX1420535.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420535.20 # format = BAM # ChIP-seq file = ['ERX1420535.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:17:50: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:17:50: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:17:50: # Command line: callpeak -t ERX1420535.bam -f BAM -g 12100000 -n ERX1420535.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420535.05 # format = BAM # ChIP-seq file = ['ERX1420535.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:17:50: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:17:50: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:17:55: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:17:55: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:17:55: #1 total tags in treatment: 718831 INFO @ Fri, 15 Feb 2019 09:17:55: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:17:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:17:55: #1 tags after filtering in treatment: 718831 INFO @ Fri, 15 Feb 2019 09:17:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:17:55: #1 finished! INFO @ Fri, 15 Feb 2019 09:17:55: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:17:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:17:55: #2 number of paired peaks: 194 WARNING @ Fri, 15 Feb 2019 09:17:55: Fewer paired peaks (194) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 194 pairs to build model! INFO @ Fri, 15 Feb 2019 09:17:55: start model_add_line... INFO @ Fri, 15 Feb 2019 09:17:55: start X-correlation... INFO @ Fri, 15 Feb 2019 09:17:55: end of X-cor INFO @ Fri, 15 Feb 2019 09:17:55: #2 finished! INFO @ Fri, 15 Feb 2019 09:17:55: #2 predicted fragment length is 302 bps INFO @ Fri, 15 Feb 2019 09:17:55: #2 alternative fragment length(s) may be 3,302 bps INFO @ Fri, 15 Feb 2019 09:17:55: #2.2 Generate R script for model : ERX1420535.20_model.r INFO @ Fri, 15 Feb 2019 09:17:55: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:17:55: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:17:55: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:17:55: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:17:55: #1 total tags in treatment: 718831 INFO @ Fri, 15 Feb 2019 09:17:55: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:17:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:17:55: #1 tags after filtering in treatment: 718831 INFO @ Fri, 15 Feb 2019 09:17:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:17:55: #1 finished! INFO @ Fri, 15 Feb 2019 09:17:55: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:17:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:17:55: #2 number of paired peaks: 194 WARNING @ Fri, 15 Feb 2019 09:17:55: Fewer paired peaks (194) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 194 pairs to build model! INFO @ Fri, 15 Feb 2019 09:17:55: start model_add_line... INFO @ Fri, 15 Feb 2019 09:17:55: start X-correlation... INFO @ Fri, 15 Feb 2019 09:17:55: end of X-cor INFO @ Fri, 15 Feb 2019 09:17:55: #2 finished! INFO @ Fri, 15 Feb 2019 09:17:55: #2 predicted fragment length is 302 bps INFO @ Fri, 15 Feb 2019 09:17:55: #2 alternative fragment length(s) may be 3,302 bps INFO @ Fri, 15 Feb 2019 09:17:55: #2.2 Generate R script for model : ERX1420535.10_model.r INFO @ Fri, 15 Feb 2019 09:17:55: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:17:55: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:17:56: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:17:56: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:17:56: #1 total tags in treatment: 718831 INFO @ Fri, 15 Feb 2019 09:17:56: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:17:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:17:56: #1 tags after filtering in treatment: 718831 INFO @ Fri, 15 Feb 2019 09:17:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:17:56: #1 finished! INFO @ Fri, 15 Feb 2019 09:17:56: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:17:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:17:56: #2 number of paired peaks: 194 WARNING @ Fri, 15 Feb 2019 09:17:56: Fewer paired peaks (194) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 194 pairs to build model! INFO @ Fri, 15 Feb 2019 09:17:56: start model_add_line... INFO @ Fri, 15 Feb 2019 09:17:56: start X-correlation... INFO @ Fri, 15 Feb 2019 09:17:56: end of X-cor INFO @ Fri, 15 Feb 2019 09:17:56: #2 finished! INFO @ Fri, 15 Feb 2019 09:17:56: #2 predicted fragment length is 302 bps INFO @ Fri, 15 Feb 2019 09:17:56: #2 alternative fragment length(s) may be 3,302 bps INFO @ Fri, 15 Feb 2019 09:17:56: #2.2 Generate R script for model : ERX1420535.05_model.r INFO @ Fri, 15 Feb 2019 09:17:56: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:17:56: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:17:59: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:17:59: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:17:59: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write output xls file... ERX1420535.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write peak in narrowPeak format file... ERX1420535.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write summits bed file... ERX1420535.10_summits.bed INFO @ Fri, 15 Feb 2019 09:18:00: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (114 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write output xls file... ERX1420535.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write peak in narrowPeak format file... ERX1420535.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write summits bed file... ERX1420535.20_summits.bed INFO @ Fri, 15 Feb 2019 09:18:00: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (34 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write output xls file... ERX1420535.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write peak in narrowPeak format file... ERX1420535.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:18:00: #4 Write summits bed file... ERX1420535.05_summits.bed INFO @ Fri, 15 Feb 2019 09:18:00: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (265 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。