Job ID = 11633905 sra ファイルのダウンロード中... Completed: 3992K bytes transferred in 2 seconds (14063K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 120971 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420519/ERR1348995.sra Written 120971 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420519/ERR1348995.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:02 120971 reads; of these: 120971 (100.00%) were unpaired; of these: 36127 (29.86%) aligned 0 times 60472 (49.99%) aligned exactly 1 time 24372 (20.15%) aligned >1 times 70.14% overall alignment rate Time searching: 00:00:02 Overall time: 00:00:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 7067 / 84844 = 0.0833 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:19:22: # Command line: callpeak -t ERX1420519.bam -f BAM -g 12100000 -n ERX1420519.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420519.20 # format = BAM # ChIP-seq file = ['ERX1420519.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:19:22: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:19:22: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:19:22: # Command line: callpeak -t ERX1420519.bam -f BAM -g 12100000 -n ERX1420519.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420519.10 # format = BAM # ChIP-seq file = ['ERX1420519.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:19:22: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:19:22: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:19:22: # Command line: callpeak -t ERX1420519.bam -f BAM -g 12100000 -n ERX1420519.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420519.05 # format = BAM # ChIP-seq file = ['ERX1420519.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:19:22: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:19:22: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:19:23: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:19:23: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:19:23: #1 total tags in treatment: 77777 INFO @ Fri, 15 Feb 2019 09:19:23: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:19:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:19:23: #1 tags after filtering in treatment: 77777 INFO @ Fri, 15 Feb 2019 09:19:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:19:23: #1 finished! INFO @ Fri, 15 Feb 2019 09:19:23: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:19:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:19:23: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:19:23: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:19:23: #1 total tags in treatment: 77777 INFO @ Fri, 15 Feb 2019 09:19:23: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:19:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:19:23: #2 number of paired peaks: 188 WARNING @ Fri, 15 Feb 2019 09:19:23: Fewer paired peaks (188) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 188 pairs to build model! INFO @ Fri, 15 Feb 2019 09:19:23: start model_add_line... INFO @ Fri, 15 Feb 2019 09:19:23: start X-correlation... INFO @ Fri, 15 Feb 2019 09:19:23: #1 tags after filtering in treatment: 77777 INFO @ Fri, 15 Feb 2019 09:19:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:19:23: #1 finished! INFO @ Fri, 15 Feb 2019 09:19:23: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:19:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:19:23: end of X-cor INFO @ Fri, 15 Feb 2019 09:19:23: #2 finished! INFO @ Fri, 15 Feb 2019 09:19:23: #2 predicted fragment length is 212 bps INFO @ Fri, 15 Feb 2019 09:19:23: #2 alternative fragment length(s) may be 154,191,212,288 bps INFO @ Fri, 15 Feb 2019 09:19:23: #2.2 Generate R script for model : ERX1420519.20_model.r INFO @ Fri, 15 Feb 2019 09:19:23: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:19:23: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:19:23: #1 total tags in treatment: 77777 INFO @ Fri, 15 Feb 2019 09:19:23: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:19:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:19:23: #1 tags after filtering in treatment: 77777 INFO @ Fri, 15 Feb 2019 09:19:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:19:23: #1 finished! INFO @ Fri, 15 Feb 2019 09:19:23: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:19:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:19:23: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:19:23: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:19:23: #2 number of paired peaks: 188 WARNING @ Fri, 15 Feb 2019 09:19:23: Fewer paired peaks (188) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 188 pairs to build model! INFO @ Fri, 15 Feb 2019 09:19:23: start model_add_line... INFO @ Fri, 15 Feb 2019 09:19:23: start X-correlation... INFO @ Fri, 15 Feb 2019 09:19:23: end of X-cor INFO @ Fri, 15 Feb 2019 09:19:23: #2 finished! INFO @ Fri, 15 Feb 2019 09:19:23: #2 predicted fragment length is 212 bps INFO @ Fri, 15 Feb 2019 09:19:23: #2 alternative fragment length(s) may be 154,191,212,288 bps INFO @ Fri, 15 Feb 2019 09:19:23: #2.2 Generate R script for model : ERX1420519.10_model.r INFO @ Fri, 15 Feb 2019 09:19:23: #2 number of paired peaks: 188 WARNING @ Fri, 15 Feb 2019 09:19:23: Fewer paired peaks (188) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 188 pairs to build model! INFO @ Fri, 15 Feb 2019 09:19:23: start model_add_line... INFO @ Fri, 15 Feb 2019 09:19:23: start X-correlation... INFO @ Fri, 15 Feb 2019 09:19:23: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:19:23: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:19:23: end of X-cor INFO @ Fri, 15 Feb 2019 09:19:23: #2 finished! INFO @ Fri, 15 Feb 2019 09:19:23: #2 predicted fragment length is 212 bps INFO @ Fri, 15 Feb 2019 09:19:23: #2 alternative fragment length(s) may be 154,191,212,288 bps INFO @ Fri, 15 Feb 2019 09:19:23: #2.2 Generate R script for model : ERX1420519.05_model.r INFO @ Fri, 15 Feb 2019 09:19:23: #3 Call peaks... INFO @ Fri, 15 Feb 2019 09:19:23: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 15 Feb 2019 09:19:23: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:19:23: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:19:23: #3 Call peaks for each chromosome... INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write output xls file... ERX1420519.20_peaks.xls INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write peak in narrowPeak format file... ERX1420519.20_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write summits bed file... ERX1420519.20_summits.bed INFO @ Fri, 15 Feb 2019 09:19:23: Done! INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write output xls file... ERX1420519.10_peaks.xls INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write peak in narrowPeak format file... ERX1420519.10_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write output xls file... ERX1420519.05_peaks.xls INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write summits bed file... ERX1420519.10_summits.bed INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write peak in narrowPeak format file... ERX1420519.05_peaks.narrowPeak INFO @ Fri, 15 Feb 2019 09:19:23: Done! INFO @ Fri, 15 Feb 2019 09:19:23: #4 Write summits bed file... ERX1420519.05_summits.bed INFO @ Fri, 15 Feb 2019 09:19:23: Done! pass1 - making usageList (1 chroms): 8 millis pass2 - checking and writing primary data (3 records, 4 fields): 5 millis pass1 - making usageList (1 chroms): 7 millis pass2 - checking and writing primary data (7 records, 4 fields): 7 millis CompletedMACS2peakCalling CompletedMACS2peakCalling pass1 - making usageList (13 chroms): 3 millis pass2 - checking and writing primary data (50 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。