Job ID = 11633869 sra ファイルのダウンロード中... Completed: 6885K bytes transferred in 2 seconds (20641K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 207649 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420486/ERR1348962.sra Written 207649 spots for /home/okishinya/chipatlas/results/sacCer3/ERX1420486/ERR1348962.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:02 207649 reads; of these: 207649 (100.00%) were unpaired; of these: 45640 (21.98%) aligned 0 times 132626 (63.87%) aligned exactly 1 time 29383 (14.15%) aligned >1 times 78.02% overall alignment rate Time searching: 00:00:02 Overall time: 00:00:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 6475 / 162009 = 0.0400 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 15 Feb 2019 09:15:42: # Command line: callpeak -t ERX1420486.bam -f BAM -g 12100000 -n ERX1420486.10 -q 1e-10 # ARGUMENTS LIST: # name = ERX1420486.10 # format = BAM # ChIP-seq file = ['ERX1420486.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:15:42: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:15:42: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:15:42: # Command line: callpeak -t ERX1420486.bam -f BAM -g 12100000 -n ERX1420486.05 -q 1e-05 # ARGUMENTS LIST: # name = ERX1420486.05 # format = BAM # ChIP-seq file = ['ERX1420486.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:15:42: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:15:42: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:15:42: # Command line: callpeak -t ERX1420486.bam -f BAM -g 12100000 -n ERX1420486.20 -q 1e-20 # ARGUMENTS LIST: # name = ERX1420486.20 # format = BAM # ChIP-seq file = ['ERX1420486.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 15 Feb 2019 09:15:42: #1 read tag files... INFO @ Fri, 15 Feb 2019 09:15:42: #1 read treatment tags... INFO @ Fri, 15 Feb 2019 09:15:43: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:15:43: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:15:43: #1 total tags in treatment: 155534 INFO @ Fri, 15 Feb 2019 09:15:43: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:15:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:15:43: #1 tags after filtering in treatment: 155534 INFO @ Fri, 15 Feb 2019 09:15:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:15:43: #1 finished! INFO @ Fri, 15 Feb 2019 09:15:43: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:15:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:15:43: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:15:43: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:15:43: #1 total tags in treatment: 155534 INFO @ Fri, 15 Feb 2019 09:15:43: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:15:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 15 Feb 2019 09:15:43: #1 tags after filtering in treatment: 155534 INFO @ Fri, 15 Feb 2019 09:15:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:15:43: #1 finished! INFO @ Fri, 15 Feb 2019 09:15:43: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:15:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 15 Feb 2019 09:15:43: #2 number of paired peaks: 5 WARNING @ Fri, 15 Feb 2019 09:15:43: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:15:43: Process for pairing-model is terminated! INFO @ Fri, 15 Feb 2019 09:15:43: #1 tag size is determined as 51 bps INFO @ Fri, 15 Feb 2019 09:15:43: #1 tag size = 51 INFO @ Fri, 15 Feb 2019 09:15:43: #1 total tags in treatment: 155534 INFO @ Fri, 15 Feb 2019 09:15:43: #1 user defined the maximum tags... INFO @ Fri, 15 Feb 2019 09:15:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cat: ERX1420486.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Fri, 15 Feb 2019 09:15:43: #2 number of paired peaks: 5 WARNING @ Fri, 15 Feb 2019 09:15:43: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:15:43: Process for pairing-model is terminated! INFO @ Fri, 15 Feb 2019 09:15:43: #1 tags after filtering in treatment: 155534 INFO @ Fri, 15 Feb 2019 09:15:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 15 Feb 2019 09:15:43: #1 finished! INFO @ Fri, 15 Feb 2019 09:15:43: #2 Build Peak Model... INFO @ Fri, 15 Feb 2019 09:15:43: #2 looking for paired plus/minus strand peaks... cat: ERX1420486.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Fri, 15 Feb 2019 09:15:43: #2 number of paired peaks: 5 WARNING @ Fri, 15 Feb 2019 09:15:43: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 15 Feb 2019 09:15:43: Process for pairing-model is terminated! cat: ERX1420486.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 15 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 16 millis rm: cannot remove `ERX1420486.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420486.10_*.xls': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `ERX1420486.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 13 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove `ERX1420486.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420486.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420486.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `ERX1420486.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420486.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `ERX1420486.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。